Difference between revisions of "Team:IIT Kanpur/Human Practices"

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<h3>Our Endeavours</h3>
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<h3>The Experiences</h3>
  
<button class="collapsible">Session at Prayas</button>
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<button class="collapsible">Visiting Unique Agencies Sewage Treatment Plant</button>
 
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   <p style="font-size:120%;">As part of our initiative to spread awareness about synthetic biology, we organized a lecture on ‘Introduction to Bioengineering’ for the young students at Prayas, IIT Kanpur. <br>Prayas IIT Kanpur is an endeavor undertaken by IIT Kanpur students, mainly focused on providing education to the marginalized in and around the campus, to increase awareness so that they can make more informed and rational choices about their career. Presently a club under the Presidential Council in the Students' Gymkhana, IIT Kanpur, it’s a voluntary service to promote the notion of 'Education for All' as a sustainable means of development.<br>
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   <p style="font-size:120%;">Naturally human waste takes around 4-5 days to completely decompose. Nowadays sewage is treated in plants where it takes about 4-5 hours to degrade. To understand better how water from households is cleaned, we organized an industry visit to Unique water industries in Kanpur. <br>
In India, Bioengineering is not seen as a lucrative and fulfilling career option despite how useful and creatively involving the field is. It is partially due to lack of exposure to the various possibilities in the area and due to lack of awareness of its accomplishments. We, as a team involved in synthetic biology, believe that it is our responsibility to promote awareness. Prayas is our first step towards accomplishing this aim. <br><br>
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After an in depth conversation with the company official, it became clear that biological processes are at the heart of almost every sewage treatment process. The industry which we visited uses a technique called RBC (Rotating biological contactor)<br>
In the lecture that we delivered, we motivated kids about the uses of biology by taking them on tour through the cells and its various molecular machines. Then we made analogies between known phenomenon like exothermic combustion of petrol and how engineering principles make it useful (i.e., run a car) and then connected how the intricate machines of biology and engineering principles could connect to help ease our lives. <br>The kids got excited and were able to follow through the entire lecture. We showed them many practical applications of Bioengineering not just related to molecular biology but also about fields like Tissue engineering and Soft Robotics. The whole experience was fulfilling for us as well as for the kids. In the doubts and question-answer session the kids exhibited great enthusiasm and asked questions on how they could do such things, and interestingly, they even suggested their mechanisms on how they would solve some problems (like an artificial heart).
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This plant uses a 3-Rotor model for RBC. The first rotor introduces the bacteria into the sewage and aerates the sewage. Thereafter, the sewage is fed to a second chamber where the second rotor carries out the main aerobic reaction, where bacteria degrades . Maximum time is spent in the second chamber where the majority of the cleaning takes place. Eventually the third rotor removes dead bacteria from the water, after which the treated water is collected based on laminar flow. This water is fit for use in basic horticulture.<br>
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Other methods include sequential batch reactor, MBBR(Moving bed biofilm reactor) etc. There are issues regarding the complex procedure and huge amount of energy required to mix semi solid sludge. RBC is the simple to install and has low maintenance costs. <br>
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From our experiments we found out that the presence of (%) SDS reduces growth of E.Coli. Treating water for SDS before feeding to the RBC may improve the efficiency of the water that the RBC currently provides. <br>
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One more chamber can be connected where the first rotor is coated with the SDS-a protein obtained by the extracellular expression which will degrade the SDS in the primary discharge. Such subtle changes can bring out a drastic improvement in the water purification techniques, especially when dealing with SDS-Rich discharges from Hotel and Hospital discharges. The SDS concentration in such discharges reaches 8-9% which is difficult to neutralize even using chemical processes. Such an improvement can lead to a more efficient way of dealing high SDS content discharges using the RBC technique.<br>
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<button class="collapsible">Lecture Series</button>
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<button class="collapsible">Meeting with Dr. Purnendu Bose</button>
 
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   <p style="font-size:120%;">To spread awareness about iGEM and our own project, our team conducted a lecture series for the campus community. <br>The series introduced the students to iGEM and the nature of work done in the same. We explained the requirements and practices one should follow to pitch an idea successfully and work on it in a presentable manner. <br>In the subsequent lectures we exemplified the process using our project. This helped us in harboring the students’ interest and exposed them to the nature of work being done. We found it to be an effective way to help students form future iGEM teams and motivate them to use their intellect to create innovative solutions.
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   <p style="font-size:120%;">As an academic venture, our team felt that discussing our ideas with fellow academicians would contribute to the growth of our project and also help us from pursue new lines of thought that we had not yet explored. To address this we felt that experts with knowledge on both environmental engineering and experience in practical implementations would be of great value.<br>
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We met with Dr. Purnendu Bose who is a Professor at the Dept. of Civil Engineering at IIT Kanpur and he specializes in environmental engineering. Our discussion was a fruitful exchange where we introduced our project about how we plan to clean detergent-laden water. We were unclear of any useful implementations.<br>
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He told us about the current problem faced by companies making Washing machines. People who are the target customers for these companies have the money to afford washing machines, but they still do not get personal washing machines due to lack of availability of clean water. He then suggested that we can model our product along the lines of this problem. It proved to be a crucial piece of information, and the whole conversation proceeded to ideate a washing machine add-on that recycles the water that it uses, and hence the water requirement for the washing machine decreases a lot. This model could be used generally to cut water wastage at a significant rate.
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<button class="collapsible">Meeting with Dr. Rajiv Sinha</button>
 
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   <p style="font-size:120%;">iGEM requires teams to figure out a problem themselves which they feel requires immediate attention and then ideate a novel synthetic biological system to tackle it.<br>
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   <p style="font-size:120%;">We met Dr. Rajiv Sinha who is also a Professor at the Dept. of Civil Engg. His expertise lies in Bioremediation, and his advice and suggestions were very helpful to us. Our problem was that though we had some idea of how our bacteria would work in the controlled settings of our lab, we did not know what challenges we would face when applying the same to water samples from the real world. One particular problem we had was that our enzyme might not work in the pH conditions, the presence of other ions etc. and so to understand real-world challenges we shared this problem with him.<br>
To give the students a hands-on experience of the ideation phase of iGEM we conducted an ideation hackathon where participants were required to submit an idea to curb the problem of water scarcity. <br>
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He suggested that we test our protein’s function on SDS in the presence of major ions in water and see the concentration's effect on protein function. He guided us to check protein functionality with each of the major ions separately, to better assess the effects of each ion individually.  We could then see the level of these prominent ions in water bodies in different places and formulate our strategy for implementing our device in the future.
They were required to submit a possible working model that applies their idea in real life setting along with the principles involved and the corresponding literature. This opportunity allowed the students to get acquainted with the practices like literature survey and innovation on practical grounds which are crucial during the ideation phase of any innovative endeavor.
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Another important point he reiterated was, that instead of targeting the affected water bodies, it would be more efficient if we could tackle the problem right at the source itself. He explained to us how that model would not only bring down cost, but help avoid the unpredictability of the composition of water being cleansed.<br>
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<button class="collapsible">Other Activities</button>
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<button class="collapsible">AutoCAD Model - Our Design</button>
 
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   <p style="font-size:120%;">The team also met experts and people working in the field to gain more experience and look at the problem from different angles and thus, design a better working model to tackle the problem of Sodium Dodecyl Sulfate contamination in water bodies. The members met Dr. Purnendu Bose and Dr. Rajiv Sinha who are Professors at Dept. of Civil Engineering, IIT Kanpur and specialize in Environmental Sciences and Bioremediation respectively. They also met experts from Unique Agencies to know the intricacies of a real-world water bioremediation plant.
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   <p style="font-size:120%;">To be soon uploaded...
 
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Read more at our <a href="https://2018.igem.org/Team:IIT_Kanpur/Human_Practices">Integrated Human Practices</a>.
 
 
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Revision as of 10:58, 17 October 2018

If it stands the test of public scrutiny, do it... if it doesn't stand the test of public scrutiny then don't do it.

-Ratan Tata
To figure out what those affected by the cause you’re working for require, and then being able to satisfy that, is the true mark of an innovator. Thus, getting out of labs and spending time with those who already have the experience is a boost every team undertakes.

The Experiences

Naturally human waste takes around 4-5 days to completely decompose. Nowadays sewage is treated in plants where it takes about 4-5 hours to degrade. To understand better how water from households is cleaned, we organized an industry visit to Unique water industries in Kanpur.
After an in depth conversation with the company official, it became clear that biological processes are at the heart of almost every sewage treatment process. The industry which we visited uses a technique called RBC (Rotating biological contactor)
This plant uses a 3-Rotor model for RBC. The first rotor introduces the bacteria into the sewage and aerates the sewage. Thereafter, the sewage is fed to a second chamber where the second rotor carries out the main aerobic reaction, where bacteria degrades . Maximum time is spent in the second chamber where the majority of the cleaning takes place. Eventually the third rotor removes dead bacteria from the water, after which the treated water is collected based on laminar flow. This water is fit for use in basic horticulture.
Other methods include sequential batch reactor, MBBR(Moving bed biofilm reactor) etc. There are issues regarding the complex procedure and huge amount of energy required to mix semi solid sludge. RBC is the simple to install and has low maintenance costs.
From our experiments we found out that the presence of (%) SDS reduces growth of E.Coli. Treating water for SDS before feeding to the RBC may improve the efficiency of the water that the RBC currently provides.
One more chamber can be connected where the first rotor is coated with the SDS-a protein obtained by the extracellular expression which will degrade the SDS in the primary discharge. Such subtle changes can bring out a drastic improvement in the water purification techniques, especially when dealing with SDS-Rich discharges from Hotel and Hospital discharges. The SDS concentration in such discharges reaches 8-9% which is difficult to neutralize even using chemical processes. Such an improvement can lead to a more efficient way of dealing high SDS content discharges using the RBC technique.

As an academic venture, our team felt that discussing our ideas with fellow academicians would contribute to the growth of our project and also help us from pursue new lines of thought that we had not yet explored. To address this we felt that experts with knowledge on both environmental engineering and experience in practical implementations would be of great value.
We met with Dr. Purnendu Bose who is a Professor at the Dept. of Civil Engineering at IIT Kanpur and he specializes in environmental engineering. Our discussion was a fruitful exchange where we introduced our project about how we plan to clean detergent-laden water. We were unclear of any useful implementations.
He told us about the current problem faced by companies making Washing machines. People who are the target customers for these companies have the money to afford washing machines, but they still do not get personal washing machines due to lack of availability of clean water. He then suggested that we can model our product along the lines of this problem. It proved to be a crucial piece of information, and the whole conversation proceeded to ideate a washing machine add-on that recycles the water that it uses, and hence the water requirement for the washing machine decreases a lot. This model could be used generally to cut water wastage at a significant rate.

We met Dr. Rajiv Sinha who is also a Professor at the Dept. of Civil Engg. His expertise lies in Bioremediation, and his advice and suggestions were very helpful to us. Our problem was that though we had some idea of how our bacteria would work in the controlled settings of our lab, we did not know what challenges we would face when applying the same to water samples from the real world. One particular problem we had was that our enzyme might not work in the pH conditions, the presence of other ions etc. and so to understand real-world challenges we shared this problem with him.
He suggested that we test our protein’s function on SDS in the presence of major ions in water and see the concentration's effect on protein function. He guided us to check protein functionality with each of the major ions separately, to better assess the effects of each ion individually. We could then see the level of these prominent ions in water bodies in different places and formulate our strategy for implementing our device in the future. Another important point he reiterated was, that instead of targeting the affected water bodies, it would be more efficient if we could tackle the problem right at the source itself. He explained to us how that model would not only bring down cost, but help avoid the unpredictability of the composition of water being cleansed.

To be soon uploaded...