Difference between revisions of "Team:Kyoto/SpecialMethods"

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<h5 id="Boil Method"><font face="Segoe UI">1)Boil Method</font></h2>
 
<h5 id="Boil Method"><font face="Segoe UI">1)Boil Method</font></h2>
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<p>
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<p><font size="10px"><br>
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1.Monitor OD of yeast and incubate until it becomes OD≒1.
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<br>1.Monitor OD of yeast and incubate until it becomes OD≒1.
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2.Centrifuge yeast at 3500 rpm for 5 min  
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<br>2.Centrifuge yeast at 3500 rpm for 5 min  
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3.Discard the supernatant  
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<br>3.Discard the supernatant  
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4.Pipette the culture residual substance and transfer 1 ml of it to an Eppendorf tube
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<br>4.Pipette the culture residual substance and transfer 1 ml of it to an Eppendorf tube
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5.Centrifuge on FLASH and then decantate it
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<br>5.Centrifuge on FLASH and then decantate it
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6.Add 1 ml of distilled water  
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<br>6.Add 1 ml of distilled water  
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7.Vortex
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<br>7.Vortex
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8.Centrifuge yeast at FLASH
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<br>8.Centrifuge yeast at FLASH
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9.Discard the supernatant (wash 1st time)
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<br>9.Discard the supernatant (wash 1st time)
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10.Repeat steps 6-9 (wash 2nd)
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<br>10.Repeat steps 6-9 (wash 2nd)
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11.Repeat steps 6-9 (wash 3rd)
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<br>11.Repeat steps 6-9 (wash 3rd)
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12.Cryopreservation
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<br>12.Cryopreservation
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13.Add 1 ml of distilled water and vortex
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<br>13.Add 1 ml of distilled water and vortex
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14.Boil it with hot water for 10 min  
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<br>14.Boil it with hot water for 10 min  
 
· Fit the tube in the sponge and put it in boiling water  
 
· Fit the tube in the sponge and put it in boiling water  
 
(· Keep the fire between low to medium heat)  
 
(· Keep the fire between low to medium heat)  
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15.Centrifuge at 10000 rpm for 5 min, and remove 900μl of the supernatant  
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<br>15.Centrifuge at 10000 rpm for 5 min, and remove 900μl of the supernatant  
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16.Measure Na+ concentration(Atomic Absorption Spectrometry)  
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<br>16.Measure Na+ concentration(Atomic Absorption Spectrometry)
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</p>
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<br></font>
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<img src="https://2018.igem.org"><p>picture here</p>
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<br></p>
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<br><img src="https://2018.igem.org"><p>picture here</p>
 
<br><br><br>
 
<br><br><br>
 
<h5 id=""><font face="Segoe UI">2)</font></h5>
 
<h5 id=""><font face="Segoe UI">2)</font></h5>

Revision as of 18:01, 17 October 2018

Team:Kyoto/Project - 2018.igem.org

Table of contents
1)Boil Method



1.Monitor OD of yeast and incubate until it becomes OD≒1.
2.Centrifuge yeast at 3500 rpm for 5 min
3.Discard the supernatant
4.Pipette the culture residual substance and transfer 1 ml of it to an Eppendorf tube
5.Centrifuge on FLASH and then decantate it
6.Add 1 ml of distilled water
7.Vortex
8.Centrifuge yeast at FLASH
9.Discard the supernatant (wash 1st time)
10.Repeat steps 6-9 (wash 2nd)
11.Repeat steps 6-9 (wash 3rd)
12.Cryopreservation
13.Add 1 ml of distilled water and vortex
14.Boil it with hot water for 10 min · Fit the tube in the sponge and put it in boiling water (· Keep the fire between low to medium heat)
15.Centrifuge at 10000 rpm for 5 min, and remove 900μl of the supernatant
16.Measure Na+ concentration(Atomic Absorption Spectrometry)


picture here




2)

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3)

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4)


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