Difference between revisions of "Team:SHSU China/Parts"
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| − | <td><a href="http://parts.igem.org/Part: | + | <td><a href="http://parts.igem.org/Part:BBa_K2794001">BBa_K2794001</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
<td>CD63 Varient1(Homo sapiens)</td> | <td>CD63 Varient1(Homo sapiens)</td> | ||
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| − | <td><a href="http://parts.igem.org/Part: | + | <td><a href="http://parts.igem.org/Part:BBa_K2794002">BBa_K2794002</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
<td>Nedd4 Family Interacting Protein1 (Ndfip)</td> | <td>Nedd4 Family Interacting Protein1 (Ndfip)</td> | ||
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| − | <td><a href="http://parts.igem.org/Part: | + | <td><a href="http://parts.igem.org/Part:BBa_K2794003">BBa_K2794003</a></td> |
<td>Tag</td> | <td>Tag</td> | ||
<td>WW Domain3</td> | <td>WW Domain3</td> | ||
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| − | <td><a href="http://parts.igem.org/Part: | + | <td><a href="http://parts.igem.org/Part:BBa_K2794004">BBa_K2794004</a></td> |
<td>Tag</td> | <td>Tag</td> | ||
<td>WW Domain4</td> | <td>WW Domain4</td> | ||
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</tr> | </tr> | ||
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| − | <td><a href="http://parts.igem.org/Part: | + | <td><a href="http://parts.igem.org/Part:BBa_K2794005">BBa_K2794005</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
<td>CD63-Vhb Fusion Protein</td> | <td>CD63-Vhb Fusion Protein</td> | ||
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</tr> | </tr> | ||
<tr> | <tr> | ||
| − | <td><a href="http://parts.igem.org/Part: | + | <td><a href="http://parts.igem.org/Part:BBa_K2794006">BBa_K2794006</a></td> |
<td>Coding</td> | <td>Coding</td> | ||
<td>WW Domain3-Vhb Fusion Protein</td> | <td>WW Domain3-Vhb Fusion Protein</td> | ||
Latest revision as of 13:56, 17 October 2018
Parts
BBa_K2794001, as a sequence coding for marker protein of exosome, can be used to insert membrane proteins, signal peptide, or regular proteins to the exosomes. BBa_K2794002 is designed to specifically activates ubiquitination. BBa_K2794003 BBa_K2794004 can be used to actively load protein cargo into exosomes, while BBa_K2794005 focus primarily on specifically load vhb into exosomes. BBa_K2794006 attaches a WW domain to vhb, enabling an alternative for bingding vhb to Ndfip.
All parts information are in the parts page.
| Name | Type | Description | Desginer | Length | Usage |
|---|---|---|---|---|---|
| BBa_K2794001 | Coding | CD63 Varient1(Homo sapiens) | Luo Yan | 717 | Marker protein for exosomes |
| BBa_K2794002 | Coding | Nedd4 Family Interacting Protein1 (Ndfip) | Luo Yan | 663 | Activates E3 Ubiquitines(NEDD4) |
| BBa_K2794003 | Tag | WW Domain3 | Luo Yan | 147 | Mediates interactions between Ndfip and Nedd4 |
| BBa_K2794004 | Tag | WW Domain4 | Luo Yan | 147 | Mediates interactions between Ndfip and Nedd4 |
| BBa_K2794005 | Coding | CD63-Vhb Fusion Protein | Luo Yan | 1227 | Makes exosomes wrapping hemoglobin inside |
| BBa_K2794006 | Coding | WW Domain3-Vhb Fusion Protein | Luo Yan | 654 | Enable hemoglobin to bind with Ndfip |
| BBa_K2794010 | Coding | CD63 Varient1 without stop condon | Luo Yan | 711 | Intermediate serving for fusion protein |
| BBa_K2794999 | Scar | pRK7 SHSU_China Fusion Protein Scar | Luo Yan | 12 | Provides two Xba1 restriction sites for fusion |