Difference between revisions of "Team:Kyoto/SpecialMethods"
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<span class="box-title"><font face="Segoe UI">Table of contents</font></span> | <span class="box-title"><font face="Segoe UI">Table of contents</font></span> | ||
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<li><a href="#Boil Method">1)Boil Method</a></li> | <li><a href="#Boil Method">1)Boil Method</a></li> | ||
| − | + | <li><a href="#"><font color="#fffafa"><font face="Segoe UI">2)</font></a></li> | |
| + | <li><a href="#"><font color="#fffafa"><font face="Segoe UI">3)</font></a></li> | ||
| + | <li><a href="#"><font color="#fffafa"><font face="Segoe UI">4)</font></a></li> | ||
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Revision as of 20:40, 17 October 2018
1)Boil Method
1.Monitor OD of yeast and incubate until it becomes OD≒1.
2.Centrifuge yeast at 3500 rpm for 5 min
3.Discard the supernatant
4.Pipette the culture residual substance and transfer 1 ml of it to an Eppendorf tube
5.Centrifuge on FLASH and then decantate it
6.Add 1 ml of distilled water
7.Vortex
8.Centrifuge yeast at FLASH
9.Discard the supernatant (wash 1st time)
10.Repeat steps 6-9 (wash 2nd)
11.Repeat steps 6-9 (wash 3rd)
12.Cryopreservation
13.Add 1 ml of distilled water and vortex
14.Boil it with hot water for 10 min
· Fit the tube in the sponge and put it in boiling water
(· Keep the fire between low to medium heat)
15.Centrifuge at 10000 rpm for 5 min, and remove 900μl of the supernatant
16.Measure Na+ concentration(Atomic Absorption Spectrometry)
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2)
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3)
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4)
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