Difference between revisions of "Team:KUAS Korea/Design"

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        <td class="align-middle text-center section-header"><h3>Design</h3></td>
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<h1>Design</h1>
 
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Design is the first step in the design-build-test cycle in engineering and synthetic biology. Use this page to describe the process that you used in the design of your parts. You should clearly explain the engineering principles used to design your project.
 
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This page is different to the "Applied Design Award" page. Please see the <a href="https://2018.igem.org/Team:KUAS_Korea/Applied_Design">Applied Design</a> page for more information on how to compete for that award.
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                <br><br><br>
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                <center><h4><font size = 6.5><strong>B</strong>acterial <strong>E</strong>volutionary <strong>G</strong>ame <strong>S</strong>imulation (<strong>BEGS</strong>)</font><br><font size = 3><i>for Snowdrift, Harmony, Stag Hunt and Prisoner's Dilemma Games</i></font></h4>
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                <br><br><br>
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                <p>이미지가 들어갈 자리입니다 호호</p><br><br><br>
  
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                <li>Producer strain (“cooperator”): display of β-glucosidase on the cell surface</li>
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                <li>Cheater strain: expression of GFP<sub>uv</sub> in the cell, which acts as a reporter gene</li><br><br>
  
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                <ol>The mechanism is as follows.<br>
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                <li>The producer (cooperator) constitutively expresses beta-glucosidase on its surface and degrades the cellobiose. </li>
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                <li>The cooperator and the cheater take them as energy source and the cheater expresses GFPuv as a reporter gene when it takes up the glucose. </li>
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                <li>As the ratio between the cheater and cooperator changes, the total number of cooperator and cheater changes at the end.</li></ol>
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                </div>
  
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            </header>
<h3>What should this page contain?</h3>
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        </div>
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    </section>
<li>Explanation of the engineering principles your team used in your design</li>
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</main>
<li>Discussion of the design iterations your team went through</li>
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<li>Experimental plan to test your designs</li>
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</ul>
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<div class="highlight decoration_A_full">
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<h3>Inspiration</h3>
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<ul>
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<li><a href="https://2016.igem.org/Team:MIT/Experiments/Promoters">2016 MIT</a></li>
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<li><a href="https://2016.igem.org/Team:BostonU/Proof">2016 BostonU</a></li>
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<li><a href="https://2016.igem.org/Team:NCTU_Formosa/Design">2016 NCTU Formosa</a></li>
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</ul>
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</div>
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</div>
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Revision as of 11:24, 17 October 2018

Design




Bacterial Evolutionary Game Simulation (BEGS)
for Snowdrift, Harmony, Stag Hunt and Prisoner's Dilemma Games




이미지가 들어갈 자리입니다 호호




  • Producer strain (“cooperator”): display of β-glucosidase on the cell surface
  • Cheater strain: expression of GFPuv in the cell, which acts as a reporter gene


    1. The mechanism is as follows.
    2. The producer (cooperator) constitutively expresses beta-glucosidase on its surface and degrades the cellobiose.
    3. The cooperator and the cheater take them as energy source and the cheater expresses GFPuv as a reporter gene when it takes up the glucose.
    4. As the ratio between the cheater and cooperator changes, the total number of cooperator and cheater changes at the end.

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