Difference between revisions of "Team:ZJU-China/Collaborations"

Teams tending to solve practically social problems may meet problems when perform their integrated Human Practice. This year, in line with the principle of “to be more responsible and reflect more”, we take part in the research conducted by Tianjin University on the transformation of biotechnology achievements. Through this expended research, we demonstrated the verify feasibility of the transformation of our iGEM project this year and made efforts on the practically execute solutions. Particularly in this research paper, we are responsible for the part of the demonstration on the transformation of our project combining the features of iGEM competition.

See more detailed information here.

SKLMT-China

In terms of collaboration, we have many communications with SKLMT-China. We used firefly luciferase report assay to characterize the strength of the promotor they provided and helped them establish the Pseudomonas fluorescence promoter pool. In detail, we electrotransform the plasmids into Pseudomonas fluorescens and used the intensity of the firefly controlled by the promoter to reflect the strength of the promoter. At the same time, SKLMT also used the reporter experiment to characterize our part. Thanks to the results came from them, we can verify our data and make the improve part section more complete.

Below is the result.

Collaborate Educate Activity: Techfest in Technology and Science Museum

Compared to the Shengxi Techfest held by ZJU-China last year, this year we conducted this activity with a hope of getting more iGEM teams involved. Here, as the collaboration we highlight above, we sincerely thanks to the participants of JNU-iGEM, ZJUT-iGEM, HFLS_ZhejiangUnited, Worldshaper-XSHS, ATSWS.

With the help of these teams, we successfully held the Shengxi Techfest and won a good reputation for this annual Techfest. Hope we can do better next year!

Worldshaper-XSHS

Worldshaper-XSHS had problem in the E.coli transformation and then they came to us for help. After a brief introduction of their project, we analyzed and pointed out that it was the invalid homemade E.coli receptive state was to be blame. Hence, they did experiments in our lab by utilizing our commercial E.coli strain BL21 with a good receptive state. This attempt provided enough opportunities for them to discuss the difference between the experiments, which exerted great advantage for them to solve such problems in the nest phase. We hope they can achieve their project and take a good grade home!

Collaboration with CSU-China

Meetup helps communication much easier. This year, the student leader Xiangyu Li of CSU-China team came to our laboratory and built connections with ZJU-China. After brief presentations, we exchanged views on our respective projects. We were impressed by their ideas on cancer therapy and considered it a promising way to developed their project rely on their advantages of medicine science in CSU.

Keep in touch and inspire more!

References

[1] Michelle M. Barnhart Matthew R. Chapman. Curli Biogenesis and Function. Annual Review of Microbiology(2006):131-147

[2] CDC.Recommendations for Prevention of HIV Transmission in Health-Care Settings.MMWR,1987;36

[3] Z Botyanszki, PKR Tay, PQ Nguyen, MG Nussbaumer, NS Joshi.Engineered catalytic biofilms: Site-specific enzyme immobilization onto E. coli curli nanofibers. Biotechnology & Bioengineering, 2015, 112 (10) :2016-2024

Our Sponsors

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 			<div class="psg" >
 				<span class="psg_ttl">Project Safety</span>
-				<span class="psg_subtitle">Biofilm Scaffold</span>
+				<span class="psg_subtitle">Collaboration with Tianjin-iGEM</span>
-				<p>Our biofilm scaffold consists of curli which is the protein found on the surface of E.Coli. Curli may facilitate bacteria’s invasion into host cells and activate corresponding cytokines and inflammatory mediators in plasma. But the system we wish to produce will be ultimately cell-free and no living organisms will be included. Besides, curli will be immobilized and covered by Nafion on the electrode, so contact with curli will not happen. Therefore, biofilm scaffold is relatively safe.</p>
+				<p>Teams tending to solve practically social problems may meet problems when perform their integrated Human Practice. This year, in line with the principle of “to be more responsible and reflect more”, we take part in the research conducted by Tianjin University on the transformation of biotechnology achievements. Through this expended research, we demonstrated the verify feasibility of the transformation of our iGEM project this year and made efforts on the practically execute solutions. Particularly in this research paper, we are responsible for the part of the demonstration on the transformation of our project combining the features of iGEM competition.</p>
-				<span class="psg_subtitle">Blood Samples</span>
+<p>See more detailed information <a href="https://2018.igem.org/Team:Tianjin/Human_Practices">here</a>.</p>
-				<p>We have gotten approval from our university authority and collected some blood samples from SIR RUN RUN SHAW HOSPITAL, which is classified as a Grade 3 Class A hospital by Chinese government. The procedure of blood sampling was done by medical personnel using ideal blood collection equipment. Besides, our experiment was conducted under professional doctors' supervision and waste was collected by the specialties in the hospital. (It is also an essential part of <a href="#">Integrated Human Practice.</a>) </br></p>
+<span class="psg_subtitle">SKLMT-China</span>
-<p></br>Our project meets ethical requirements. Blood samples we got from hospital are all remaining samples after satisfying patients’ pathological diagnosis need. We keep secret of patients’ privacy information. We conduct experiments with clinical material following our country's laws and our university's rules.</br> </p>
+<p>In terms of collaboration, we have many communications with SKLMT-China. We used firefly luciferase report assay to characterize the strength of the promotor they provided and helped them establish the Pseudomonas fluorescence promoter pool. In detail, we electrotransform the plasmids into Pseudomonas fluorescens and used the intensity of the firefly controlled by the promoter to reflect the strength of the promoter. At the same time, SKLMT also used the reporter experiment to characterize our part. Thanks to the results came from them, we can verify our data and make the improve part section more complete.</p>
-<p></br>The main issue for work with blood samples is the potential for infection. Clinical samples may be contaminated with pathogens. The high risk, well known viral agents such as Human Immunodeficiency Virus (HIV), Hepatitis B virus (HBV) and Hepatitis C Virus (HCV) are not the only agents that may be present in blood. Other viruses such as HTLV1 and B19 as well as various bacterial agents may also be present. Regarding the likely incidence of a pathogen in blood samples, several factors should be considered. These include known medical history of a patient or donor, whether the samples are from individuals showing clinical symptoms of infectious disease, the incidence of the various pathogens that are endemic in the local population or donor group and the type of sample. Fortunately, SIR RUN RUN SHAW HOSPITAL offered us the physiological indices of blood samples and other relevant information. So, the samples we used are from formal approach and relatively less dangerous. To minimize the risks，we have made Standard Operating Procedure (SOP) which is strictly followed in our iGEM lab.</p>
+<p>Below is the result.</p>
-				<span class="psg_subtitle">Standard Operating Procedure (SOP)</span>
+<img style="width:80% !important" src="https://static.igem.org/mediawiki/2018/b/b1/T--ZJU-China--SKMLT.png" />
-				<p>As insurance, Blood samples from all patients should be considered infective. The following precautions followed in our lab are also recommended for all health-care workers in clinical laboratories.</p>
-				<p style="padding-left:.5em; margin-top:.5em;">1. All the experiments where blood is involved must be conducted in a separate room within the laboratory.</p>
+<span class="psg_subtitle">Collaborate Educate Activity: Techfest in Technology and Science Museum</span>
-<p style="padding-left:.5em; margin-top:.5em;">2. All specimens of blood should be put in a well- constructed container with a secure lid to prevent leaking. Care should be taken when dealing with each specimen to avoid contaminating the outside of the container and of the laboratory form accompanying the specimen.</p>
+<p></br>Compared to the Shengxi Techfest held by ZJU-China last year, this year we conducted this activity with a hope of getting more iGEM teams involved. Here, as the collaboration we highlight above, we sincerely thanks to the participants of JNU-iGEM, ZJUT-iGEM, HFLS_ZhejiangUnited, Worldshaper-XSHS, ATSWS. </p>
-<p style="padding-left:.5em; margin-top:.5em;">3. Lab surface including correlated equipment is decontaminated with chemical disinfectant prior and after disposal.</p>
+<img style="width:80% !important" src="https://static.igem.org/mediawiki/2018/6/6d/T--ZJU-China--United.png" />
-<p style="padding-left:.5em; margin-top:.5em;">4. Every piece of material that has been in direct contact with blood should be disposed of correctly via the clinical waste route when work activities are completed.</p>
+<p></br>With the help of these teams, we successfully held the Shengxi Techfest and won a good reputation for this annual Techfest. Hope we can do better next year!</p>
-<p style="padding-left:.5em; margin-top:.5em;">5. All persons processing blood specimens should wear gloves. Gloves should be changed, and hands washed after completion of specimen processing.</p>
-<p style="padding-left:.5em; margin-top:.5em;">6. Use of sharp objects (e.g. needles, syringes, scissors) should be limited for fear of injuries and cross infection.</p>
+<span class="psg_subtitle">Worldshaper-XSHS</span>
-<p style="padding-left:.5em; margin-top:.5em;">7. All persons should wash their hands after completing blood specimens’ processing.</p>
+<p></br>Worldshaper-XSHS had problem in the E.coli transformation and then they came to us for help. After a brief introduction of their project, we analyzed and pointed out that it was the invalid homemade E.coli receptive state was to be blame. Hence, they did experiments in our lab by utilizing our commercial E.coli strain BL21 with a good receptive state. This attempt provided enough opportunities for them to discuss the difference between the experiments, which exerted great advantage for them to solve such problems in the nest phase. We hope they can achieve their project and take a good grade home!</p>
+<img style="width:80% !important" src="https://static.igem.org/mediawiki/2018/b/b4/T--ZJU-China--XSHS1.png" />
+<img style="width:80% !important" src="https://static.igem.org/mediawiki/2018/3/3b/T--ZJU-China--XSHS2.png" />
+<span class="psg_subtitle">Collaboration with CSU-China</span>
+<p></br>Meetup helps communication much easier. This year, the student leader Xiangyu Li of CSU-China team came to our laboratory and built connections with ZJU-China. After brief presentations, we exchanged views on our respective projects. We were impressed by their ideas on cancer therapy and considered it a promising way to developed their project rely on their advantages of medicine science in CSU.</p>
+<p></br>Keep in touch and inspire more!</p>
-				<span class="psg_ttl">General Safety</span>
-<p>Working with living modified organisms is always a risk. All standard precautions must be taken. Before starting to work in the lab, every member of our team was asked to attend laboratory safety lesson and take an biosafety examination.</p>
-				<span class="psg_ttl psg_subtitle">Wearing</span>
-<p>Everyone wears protective lab coats, enclosed leather shoes, gloves and safety glasses before laboratory activities.</br></p>
-<img src="https://static.igem.org/mediawiki/2018/0/01/T--ZJU-China--Safety1.jpg" style="width:500px">
-				<h5 style="color: black;">Fig.1 Working with the Clean Bench</h5>
-				<span class="psg_ttl psg_subtitle">Storage</span>
-<p>The chemical and biological reagents are stored in the proper place and suitable temperature. Hazardous or corrosive chemicals are stored separately.</br></p>
-<img src="https://static.igem.org/mediawiki/2018/2/2b/T--ZJU-China--Safety2.jpg" style="width:500px">
-				<h5 style="color: black;">Fig.2 Storage for chemical and biological reagents </h5>
-				<span class="psg_ttl psg_subtitle">Waste</span>
-<p>Waste should be classified and disposed of separately.</p>
-				<span class="psg_ttl psg_subtitle">Safety Apparatus</span>
-<p>Our iGEM lab is equipped with safety apparatus such as fire extinguishers, emergency showers, eyewash stations, and first aid kits in case of dire situations.</br> </p>
-<img src="https://static.igem.org/mediawiki/2018/d/da/T--ZJU-China--Safety3.JPG" style="width:500px">
-<h5 style="color: black;">Fig.3 The fire extinguisher</h5>
-<img src="https://static.igem.org/mediawiki/2018/5/50/T--ZJU-China--Safety4.jpg" style="width:500px">
-<h5 style="color: black;">Fig.4 The fire blanket</h5>
-<img src="https://static.igem.org/mediawiki/2018/0/07/T--ZJU-China--Safety5.JPG" style="width:500px">
-<h5 style="color: black;">Fig.5 The emergency shower</h5>
-<img src="https://static.igem.org/mediawiki/2018/0/01/T--ZJU-China--Safety6.JPG" style="width:500px">
-<h5 style="color: black;">Fig.6 The eyewash equipment</h5>
-<img src="https://static.igem.org/mediawiki/2018/0/02/T--ZJU-China--Safety7.jpg" style="width:500px">
-<h5 style="color: black;">Fig.7 The first-aid case</h5>
 				<span class="psg_ttl">References</span>
 				<p style="margin-top:.5em;">[1] Michelle M. Barnhart Matthew R. Chapman. Curli Biogenesis and Function. Annual Review of Microbiology(2006):131-147</p>