Difference between revisions of "Team:Kyoto/SpecialMethods"

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· Fit the tube in the sponge and put it in boiling water  
 
· Fit the tube in the sponge and put it in boiling water  
 
(· Keep the fire between low to medium heat)  
 
(· Keep the fire between low to medium heat)  
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<br>15.Centrifuge at 10000 rpm for 5 min, and remove 900μl of the supernatant  
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<li>15.Centrifuge at 10000 rpm for 5 min, and remove 900μl of the supernatant </li>
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<br>16.Measure Na+ concentration(Atomic Absorption Spectrometry)
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<li>16.Measure Na+ concentration(Atomic Absorption Spectrometry)</li>
 
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<br><img src="https://2018.igem.org"><p>picture here</p>
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Revision as of 18:04, 17 October 2018

Team:Kyoto/Project - 2018.igem.org

Table of contents
1)Boil Method

1.Monitor OD of yeast and incubate until it becomes OD≒1.
2.Centrifuge yeast at 3500 rpm for 5 min
3.Discard the supernatant
4.Pipette the culture residual substance and transfer 1 ml of it to an Eppendorf tube
5.Centrifuge on FLASH and then decantate it
6.Add 1 ml of distilled water
7.Vortex
8.Centrifuge yeast at FLASH
9.Discard the supernatant (wash 1st time)
10.Repeat steps 6-9 (wash 2nd)
11.Repeat steps 6-9 (wash 3rd)
12.Cryopreservation
13.Add 1 ml of distilled water and vortex
14.Boil it with hot water for 10 min · Fit the tube in the sponge and put it in boiling water (· Keep the fire between low to medium heat)

  • 15.Centrifuge at 10000 rpm for 5 min, and remove 900μl of the supernatant
  • 16.Measure Na+ concentration(Atomic Absorption Spectrometry)

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