<br />  Being responsible for our earth and ourselves is our attitude towards every
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experiment. In our project, all the parts we have utilized are selected from Risk Group 1, none of
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which are with a Red Flag. What’s more, nonpathogenic bacteria, E.coli DH5 Alpha is employed as the main
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chassis for our prior design on the DNA sequence, stem-loop and enzyme. As a matter of fact, when
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using hairpins and csy4 enzyme to tune the expression of genes, it is relatively safe for
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industrialized applications and scientific research. Based on this, We designed more variants of csy4
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enzymes and hairpins. （Click <a href='https://2018.igem.org/Team:OUC-China/Design'> here </a> to see more! ）So we used these to create a toolkit named miniToe family for synthetic biology which can
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control the expression of the target gene gradient. What’s more, we used the motA gene, one of the
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genes regulating the motility of E.coli, to verify the feasibility and effectiveness of the toolkit.
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For the sake of environment, all the chassis organisms will be sterilized before being abandoned to
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prevent the genes from leaking.
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<h3>Safe lab work</h3>
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<br />  We works at a neat lab named “Lab for Microinnovation and Enterprise”, which is a
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BSL-2 laboratory. Before working in the lab, we are supposed to be trained by the skillful hands, not
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only for the general lab safety rules, but also for the standard experimental operation avoiding
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unnecessary damage. No experiment carries no risk to the experimenters, hence we are properly equipped
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with facilities, such as latex gloves, nitrile gloves, goggles, lab coats to protect us from biotic
<br />For submitting our parts to igem HQ, we first took the initiative to consult Chinese entry-exit inspection and quarantine laws and regulations about shipping DNA from China to the USA, which is forbidden without tedious red tape. Fortunately, GenScript cooperated with igem HQ forward our part sample to the USA. Ergo, we sealed dried DNA and sent it in the submission kit to GenScript, which clearly labelled as DNA without disguise. Our kit poses no risks and is perfectly safe for shipment.
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<h3>Safety form</h3>
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<br /> <a href="https://2018.igem.org/Safety/Final_Safety_Form?team_id=2615">Click here to check our safety form.</a>
Revision as of 12:14, 17 October 2018
Team OUC-China: Main
Team member
Here we are, we are OUC-iGEM. It has been 8 years since we first took part in iGEM in 2011. This time the team which is made up of 8 members pursues a more particular and creative aim. All the members met in May 2017 when we were unfamiliar with each other and knew nothing about synthetic biology. We struck to intergrate the related disciplines and during this time we became not only teammates but also good friends. We come from different colleges and have diverse specialties which ensure that we join our abilities and insights to make our goal clearer as efficiently as possible.
