Difference between revisions of "Team:Kyoto/Result"
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Revision as of 18:07, 17 October 2018
In starting the experiment, we conducted an experiment demonstrating how the salt concentration of the solution actually affects the protein-protein interaction by using the model protein. In this example, we chose GFP BBa_E0040, which is commonly used in many experiments as a model protein. TDH3 promoter and CYC1 terminator were added to both ends of ORF and cloned into pRS316 which is a shuttle vector of S. cerevisiae and E. coli. The resulting plasmid was transformed into wild-type yeast strain BY 4741 to overexpress GFP in yeast. As a comparative experiment, yeast expressing RFP (BBa - E 0010) with the same set of promoter and terminator was used.
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Reference
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- [5] V. W. Pollard and M. H. Malim, “the Hiv-1 Rev Protein,” Annu. Rev. Microbiol., vol. 52, no. 1, pp. 491–532, 1998.