Line 262: | Line 262: | ||
</table> | </table> | ||
+ | |||
+ | <table> | ||
+ | |||
+ | <tr><th>Number | ||
+ | </th><th width=30%>parts | ||
+ | </th><th>Remark | ||
+ | </th><th width=8%>Designer | ||
+ | </th><th>Experimental data | ||
+ | </th><th>Subimittion | ||
+ | </th></tr> | ||
+ | |||
+ | <tr><td><a href="http://parts.igem.org/Part:BBa_K2665011"><font color="#007081"><b>BBa_K2665011 | ||
+ | </b></font></a></td><td>TDH3-Mangrin-6xHis-CYC | ||
+ | </td><td>- | ||
+ | </td><td>Tong | ||
+ | </td><td>Spot Assay<br> and Boil method | ||
+ | </td><td>"submitted 1A" | ||
+ | |||
+ | </td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665012"><font color="#007081"><b>BBa_K2665012 | ||
+ | </b></font></a></td><td>TDH3-ZrGPD1-6xHis-CYC | ||
+ | </td><td>- | ||
+ | </td><td>Tong | ||
+ | </td><td>〃 | ||
+ | </td><td>"submitted 1B" | ||
+ | |||
+ | </td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665013"><font color="#007081"><b>BBa_K2665013 | ||
+ | </b></font></a></td><td>TDH3-AtNHXS1-6xHis-CYC | ||
+ | </td><td>- | ||
+ | </td><td>Tong | ||
+ | </td><td>〃 | ||
+ | </td><td>"submitted 1C" | ||
+ | |||
+ | </td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665014"><font color="#007081"><b>BBa_K2665014 | ||
+ | </b></font></a></td><td>TDH3-SseNHX1-6xHis-CYC | ||
+ | </td><td>codon optimized | ||
+ | </td><td>Tong | ||
+ | </td><td>〃 | ||
+ | </td><td>"submitted 1E" | ||
+ | |||
+ | </td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665015"><font color="#007081"><b>BBa_K2665015 | ||
+ | </b></font></a></td><td>TDH3-AVP1-6xHis-CYC | ||
+ | </td><td>not excluded the stop codon | ||
+ | </td><td>Tong | ||
+ | </td><td>〃 | ||
+ | </td><td>"submitted 1D" | ||
+ | |||
+ | </td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665016"><font color="#007081"><b>BBa_K2665016 | ||
+ | </b></font></a></td><td>TDH3-AtHKT1-6xHis-CYC | ||
+ | </td><td>- | ||
+ | </td><td>Tong | ||
+ | </td><td>〃 | ||
+ | </td><td>"submitted1F" | ||
+ | |||
+ | </td></tr><tr><td><a href="http://parts.igem.org/Part:BBa_K2665017"><font color="#007081"><b>BBa_K2665017 | ||
+ | </b></font></a></td><td>TDH3-ZrFPS1-6xHis-CYC | ||
+ | </td><td>- | ||
+ | </td><td>Tong | ||
+ | </td><td>〃 | ||
+ | </td><td>not submitted | ||
+ | |||
+ | |||
+ | </td></tr><tr><td><a href=""><font color="#007081"><b>BBa_K2665020 | ||
+ | </b></font></a></td><td>Fgb-stalk | ||
+ | </td><td>- | ||
+ | </td><td>Tong | ||
+ | </td><td>〃 | ||
+ | </td><td>"submitted 2C" | ||
+ | |||
+ | </td></tr><tr><td><a href=""><font color="#007081"><b>BBa_K2665021 | ||
+ | </b></font></a></td><td>SdrG-stalk | ||
+ | </td><td>- | ||
+ | </td><td>Tong | ||
+ | </td><td>〃 | ||
+ | </td><td>not submitted | ||
+ | </td></tr> | ||
+ | |||
+ | |||
+ | |||
+ | |||
+ | |||
+ | </table> | ||
+ | |||
<!-- Table end --> | <!-- Table end --> | ||
Revision as of 21:59, 17 October 2018
This Part Collection enables to create a biological desalination yeast with aggregation system. BBa_K2665018, BBa_K2665019 and BBa_K2665020 work for yeast aggregation. BBa_K2665011 and BBa_K2665012 can be used for raising yeasts' halotorelance, and parts of BBa_K2665013 to BBa_K2665016 enhance yeasts' ability of Na+ uptake. BBa_K2665011 to BBa_K2665016 include a constitutive yeast promoter and a common terminator so iGEMers can use them without assembly to express these proteins in yeast.
Basic Parts
Number | parts | Remark | Designer | Remark2 | Subimittion |
---|---|---|---|---|---|
BBa_K2665000 | TDH3 promoter | - | Tong | - | not submitted |
BBa_K2665001 | CYCterm fused with 6xHis tag and stop codon | - | Tong | - | not submitted |
BBa_K2665002 | Mangrin ORF | - | Tong | - | not submitted |
BBa_K2665003 | ZrGPD1 ORF | - | Tong | - | not submitted |
BBa_K2665004 | AtNHXS1 ORF | - | Tong | - | not submitted |
BBa_K2665005 | SseNHX1 ORF | - | Tong | - | not submitted |
BBa_K2665006 | AVP1 ORF | including the stop codon | Tong | - | not submitted |
BBa_K2665007 | AtHKT1 ORF | - | Tong | - | not submitted |
BBa_K2665008 | ZrFPS1 ORF | - | Tong | - | not submitted |
BBa_K2665018 | Fgb | - | Tong | Fluorescence Microscope | "submitted 2A" |
BBa_K2665019 | SdrG | - | Tong | Fluorescence Microscope | "submitted 2B" |
BBa_K2665022 | Stalk | - | Tong | Fluorescence Microscope | not submitted |
BBa_K2665023 | ybbr-tag | Refer to the following K2665018, K2665019 | Tong | - | not submitted |
BBa_K2665024 | ddFLN4 | Refer to the following K2665018, K2665020 | Tong | - | not submitted |
BBa_K2665025 | SED1 secretion signal | - | Tong | - | not submitted |
BBa_K2665026 | SED1 anchoring domain | - | Tong | - | not submitted |
Number | parts | Remark | Designer | Experimental data | Subimittion |
---|---|---|---|---|---|
BBa_K2665011 | TDH3-Mangrin-6xHis-CYC | - | Tong | Spot Assay and Boil method | "submitted 1A" |
BBa_K2665012 | TDH3-ZrGPD1-6xHis-CYC | - | Tong | 〃 | "submitted 1B" |
BBa_K2665013 | TDH3-AtNHXS1-6xHis-CYC | - | Tong | 〃 | "submitted 1C" |
BBa_K2665014 | TDH3-SseNHX1-6xHis-CYC | codon optimized | Tong | 〃 | "submitted 1E" |
BBa_K2665015 | TDH3-AVP1-6xHis-CYC | not excluded the stop codon | Tong | 〃 | "submitted 1D" |
BBa_K2665016 | TDH3-AtHKT1-6xHis-CYC | - | Tong | 〃 | "submitted1F" |
BBa_K2665017 | TDH3-ZrFPS1-6xHis-CYC | - | Tong | 〃 | not submitted |
BBa_K2665020 | Fgb-stalk | - | Tong | 〃 | "submitted 2C" |
BBa_K2665021 | SdrG-stalk | - | Tong | 〃 | not submitted |