Difference between revisions of "Team:US AFRL CarrollHS"

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<h1 class="right">Our Music Video!</h1>
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<p class="right2">We had a lot of fun working on our iGEM project, including the creation of our music video.</p>
<img src="https://static.igem.org/mediawiki/2018/f/f0/T--US_AFRL_CarrollHS--MikeFixed.png" style="width: 100%;">
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<div class="label" style="position: relative; bottom: 70px"><p class="text-center">Mike the Microbe!</p></div>
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<video width="100%" height="500" controls>  
 
<video width="100%" height="500" controls>  
 
<source src="https://static.igem.org/mediawiki/2018/8/80/T--US_AFRL_CarrollHS--MusicVideo.mp4"type="video/mp4">  
 
<source src="https://static.igem.org/mediawiki/2018/8/80/T--US_AFRL_CarrollHS--MusicVideo.mp4"type="video/mp4">  
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<img src="https://static.igem.org/mediawiki/2018/2/21/T--US_AFRL_CarrollHS--ChiaTheChitinase.png" width="100%">
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<div class="label" style="position: relative; bottom: 70px"><p class="text-center">Chia the Chitinase!</p></div>
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Revision as of 21:41, 17 October 2018

Abstract

With growing environmental concerns, industries are increasingly relying on the use of biofuels. Biodiesel storage tanks are susceptible to water infiltration that often results in the formation of biofilms containing bacteria and fungi. Biofilms may clog pipes, degrade fuel, or corrode storage tanks. We set out to engineer a “seek, aim, and destroy” system for the remediation of microbial biofilm. Pseudomonas aeruginosa, commonly found in fuel biofilms, releases the quorum sensing molecule C4-HSL. Our engineered E. coli cells express CheZ protein in response to a concentration gradient of C4-HSL to activate the flagella motors and propel the cells towards the biofilm. In addition, the engineered E. coli expresses chitinase on its surface and secretes cinnamaldehyde. Chitinase breaks down chitin in the fungal cell walls, increasing the ability of cinnamaldehyde to destroy the fungi. Cinnamaldehyde also eliminates bacteria, thus remediating the biofilm.


Our Approach

We split our project up into three parts.


We split our project up into three parts.


Our Approach

We split our project up into three parts.


Our Approach

We split our project up into three parts.


Our Approach

We split our project up into three parts.


Our Approach

We split our project up into three parts.


Our Music Video!

We had a lot of fun working on our iGEM project, including the creation of our music video.