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width=" | width=" | ||
100%" height="900px"> | 100%" height="900px"> | ||
+ | </object> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="row"> | ||
+ | <div class="col"> | ||
+ | <a class="btn-link text-notebook" data-toggle="collapse" href="#creationpolyacrylamideab" role="button" | ||
+ | aria-expanded="false" aria-controls="creationpolyacrylamideab"> | ||
+ | <h3>Protocol to make polyacrylamide AB</h3> | ||
+ | </a> | ||
+ | <div class="collapse" id="creationpolyacrylamideab"> | ||
+ | <object type="application/pdf" data="https://static.igem.org/mediawiki/2018/1/19/T--TecCEM--Experiments-Reactants4.pdf" | ||
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+ | </object> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="container mt-3"> | ||
+ | <h1>Nucleic acids</h1> | ||
+ | <h2>Cell transformation</h2> | ||
+ | <div class="row"> | ||
+ | <div class="col"> | ||
+ | <a class="btn-link text-notebook" data-toggle="collapse" href="#creationlysisbbuffer" role="button" | ||
+ | aria-expanded="false" aria-controls="creationlysisbbuffer"> | ||
+ | <h3>Protocol for the creation of lysis buffer P</h3> | ||
+ | </a> | ||
+ | <div class="collapse" id="creationlysisbbuffer"> | ||
+ | <object type="application/pdf" data="https://static.igem.org/mediawiki/2018/e/e9/T--TecCEM--Experiments-Reactants1.pdf" | ||
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+ | </object> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="row"> | ||
+ | <div class="col"> | ||
+ | <a class="btn-link text-notebook" data-toggle="collapse" href="#creationpolyacrylamidegel" role="button" | ||
+ | aria-expanded="false" aria-controls="creationpolyacrylamidegel"> | ||
+ | <h3>Protocol for the creation of polyacrylamide gel</h3> | ||
+ | </a> | ||
+ | <div class="collapse" id="creationpolyacrylamidegel"> | ||
+ | <object type="application/pdf" data="https://static.igem.org/mediawiki/2018/c/c1/T--TecCEM--Experiments-Reactants2.pdf" | ||
+ | width="100%" height="900px"> | ||
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+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="row"> | ||
+ | <div class="col"> | ||
+ | <a class="btn-link text-notebook" data-toggle="collapse" href="#creationthemlysisbuffer" role="button" | ||
+ | aria-expanded="false" aria-controls="creationthemlysisbuffer"> | ||
+ | <h3>Protocol for the creation of thermal lysis buffer</h3> | ||
+ | </a> | ||
+ | <div class="collapse" id="creationthemlysisbuffer"> | ||
+ | <object type="application/pdf" data="https://static.igem.org/mediawiki/2018/2/21/T--TecCEM--Experiments-Reactants3.pdf" | ||
+ | width=" | ||
+ | 100%" height="900px"> | ||
+ | </object> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <h2>Enzyme restriction</h2> | ||
+ | <div class="row"> | ||
+ | <div class="col"> | ||
+ | <a class="btn-link text-notebook" data-toggle="collapse" href="#creationpolyacrylamideab" role="button" | ||
+ | aria-expanded="false" aria-controls="creationpolyacrylamideab"> | ||
+ | <h3>Protocol to make polyacrylamide AB</h3> | ||
+ | </a> | ||
+ | <div class="collapse" id="creationpolyacrylamideab"> | ||
+ | <object type="application/pdf" data="https://static.igem.org/mediawiki/2018/1/19/T--TecCEM--Experiments-Reactants4.pdf" | ||
+ | width="100%" height="900px"> | ||
+ | </object> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="row"> | ||
+ | <div class="col"> | ||
+ | <a class="btn-link text-notebook" data-toggle="collapse" href="#creationpolyacrylamideab" role="button" | ||
+ | aria-expanded="false" aria-controls="creationpolyacrylamideab"> | ||
+ | <h3>Protocol to make polyacrylamide AB</h3> | ||
+ | </a> | ||
+ | <div class="collapse" id="creationpolyacrylamideab"> | ||
+ | <object type="application/pdf" data="https://static.igem.org/mediawiki/2018/1/19/T--TecCEM--Experiments-Reactants4.pdf" | ||
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+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="row"> | ||
+ | <div class="col"> | ||
+ | <a class="btn-link text-notebook" data-toggle="collapse" href="#creationpolyacrylamideab" role="button" | ||
+ | aria-expanded="false" aria-controls="creationpolyacrylamideab"> | ||
+ | <h3>Protocol to make polyacrylamide AB</h3> | ||
+ | </a> | ||
+ | <div class="collapse" id="creationpolyacrylamideab"> | ||
+ | <object type="application/pdf" data="https://static.igem.org/mediawiki/2018/1/19/T--TecCEM--Experiments-Reactants4.pdf" | ||
+ | width="100%" height="900px"> | ||
+ | </object> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <h2>PCR</h2> | ||
+ | <div class="row"> | ||
+ | <div class="col"> | ||
+ | <a class="btn-link text-notebook" data-toggle="collapse" href="#creationlysisbbuffer" role="button" | ||
+ | aria-expanded="false" aria-controls="creationlysisbbuffer"> | ||
+ | <h3>Protocol for the creation of lysis buffer P</h3> | ||
+ | </a> | ||
+ | <div class="collapse" id="creationlysisbbuffer"> | ||
+ | <object type="application/pdf" data="https://static.igem.org/mediawiki/2018/e/e9/T--TecCEM--Experiments-Reactants1.pdf" | ||
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+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="row"> | ||
+ | <div class="col"> | ||
+ | <a class="btn-link text-notebook" data-toggle="collapse" href="#creationpolyacrylamidegel" role="button" | ||
+ | aria-expanded="false" aria-controls="creationpolyacrylamidegel"> | ||
+ | <h3>Protocol for the creation of polyacrylamide gel</h3> | ||
+ | </a> | ||
+ | <div class="collapse" id="creationpolyacrylamidegel"> | ||
+ | <object type="application/pdf" data="https://static.igem.org/mediawiki/2018/c/c1/T--TecCEM--Experiments-Reactants2.pdf" | ||
+ | width="100%" height="900px"> | ||
+ | </object> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <h2>Plasmid extraction</h2> | ||
+ | <div class="row"> | ||
+ | <div class="col"> | ||
+ | <a class="btn-link text-notebook" data-toggle="collapse" href="#creationpolyacrylamideab" role="button" | ||
+ | aria-expanded="false" aria-controls="creationpolyacrylamideab"> | ||
+ | <h3>Protocol to make polyacrylamide AB</h3> | ||
+ | </a> | ||
+ | <div class="collapse" id="creationpolyacrylamideab"> | ||
+ | <object type="application/pdf" data="https://static.igem.org/mediawiki/2018/1/19/T--TecCEM--Experiments-Reactants4.pdf" | ||
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+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="row"> | ||
+ | <div class="col"> | ||
+ | <a class="btn-link text-notebook" data-toggle="collapse" href="#creationpolyacrylamideab" role="button" | ||
+ | aria-expanded="false" aria-controls="creationpolyacrylamideab"> | ||
+ | <h3>Protocol to make polyacrylamide AB</h3> | ||
+ | </a> | ||
+ | <div class="collapse" id="creationpolyacrylamideab"> | ||
+ | <object type="application/pdf" data="https://static.igem.org/mediawiki/2018/1/19/T--TecCEM--Experiments-Reactants4.pdf" | ||
+ | width="100%" height="900px"> | ||
</object> | </object> | ||
</div> | </div> |
Revision as of 22:18, 17 October 2018
Experiments
This is our experiment section. Here we compile important protocols for the development of TecTissue, ranging from our bacterial transformation procedures to our cell proliferation assays. We also address cell culture maintenance and protein loaded chitosan nanoparticles. Here you may find the protocol for our growth factor delivery to damaged cells and how much harm can be inflicted in vitro.