Difference between revisions of "Team:Jilin China"
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<p>Many strategies could be used to regulate the gene expression responding to temperature changes in bacterial cells. A well-known class of biological thermoregulatory elements includes RNA-based thermosensors (RTs), which are located in the 5'- | <p>Many strategies could be used to regulate the gene expression responding to temperature changes in bacterial cells. A well-known class of biological thermoregulatory elements includes RNA-based thermosensors (RTs), which are located in the 5'- | ||
untranslated region in mRNA. RTs could sense temperature shift and influence the mRNA stability or ribosome accessibility, thereby controlling translation efficiency.</p> | untranslated region in mRNA. RTs could sense temperature shift and influence the mRNA stability or ribosome accessibility, thereby controlling translation efficiency.</p> | ||
| − | <p>However, natural RNA-based thermosensors have complex structure and narrow sensing temperature range, which become the biggest limitation in their | + | <p>However, natural RNA-based thermosensors have complex structure and narrow sensing temperature range, which become the biggest limitation in their application. To address this issue, we have created a synthetic RNA-based thermosensor (SynRT) toolkit with different sensing temperatures. Additionally, we built a matched search engine for users. |
<p>The SynRT toolkit version has been updated from 1.0 to 3.0, and the latest version is composed of four types of SynRTs:</p> | <p>The SynRT toolkit version has been updated from 1.0 to 3.0, and the latest version is composed of four types of SynRTs:</p> | ||
</div> | </div> | ||
Revision as of 11:31, 17 October 2018
ABSTRACT
Many strategies could be used to regulate the gene expression responding to temperature changes in bacterial cells. A well-known class of biological thermoregulatory elements includes RNA-based thermosensors (RTs), which are located in the 5'- untranslated region in mRNA. RTs could sense temperature shift and influence the mRNA stability or ribosome accessibility, thereby controlling translation efficiency.
However, natural RNA-based thermosensors have complex structure and narrow sensing temperature range, which become the biggest limitation in their application. To address this issue, we have created a synthetic RNA-based thermosensor (SynRT) toolkit with different sensing temperatures. Additionally, we built a matched search engine for users.
The SynRT toolkit version has been updated from 1.0 to 3.0, and the latest version is composed of four types of SynRTs:
Hot-induced 5'UTR
Human Practices
Model
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Title