Difference between revisions of "Team:Gifu/Results"
| Line 31: | Line 31: | ||
<h1>Cell-free expression</h1> | <h1>Cell-free expression</h1> | ||
<p>Here we show the result of expression. (Fig. 4~7) Seq.2 (monomer RFP, mRFP) was expressed well in PURE system and slightly expressed in myTXTL. Seq.1 (polymer RFP, pRFP) has no fluorescence. | <p>Here we show the result of expression. (Fig. 4~7) Seq.2 (monomer RFP, mRFP) was expressed well in PURE system and slightly expressed in myTXTL. Seq.1 (polymer RFP, pRFP) has no fluorescence. | ||
| + | <figure> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/c/c4/T--Gifu--BUVPURE.jpg" width="350px"><br> | ||
| + | <center> <legend><b>Fig.4 Expression in PURE system (No UV) </b><p>P: Positive (DHFR gene), N: Negative (Water), tem.: Template DNA</p></legend></center> | ||
</div> | </div> | ||
Revision as of 22:17, 17 October 2018
Results
PCR
The result of PCR is shown in Fig.1. All PCR was performed by primers in table 1. All PCR were succeeded. We used PrimeSTAR Max premix for amplification.
Cell-free expression
Here we show the result of expression. (Fig. 4~7) Seq.2 (monomer RFP, mRFP) was expressed well in PURE system and slightly expressed in myTXTL. Seq.1 (polymer RFP, pRFP) has no fluorescence.
