Difference between revisions of "Team:SCUT-ChinaA/Parts"

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<h2>Parts</h2>
 
<h2>Parts</h2>
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<p>Each team will make new parts during iGEM and will submit them to the Registry of Standard Biological Parts. The iGEM software provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
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<p style="text-align: justify">We standardized some integration modules involved in our project. The sequences of our integration modules have been codon-optimized for the heterologously expression in <em>Yarrowia lipolytica</em> and are able to successfully transcribe. The standardized modules we build can be used with each other. The two terminators provided have been verified that can stop the transcriptional activity of the transcription complex effectively, and can be used widely in <em> Y. lipolytica.</em> The part SpyCatcher can be fused with other enzyme involved in the metabolic pathway. Thus together with the SpyTag, by constructing metabolic coupling modules thereby enhancing the metabolic flux, In theory, the inhibition of any rate-limiting step in the pathway can be released. SpyTag-Ls is an assembly module successfully built in our project. Tag and LS are connected by PT linker. The part tHMG1 is recognized as a rate-limiting enzyme in the MVA pathway, and increasing its copy number can definitely enhance the metabolic flux of MVA.</p>
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
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<h3>Note</h3>
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<p>Note that parts must be documented on the <a href="http://parts.igem.org/Main_Page"> Registry</a>. This page serves to <i>showcase</i> the parts you have made. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<th> Name</th> <th>Type</th> <th>Description</th> <th>Length</th> <th>Code</th>
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<td> XPR2</td> <td>Terminator</td> <td>XPR2</td> <td>411</td> <td>BBa_K2733001</td> </tr>
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<td> lip2</td> <td>Terminator</td> <td>lip2</td> <td>202</td> <td>BBa_K2733002</td> </tr>
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<td> LS</td> <td>Coding</td> <td>limonene synthase from Agastache rugosa</td> <td>1842</td> <td>BBa_K2733003</td> </tr>
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<td> SpyCatcher</td> <td>Coding</td> <td>SpyCatcher</td> <td>417</td> <td>BBa_K2733004</td> </tr>
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<td> SpyTag-LS</td> <td>Composite</td> <td>SpyTag-LS</td> <td>1932</td> <td>BBa_K2733005</td> </tr>
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<td> tHMG1</td> <td>Coding</td> <td>tHMG1</td> <td>1503</td> <td>BBa_K2733006</td> </tr>
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<h3>Adding parts to the registry</h3>
 
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
 
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Remember, you don't need to send us the DNA sample before you create an entry for a part on the Registry. (However, you <b>do</b> need to send us the DNA sample before the Jamboree. If you don't send us a DNA sample of a part, that part will not be eligible for awards and medal criteria.)</p>
 
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<a href="http://parts.igem.org/Add_a_Part_to_the_Registry">
 
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ADD PARTS
 
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<h3>Inspiration</h3>
 
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
 
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
 
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
 
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
 
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
 
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<h3>What information do I need to start putting my parts on the Registry?</h3>
 
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<p>The information needed to initially create a part on the Registry is:</p>
 
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<li>Part Name</li>
 
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<li>Part type</li>
 
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<li>Creator</li>
 
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<li>Sequence</li>
 
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<li>Short Description (60 characters on what the DNA does)</li>
 
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<li>Long Description (Longer description of what the DNA does)</li>
 
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<li>Design considerations</li>
 
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, please also put it up on the part page. </p>
 
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<h3>Part Table </h3>
 
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
 
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<groupparts>iGEM18 SCUT-ChinaA</groupparts>
 
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Revision as of 19:39, 17 October 2018

SCUT-ChinaA

Parts

We standardized some integration modules involved in our project. The sequences of our integration modules have been codon-optimized for the heterologously expression in Yarrowia lipolytica and are able to successfully transcribe. The standardized modules we build can be used with each other. The two terminators provided have been verified that can stop the transcriptional activity of the transcription complex effectively, and can be used widely in Y. lipolytica. The part SpyCatcher can be fused with other enzyme involved in the metabolic pathway. Thus together with the SpyTag, by constructing metabolic coupling modules thereby enhancing the metabolic flux, In theory, the inhibition of any rate-limiting step in the pathway can be released. SpyTag-Ls is an assembly module successfully built in our project. Tag and LS are connected by PT linker. The part tHMG1 is recognized as a rate-limiting enzyme in the MVA pathway, and increasing its copy number can definitely enhance the metabolic flux of MVA.

Name Type Description Length Code
XPR2 Terminator XPR2 411 BBa_K2733001
lip2 Terminator lip2 202 BBa_K2733002
LS Coding limonene synthase from Agastache rugosa 1842 BBa_K2733003
SpyCatcher Coding SpyCatcher 417 BBa_K2733004
SpyTag-LS Composite SpyTag-LS 1932 BBa_K2733005
tHMG1 Coding tHMG1 1503 BBa_K2733006