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|
| Line 398: |
Line 398: |
| | <td>Notes:</td> | | <td>Notes:</td> |
| | <td> | | <td> |
| − | HERE
| + | No notes. |
| | </td> | | </td> |
| | </tr> | | </tr> |
Phage Encapsulation
2018/08/30
| Participants: |
Johann Brenner, Keno Eilers |
| Protocol: |
Alginate Encapsulation
|
Notes: |
No notes.
|
Results: |
First Test of Encapsulation, Droplets: r=30 µM, polydisperse.
|
Phage Encapsulation
30/08/2018
| Participants: |
Johann Brenner, Keno Eilers |
| Protocol: |
Alginate Encapsulation
|
Notes: |
No notes.
|
Results: |
Results: Design of own apparatus and first 3D testprints, filament evaluation (PLA, PMMA).
|
Phage Encapsulation
2018/09/02
| Participants: |
Johann Brenner, Keno Eilers |
| Protocol: |
Alginate Encapsulation
|
Notes: |
No notes.
|
Results: |
First test of 3D print, needs some updates for better fitting, PMMA seems to be best material.
|
Phage Encapsulation
2018/09/04
| Participants: |
Johann Brenner, Keno Eilers |
| Protocol: |
Alginate Encapsulation
|
Notes: |
No notes.
|
Results: |
Works better now, better assembly, Droplets look good, but a lot of aggregate, next time more CaCl2 solution.
|
Phactory purificator
2018/09/04
| Participants: |
Johann Brenner, Keno Eilers |
| Protocol: |
NAME,
|
Notes: |
No notes.
|
Results: |
Spot test positive for fractions 13-23.
|
Phage Encapsulation
2018/09/05
| Participants: |
Johann Brenner, Keno Eilers |
| Protocol: |
Alginate Encapsulation
|
Notes: |
No notes.
|
Results: |
Test of transferring Droplets in Phage Buffer --> white sediment; CaPO4 crystals.
|
Phage Encapsulation
2018/09/12
| Participants: |
Johann Brenner, Keno Eilers |
| Protocol: |
Alginate Encapsulation
|
Notes: |
No notes.
|
Results: |
Alginate solution with10 mM MgSO4, different batches with different flow rates and N2 pressure, lack of reproducibility.
|
Phage Encapsulation
2018/09/13
| Participants: |
Johann Brenner |
| Protocol: |
Alginate Encapsulation
|
Notes: |
No notes.
|
Results: |
Design and print of new apparatus which holds dispenser needle in place.
|
Phactory purificator
2018/09/13
| Participants: |
Johann Brenner, Keno Eilers |
| Protocol: |
NAME,
|
Notes: |
No notes.
|
Results: |
Spot test positive for fractions 10-23.
|
Phage Encapsulation-stain of capsules with DAPI
2018/09/14 - 2018/09/20
| Participants: |
Johann Brenner, Keno Eilers |
| Protocol: |
Alginate Encapsulation
|
Notes: |
No notes.
|
Results: |
Test of different flow rates, enhanced reproducibility.
|
Phage Encapsulation
2018/09/21 - 2018/10/01
| Participants: |
Johann Brenner, Keno Eilers |
| Protocol: |
Alginate Encapsulation
|
Notes: |
No notes.
|
Results: |
Test of different Alginate/low viscosity Alginate ratio 1.8% Alginate + 0.2%low viscosity gave the best results.
|
Phage Encapsulation-Sybr Gold stain
2018/10/02
| Participants: |
Johann Brenner, Keno Eilers |
| Protocol: |
Alginate Encapsulation
|
Notes: |
No notes.
|
Results: |
Sybr Gold stained Phages are clearly visible, next time wash away of un bound Sybr Gold.
|
Phage Encapsulation-Test of cleaning Phages
2018/10/04
| Participants: |
Johann Brenner, Keno Eilers |
| Protocol: |
Plaque Assay,
|
Notes: |
No notes.
|
Results: |
Phages (in PBS) were centrifuged with 30 K centrifugal filters and stored in 10mM MgSO4 concentration of phages can be increased.
|
Phactory purificator
2018/10/04
| Participants: |
Johann Brenner, Keno Eilers |
| Protocol: |
NAME,
|
Notes: |
No notes.
|
Results: |
Spot test positive for several fractions, positive fractions were concentrated with centrifugal filters.
|
Phage Encapsulation with Sybr Gold staining
2018/10/06
| Participants: |
Johann Brenner, Keno Eilers |
| Protocol: |
Alginate Encapsulation
|
Notes: |
No notes.
|
Results: |
Clearly visible fluorescent signal with less background than before.
|
Test of encapsulated phages in HCl(aq)
2018/10/06
| Participants: |
Johann Brenner, Keno Eilers |
| Protocol: |
NAME,
|
Notes: |
No notes.
|
Results: |
Phages were monitored over 7h in HCl ph1, microspheres stay the same size over the time.
|
Test of Phages droplets in SGF and SIF: Phage Encapsulation
2018/10/07 - 2018/10/14
| Participants: |
Johann Brenner, Keno Eilers |
| Protocol: |
NAME, Encapsulated phages in SGF and SIF
|
Notes: |
No notes.
|
Results: |
Capsules show enhanced stability in SGF and high release of active phages in SIF.
|
