Difference between revisions of "Team:Nottingham/Model"
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<h2>Summary</h2> | <h2>Summary</h2> | ||
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| + | <div class="item">Adapted a lytic model from literature to represent our initial plan for SBRC phage (no induction model).</div> | ||
| + | <div class="item">Adapted model to include prophage induction</div> | ||
| + | <div class="item">Determined all equilibrium points of temperate phage models and relevant stability conditions.</div> | ||
| + | <div class="item">Estimated SBRC phage decay rate after noticing correlation between decay rate and burst size in a previous phage study.</div> | ||
| + | <div class="item">Determined other SBRC phage and <em>C. difficile</em> parameters from our experimental data and a literature search.</div> | ||
| + | <div class="item">Investigated and compared models analytically and numerically to determine which best suited our purpose.</div> | ||
| + | <div class="item">Proposed a model for the correlation between phage concentration and induction rate. Although this model is not influential for SBRC phage, it should be noted by other teams studying different phage.</div> | ||
| + | </div> | ||
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| + | </div> | ||
<a class="anchor" id="lytic"></a> | <a class="anchor" id="lytic"></a> | ||
Revision as of 13:21, 17 October 2018
Modelling
Aim: To model the interaction between bacteria and phage to gain insight into how we can best engineer and deliver phage to be an effective treatment against Chlostridium difficile infection.
The diagram above shows the two possible life cycles of phages. Following infection, phages following the lytic life cycle will hijack the host cell machinery to produce multiple copies of the phage proteins. These proteins are then assembled into multiple phage progeny which burst out of the host cell and go on to infect other bacterial cells. In the lysogenic life cycle, phages can integrate their genome into the host cell chromosome upon infection, where they can remain dormant for long periods of time as prophages. When conditions are favourable, usually due to host cell stress, prophage induction can occur. This is where prophages can excise from the host cell chromosome and enter the lytic life cycle leading to the production of progeny phage particles.
Click on the different sections to learn more about our modelling efforts and remember to refer back to the above diagram to help understand how our ODE models reflect the phage life cycles.
