Difference between revisions of "Team:TU-Eindhoven/Protocols"

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<h1 id="protocols">Protocols</h1>
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<p>Below, the protocols we’ve used during our labwork are listed. They can be downloaded in pdf format.</p>
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<h2 id="general-protocols">General protocols </h2>
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        <h1 id="protocols">Protocols</h1>
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<p><a href="https://static.igem.org/mediawiki/2018/6/60/T--TU-Eindhoven--TU_Eindhoven_Protocols_Preparation_of_general_necessities.pdf">Preparation general necessities</a></p>
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        <p>Below, the protocols we’ve used during our labwork are listed. They can be downloaded in pdf format.</p>
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<p><a href="https://static.igem.org/mediawiki/2018/1/14/T--TU-Eindhoven--TU_Eindhoven_Protocols_Glycerol_Stock.pdf">Glycerol stock</a></p>
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        <h2 id="general-protocols">General protocols </h2>
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<p><a href="https://static.igem.org/mediawiki/2018/e/e3/T--TU-Eindhoven--TU_Eindhoven_Protocols_Streaking_Glycerol_Stock.pdf">Streaking glycerol stock</a></p>
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        <p><a href="https://static.igem.org/mediawiki/2018/6/60/T--TU-Eindhoven--TU_Eindhoven_Protocols_Preparation_of_general_necessities.pdf">Preparation general necessities</a></p>
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<p><a href="https://static.igem.org/mediawiki/2018/1/15/T--TU-Eindhoven--TU_Eindhoven_Protocols_NanoDrop.pdf">Nanodrop</a></p>
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        <p><a href="https://static.igem.org/mediawiki/2018/1/14/T--TU-Eindhoven--TU_Eindhoven_Protocols_Glycerol_Stock.pdf">Glycerol stock</a></p>
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<p><a href="https://static.igem.org/mediawiki/2018/9/93/T--TU-Eindhoven--TU_Eindhoven_Protocols_Agarose_Gel_Electrophoresis.pdf">Agarose gel electrophoresis</a></p>
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        <p><a href="https://static.igem.org/mediawiki/2018/e/e3/T--TU-Eindhoven--TU_Eindhoven_Protocols_Streaking_Glycerol_Stock.pdf">Streaking glycerol stock</a></p>
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<p><a href="https://static.igem.org/mediawiki/2018/9/98/T--TU-Eindhoven--TU_Eindhoven_Protocols_SDS-Page_Analysis.pdf">SDS-Page</a></p>
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        <p><a href="https://static.igem.org/mediawiki/2018/1/15/T--TU-Eindhoven--TU_Eindhoven_Protocols_NanoDrop.pdf">Nanodrop</a></p>
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<h2 id="cloning-and-biobricking">Cloning and BioBricking </h2>
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        <p><a href="https://static.igem.org/mediawiki/2018/9/93/T--TU-Eindhoven--TU_Eindhoven_Protocols_Agarose_Gel_Electrophoresis.pdf">Agarose gel electrophoresis</a></p>
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<p><a href="https://static.igem.org/mediawiki/2018/c/c1/T--TU-Eindhoven--TU_Eindhoven_Protocols_PCR_Amplification.pdf">PCR Amplification</a></p>
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        <p><a href="https://static.igem.org/mediawiki/2018/9/98/T--TU-Eindhoven--TU_Eindhoven_Protocols_SDS-Page_Analysis.pdf">SDS-Page</a></p>
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<p><a href="https://static.igem.org/mediawiki/2018/5/52/T--TU-Eindhoven--TU_Eindhoven_Protocols_Digestion.pdf">Digestion</a></p>
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        <h2 id="cloning-and-biobricking">Cloning and BioBricking </h2>
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<p><a href="https://static.igem.org/mediawiki/2018/5/57/T--TU-Eindhoven--TU_Eindhoven_Protocols_Ligation.pdf">Ligation</a></p>
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        <p><a href="https://static.igem.org/mediawiki/2018/c/c1/T--TU-Eindhoven--TU_Eindhoven_Protocols_PCR_Amplification.pdf">PCR Amplification</a></p>
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<p><a href="https://static.igem.org/mediawiki/2018/2/2a/T--TU-Eindhoven--TU_Eindhoven_Protocols_Vector_Linearization.pdf">Vector linearization</a></p>
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        <p><a href="https://static.igem.org/mediawiki/2018/5/52/T--TU-Eindhoven--TU_Eindhoven_Protocols_Digestion.pdf">Digestion</a></p>
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<p><a href="https://static.igem.org/mediawiki/2018/3/38/T--TU-Eindhoven--TU_Eindhoven_Protocols_Gibson_assembly.pdf">Gibson assembly</a></p>
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        <p><a href="https://static.igem.org/mediawiki/2018/5/57/T--TU-Eindhoven--TU_Eindhoven_Protocols_Ligation.pdf">Ligation</a></p>
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<p><a href="https://static.igem.org/mediawiki/2018/4/48/T--TU-Eindhoven--TU_Eindhoven_Protocols_Gel_purification.pdf">Gel purification</a></p>
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        <p><a href="https://static.igem.org/mediawiki/2018/2/2a/T--TU-Eindhoven--TU_Eindhoven_Protocols_Vector_Linearization.pdf">Vector linearization</a></p>
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<h2 id="transformation-and-protein-expression">Transformation and protein expression</h2>
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        <p><a href="https://static.igem.org/mediawiki/2018/3/38/T--TU-Eindhoven--TU_Eindhoven_Protocols_Gibson_assembly.pdf">Gibson assembly</a></p>
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<p><a href="https://static.igem.org/mediawiki/2018/b/ba/T--TU-Eindhoven--TU_Eindhoven_Protocols_Transformation_Into_Competent_Cells.pdf">Transformation into competent cells</a></p>
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        <p><a href="https://static.igem.org/mediawiki/2018/4/48/T--TU-Eindhoven--TU_Eindhoven_Protocols_Gel_purification.pdf">Gel purification</a></p>
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<p><a href="https://static.igem.org/mediawiki/2018/f/f8/T--TU-Eindhoven--TU_Eindhoven_Protocols_Plating.pdf">Plating</a></p>
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        <h2 id="transformation-and-protein-expression">Transformation and protein expression</h2>
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<p><a href="https://static.igem.org/mediawiki/2018/2/2b/T--TU-Eindhoven--TU_Eindhoven_Protocols_Colony_Picking_Colony_PCR.pdf">Colony picking and Colony PCR</a></p>
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        <p><a href="https://static.igem.org/mediawiki/2018/b/ba/T--TU-Eindhoven--TU_Eindhoven_Protocols_Transformation_Into_Competent_Cells.pdf">Transformation into competent cells</a></p>
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<p><a href="https://static.igem.org/mediawiki/2018/7/7f/T--TU-Eindhoven--TU_Eindhoven_Protocols_Preparing_BaseClear_Sequencing_Samples.pdf">Sequencing at BaseClear</a></p>
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        <p><a href="https://static.igem.org/mediawiki/2018/f/f8/T--TU-Eindhoven--TU_Eindhoven_Protocols_Plating.pdf">Plating</a></p>
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<p><a href="https://static.igem.org/mediawiki/2018/8/82/T--TU-Eindhoven--TU_Eindhoven_Protocols_Miniprep.pdf">Miniprep</a></p>
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        <p><a href="https://static.igem.org/mediawiki/2018/2/2b/T--TU-Eindhoven--TU_Eindhoven_Protocols_Colony_Picking_Colony_PCR.pdf">Colony picking and Colony PCR</a></p>
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<p><a href="https://static.igem.org/mediawiki/2018/b/b7/T--TU-Eindhoven--TU_Eindhoven_Protocols_Small_Culturing.pdf">Small culturing</a></p>
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        <p><a href="https://static.igem.org/mediawiki/2018/7/7f/T--TU-Eindhoven--TU_Eindhoven_Protocols_Preparing_BaseClear_Sequencing_Samples.pdf">Sequencing at BaseClear</a></p>
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<h2 id="project-specific-protocols">Project specific protocols</h2>
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        <p><a href="https://static.igem.org/mediawiki/2018/8/82/T--TU-Eindhoven--TU_Eindhoven_Protocols_Miniprep.pdf">Miniprep</a></p>
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<p><a href="https://static.igem.org/mediawiki/2018/5/52/T--TU-Eindhoven--TU_Eindhoven_Protocols_IBP_protocols.pdf">Hydrogel seeding experiments</a></p>
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        <p><a href="https://static.igem.org/mediawiki/2018/b/b7/T--TU-Eindhoven--TU_Eindhoven_Protocols_Small_Culturing.pdf">Small culturing</a></p>
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        <h2 id="project-specific-protocols">Project specific protocols</h2>
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        <p><a href="https://static.igem.org/mediawiki/2018/5/52/T--TU-Eindhoven--TU_Eindhoven_Protocols_IBP_protocols.pdf">Hydrogel seeding experiments</a></p>
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Revision as of 18:38, 17 October 2018

Protocols

Below, the protocols we’ve used during our labwork are listed. They can be downloaded in pdf format.

General protocols

Preparation general necessities

Glycerol stock

Streaking glycerol stock

Nanodrop

Agarose gel electrophoresis

SDS-Page

Cloning and BioBricking

PCR Amplification

Digestion

Ligation

Vector linearization

Gibson assembly

Gel purification

Transformation and protein expression

Transformation into competent cells

Plating

Colony picking and Colony PCR

Sequencing at BaseClear

Miniprep

Small culturing

Project specific protocols

Hydrogel seeding experiments