Difference between revisions of "Team:Kyoto/SpecialMethods"
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<br>13.Add 1 ml of distilled water and vortex | <br>13.Add 1 ml of distilled water and vortex | ||
<br>14.Boil it with hot water for 10 min | <br>14.Boil it with hot water for 10 min | ||
| − | · Fit the tube in the sponge and put it in boiling water | + | <br>· Fit the tube in the sponge and put it in boiling water |
| − | (· Keep the fire between low to medium heat) | + | <br>(· Keep the fire between low to medium heat) |
| − | < | + | <br>15.Centrifuge at 10000 rpm for 5 min, and remove 900μl of the supernatant |
| − | < | + | <br>16.Measure Na+ concentration(Atomic Absorption Spectrometry) |
<br></font> | <br></font> | ||
<br></p> | <br></p> | ||
Revision as of 18:05, 17 October 2018
1)Boil Method
1.Monitor OD of yeast and incubate until it becomes OD≒1.
2.Centrifuge yeast at 3500 rpm for 5 min
3.Discard the supernatant
4.Pipette the culture residual substance and transfer 1 ml of it to an Eppendorf tube
5.Centrifuge on FLASH and then decantate it
6.Add 1 ml of distilled water
7.Vortex
8.Centrifuge yeast at FLASH
9.Discard the supernatant (wash 1st time)
10.Repeat steps 6-9 (wash 2nd)
11.Repeat steps 6-9 (wash 3rd)
12.Cryopreservation
13.Add 1 ml of distilled water and vortex
14.Boil it with hot water for 10 min
· Fit the tube in the sponge and put it in boiling water
(· Keep the fire between low to medium heat)
15.Centrifuge at 10000 rpm for 5 min, and remove 900μl of the supernatant
16.Measure Na+ concentration(Atomic Absorption Spectrometry)
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