Difference between revisions of "Team:US AFRL CarrollHS/Demonstrate"
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| − | + | <div class="row"><p>To determine the efficacy of our destroy mechanism, we decided to test the ability of cinnamaldehyde and chitinase to inhibit the growth of Yarrowia lipolytica. We used Yarrowia lipolytica for our testing because it was one of the fungal isolates from fuel tanks that Dr. Wendy Goodson provided us. For our experiment, we plated dilute fungi culture on TSA plates, let them dry, and then pipetted spots of cinnamaldehyde, chitinase, and a combination of both onto the plates (the full protocol for our testing can be found on our Experiments page). After letting them incubate at 27°C for 1-2 days, we examined the plates for zones of clearing. Plates from one of our tests are shown below.</p> | |
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| + | <div class="col-sm-6 text-center"><img src="https://static.igem.org/mediawiki/2018/8/8c/T--US_AFRL_CarrollHS--ChitinaseTest.jpg" style="width:90%"></div> | ||
| + | <div class="col-sm-6 text-center"><img src="https://static.igem.org/mediawiki/2018/5/59/T--US_AFRL_CarrollHS--CinnaTest.jpg" width="90%"></div> | ||
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| + | <div class="col-sm-6 label"><p class="text-center">Figure 1. Chitinase test plate. The concentration of chitinase for the top row is 10 mg/ml, 1 mg/ml for the middle row, and 0.1 mg/ml for the bottom.</p></div> | ||
| + | <div class="col-sm-6 label"><p class="text-center">Figure 2. Cinnamaldehyde test plate. The concentration of cinnamaldehyde for the top row is 0.66 mg/ml, 0.33 mg/ml for the middle row, and 0.16 mg/ml for the bottom.</p></div> | ||
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| − | + | <div class="col-sm-12 text-center"><img src="https://static.igem.org/mediawiki/2018/6/64/T--US_AFRL_CarrollHS--CombinedTest.jpg" style="width:60%"></div> | |
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| + | <div class="col-sm-12 label"><p class="text-center">Figure 3. Combined test plate. The concentration of chitinase and cinnamaldehyde respectively for the top row is 10 mg/ml and 0.66 mg/ml, 10 mg/ml and 0.33 mg/ml for the second row, 1 mg/ml and 0.66 mg/ml mg/ml for the third row, and 1 mg/ml and 0.33 mg/ml for the bottom row.</p></div> | ||
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| + | <div class="row"><p>To determine the efficacy of our destroy mechanism, we decided to test the ability of cinnamaldehyde and chitinase to inhibit the growth of Yarrowia lipolytica. We used Yarrowia lipolytica for our testing because it was one of the fungal isolates from fuel tanks that Dr. Wendy Goodson provided us. For our experiment, we plated dilute fungi culture on TSA plates, let them dry, and then pipetted spots of cinnamaldehyde, chitinase, and a combination of both onto the plates (the full protocol for our testing can be found on our Experiments page). After letting them incubate at 27°C for 1-2 days, we examined the plates for zones of clearing. Plates from one of our tests are shown below.</p> | ||
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Revision as of 20:30, 17 October 2018
To determine the efficacy of our destroy mechanism, we decided to test the ability of cinnamaldehyde and chitinase to inhibit the growth of Yarrowia lipolytica. We used Yarrowia lipolytica for our testing because it was one of the fungal isolates from fuel tanks that Dr. Wendy Goodson provided us. For our experiment, we plated dilute fungi culture on TSA plates, let them dry, and then pipetted spots of cinnamaldehyde, chitinase, and a combination of both onto the plates (the full protocol for our testing can be found on our Experiments page). After letting them incubate at 27°C for 1-2 days, we examined the plates for zones of clearing. Plates from one of our tests are shown below.
Figure 1. Chitinase test plate. The concentration of chitinase for the top row is 10 mg/ml, 1 mg/ml for the middle row, and 0.1 mg/ml for the bottom.
Figure 2. Cinnamaldehyde test plate. The concentration of cinnamaldehyde for the top row is 0.66 mg/ml, 0.33 mg/ml for the middle row, and 0.16 mg/ml for the bottom.
Figure 3. Combined test plate. The concentration of chitinase and cinnamaldehyde respectively for the top row is 10 mg/ml and 0.66 mg/ml, 10 mg/ml and 0.33 mg/ml for the second row, 1 mg/ml and 0.66 mg/ml mg/ml for the third row, and 1 mg/ml and 0.33 mg/ml for the bottom row.
To determine the efficacy of our destroy mechanism, we decided to test the ability of cinnamaldehyde and chitinase to inhibit the growth of Yarrowia lipolytica. We used Yarrowia lipolytica for our testing because it was one of the fungal isolates from fuel tanks that Dr. Wendy Goodson provided us. For our experiment, we plated dilute fungi culture on TSA plates, let them dry, and then pipetted spots of cinnamaldehyde, chitinase, and a combination of both onto the plates (the full protocol for our testing can be found on our Experiments page). After letting them incubate at 27°C for 1-2 days, we examined the plates for zones of clearing. Plates from one of our tests are shown below.