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Revision as of 14:13, 17 October 2018
The main focus of our project was to grow lactic acid bacteria and yeast together. We came up with two main specific applications: use in the brewing industry and increase carbon flux to the production of lactic acid to make PLA. Each of these uses brought its own set of questions that we tried to answer over the summer:
Would brewers be comfortable making and selling this co-culture of LAB and yeast?
How do you get approval, nationally and internationally, for a genetically modified organism?
Is it possible to release a genetically modified organism into the wild?
Are each of these applications feasible and important?
We talked to brewers about their experience brewing with multiple species of yeast and if they would sell it. A brewer, Matt Riggs, stated that consumers will be wary of it, so he wouldn't sell it right now, but he personally would try it. He suggested targeting industrial fields in the short-term and the brewing field in the future. Taking his comments and suggestions we geared our project/co-culture toward industrial fields, like using it to create valuable products such as lactic acid. Also, at the CABBI annual retreat we talked to professors working on energy conversion. They gave us suggestions on how to improve the production of these products, such growing LAB in non-aerated conditions as well different combinations of media, which we implemented into our project directly. We started growing our cultures in non-aerated condition (100 RPM) and used a combination of YPD and M17 instead of just one type of media.
Q1: Do you have any experience brewing with multiple species of yeast or non-yeast organisms?
A1: When I was studying brewing in Germany, we sometimes used to use lactobacillus to make sour beers, but this was not a direct co-culture like what you are doing in your lab. We would create a separate culture of lactobacillus and add it to the fermenter in the last 15 minutes of the fermentation, immediately followed by pasterization. The amount of alcohol in the fermentation culture at that point was too high for the lactobacillus to survive, but the lactic acid they already produced was sufficient to give the beer the sour flavour we were looking for.
Q2: What is your opinion on brewing with GMO's?
A2: I should be careful about how I say this. I have heard of people doing some interesting things with GM brews, but I would never mess with that. I would lose most of my business. The fact of the matter is, people don't trust GM products to that extent. If I tried to sell that, I would lose almost all of my business. I think the technology you are working with could do a lot of good in industrial applications like cellulosic ethanol; but I don't think it is viable for something people directly consume.
Q3: Do you think there will ever be a market for specialty brewing with GM organisms?
A3: No, I don't.
Pictures from when we presented our work at the CABBI annual retreat where we had active dialogue with professionals in the research community. We discussed about iGEM requirements, RFC 10 standard, and even synthetic biology as a whole. The research professionals who stopped by our poster gave us advice on how to improve our project to get better results.