Difference between revisions of "Team:ECUST/Biocide"
Universeson (Talk | contribs) |
|||
| Line 27: | Line 27: | ||
</figure> | </figure> | ||
| + | <figure> | ||
| Line 46: | Line 47: | ||
<figcaption><b>Figure 2. 1% Agarose Gel Electrophoresis of PCR, which shows that our vector was successfully constructed.</b></figcaption> | <figcaption><b>Figure 2. 1% Agarose Gel Electrophoresis of PCR, which shows that our vector was successfully constructed.</b></figcaption> | ||
</figure> | </figure> | ||
| + | <figure> | ||
| Line 57: | Line 59: | ||
</b></figcaption> | </b></figcaption> | ||
</figure> | </figure> | ||
| − | + | <figure> | |
Revision as of 21:25, 17 October 2018
Description
When the concentration of Fe3+ in the environment is high, it means that the hard skeleton of the microenvironment of the iron bacteria is destroyed and loses protection. At this time, the added bactericide will have a greater killing effect on the bacteria. The bactericide we selected was cecropin AD, a cationic chimeric antimicrobial peptide obtained from cecropin, consisting of the first 11 amino acid residues of cecropin A and the last 26 amino acid residues of cecropin D. It has strong antibacterial activity against Gram-positive and negative bacteria.
Design
Result
We insert fragment of Cecropin AD into vector pET-28a(Figure 1)
The plasmid was transformed to E. coli BL21 and cultured at 37 °C for 12 h. positive monoclonal bacteria were cultured and verified by PCR(Figure 2).
We verified the expression of Cecropin AD by SDS-PAGE(Figure 3).
