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<h1>Safety Protocols and Precautions</h1> | <h1>Safety Protocols and Precautions</h1> | ||
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<ul> | <ul> | ||
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− | <h1> | + | <h1>Project Specific Laboratory Safety</h1> |
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<ins>We also utilized some compounds that required additional safety protocols:</ins> | <ins>We also utilized some compounds that required additional safety protocols:</ins> | ||
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<p><strong>Vaginolysin and human blood</strong>: While creating SynDrop cell-free system special attention was paid to the safety regulations as human-specific toxin vaginolysin was implemented into our system. Vaginolysin is the human-specific cytotoxin produced by Gardnerella vaginalis and believed to play a critical role in the pathogenesis of bacterial vaginosis and its associated morbidities. In order to check vaginolysin activity experiments with human blood was essential. To ensure that it compiles with safety guidelines we managed to get safety form by iGEM Safety Committee and agreement to use it for experiments with human blood from Vilnius University.</p> | <p><strong>Vaginolysin and human blood</strong>: While creating SynDrop cell-free system special attention was paid to the safety regulations as human-specific toxin vaginolysin was implemented into our system. Vaginolysin is the human-specific cytotoxin produced by Gardnerella vaginalis and believed to play a critical role in the pathogenesis of bacterial vaginosis and its associated morbidities. In order to check vaginolysin activity experiments with human blood was essential. To ensure that it compiles with safety guidelines we managed to get safety form by iGEM Safety Committee and agreement to use it for experiments with human blood from Vilnius University.</p> | ||
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− | < | + | <h1>Improved Laboratory Safety</h1> |
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<p>The main idea of SynDrop project was to create a cell-free system for protein research. This system shows potential to be used for more diverse experiments in the lab. As we managed to develop liposomes which can encapsulate IVTT system with required genetic material, one can perform desired research by reducing work with genetically modified organisms. It is advantageous as experiments can be performed without using additional antibiotics. Also, the risk to spread genetically modified organisms diminishes greatly. | <p>The main idea of SynDrop project was to create a cell-free system for protein research. This system shows potential to be used for more diverse experiments in the lab. As we managed to develop liposomes which can encapsulate IVTT system with required genetic material, one can perform desired research by reducing work with genetically modified organisms. It is advantageous as experiments can be performed without using additional antibiotics. Also, the risk to spread genetically modified organisms diminishes greatly. | ||
Revision as of 16:50, 17 October 2018
Safety
Abstract
During the SynDrop creation we took the requirements of the lab work and project very strictly as good laboratory procedures are one of the main principles of our team. In order to this, we managed to further improve the safety in our laboratory environment and suggested platform to reduce some undesired compounds. It is worth to be mentioned that application of our system system can diminish overuse of antibiotics in the daily lab practice. If you are interested how can our team improve laboratory safety – read further.