Difference between revisions of "Team:Munich/growth.html"
Franziwinzig (Talk | contribs) |
Franziwinzig (Talk | contribs) |
||
| Line 16: | Line 16: | ||
<tr> | <tr> | ||
<td>Notes:</td> | <td>Notes:</td> | ||
| − | <td> | + | <td> Strains tested: deltamsbB; DeltaRecB, DeltaRecD and WT (MG1655); inoculation of 100 ml LB medium with 1 ml preculture. Measure OD every 30 – 60 minutes. |
| + | |||
</td> | </td> | ||
</tr> | </tr> | ||
Revision as of 20:50, 17 October 2018
Growth of msbB and RecBCD-Nuklease deficient E.Coli mutant strains
2018/08/28| Participants: | Katja Neishsalo |
| Protocol: | Measuring optical density |
| Notes: | Strains tested: deltamsbB; DeltaRecB, DeltaRecD and WT (MG1655); inoculation of 100 ml LB medium with 1 ml preculture. Measure OD every 30 – 60 minutes. |
| Results: | 
|
preparing electro-competent cells of WT RecBCD und dRecB
2018/08/31| Participants: | Katja Neishsalo |
| Protocol: | Preparation electrocompetent cells |
| Results: | PICcells are still viable after preparation, dRecB grows significantly slower than others |
Transforming E. Coli dRecB with pSB1C3_RecBCD(WT)
2018/09/03| Participants: | Katja Neishsalo |
| Protocol: | Electrocompetent transformation |
| Results: | Colonies |
DNA midi prep of RecBCD(WT) plasmid from Dh5a
2018/09/03| Participants: | Katja Neishsalo |
| Protocol: | Midiprep |
Growth of RecB, RecD, RecBCD, msb�-B deficient E.Coli strains
2018/09/14| Participants: | Katja Neishsalo |
| Protocol: | Measuring optical density |
| Notes: | |
| Results: | PIC |