Difference between revisions of "Team:Kyoto/Demonstrate"
| Line 98: | Line 98: | ||
</ul> | </ul> | ||
</div> | </div> | ||
| − | <p> | + | |
| + | <h5 id="Preparation of salt resistance enhancing plasmid in budding yeast">1) Preparation of salt resistance enhancing plasmid in budding yeast</h5> | ||
| + | <p>We thought that special care would be needed to create cells that absorb Na + as a result of incorporating Na +, so that cells are not damaged. We searched the literature and found mangrin, a chaperone protein isolated from mangrove, cloned it and used it. As written on the link, Salinity tolerance was imparted by expressing mangrin in ENA1Δ strain sensitive to NaCl to take Na +. In addition, mild salinity tolerance was also observed with other plasmids, for example ZrGPD1 for producing compatible solutes glycerol and Avp1, AtNHXS1, SseNHX1 for transporting Na + to vacuoles. As described above, in this study, we developed a parts collection that imparts salt tolerance to budding yeast by several different routes.</p> | ||
| + | |||
| + | <h5 id="Preparation of yeast to incorporate Na +">2) Preparation of yeast to incorporate Na +</h5> | ||
| + | <p>We created a mutant yeast in which all of NHA 1 and ENA 1 - 2 - 5, which drain Na +, knocked out by homologous recombination, and it was actually experimented that this strain stored Na + in cells very efficiently I did (link). Furthermore, the expression of Zvp1, AtNHXS1 or SseNHX1 etc. showed that Na + concentration in the cells increased.</p> | ||
| + | |||
| + | |||
</body> | </body> | ||
Revision as of 17:55, 17 October 2018
1) Preparation of salt resistance enhancing plasmid in budding yeast
We thought that special care would be needed to create cells that absorb Na + as a result of incorporating Na +, so that cells are not damaged. We searched the literature and found mangrin, a chaperone protein isolated from mangrove, cloned it and used it. As written on the link, Salinity tolerance was imparted by expressing mangrin in ENA1Δ strain sensitive to NaCl to take Na +. In addition, mild salinity tolerance was also observed with other plasmids, for example ZrGPD1 for producing compatible solutes glycerol and Avp1, AtNHXS1, SseNHX1 for transporting Na + to vacuoles. As described above, in this study, we developed a parts collection that imparts salt tolerance to budding yeast by several different routes.
2) Preparation of yeast to incorporate Na +
We created a mutant yeast in which all of NHA 1 and ENA 1 - 2 - 5, which drain Na +, knocked out by homologous recombination, and it was actually experimented that this strain stored Na + in cells very efficiently I did (link). Furthermore, the expression of Zvp1, AtNHXS1 or SseNHX1 etc. showed that Na + concentration in the cells increased.