Difference between revisions of "Team:Gifu/Results"
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<p>mRFP shows the clear fluorescence. mRFP tem. and mRFP PCR shows the same result. mRFP shown in the figure is mRFP tem.</legend></center> | <p>mRFP shows the clear fluorescence. mRFP tem. and mRFP PCR shows the same result. mRFP shown in the figure is mRFP tem.</legend></center> | ||
<img src="https://static.igem.org/mediawiki/2018/f/f7/T--Gifu--UV_myTXTL.jpg" width="700px"><br> | <img src="https://static.igem.org/mediawiki/2018/f/f7/T--Gifu--UV_myTXTL.jpg" width="700px"><br> | ||
| − | <center> <legend><b>Fig.4 Expression in mtTXTL under UV</b><p>P: Positive ( | + | <center> <legend><b>Fig.4 Expression in mtTXTL under UV</b><p>P: Positive (eGFP), N: Negative (Water)</p> |
| − | <p>eGFP turned into green.</legend></center> | + | <p>Positive (eGFP) turned into green.</legend></center> |
</div> | </div> | ||
Revision as of 22:28, 17 October 2018
Results
PCR
The result of PCR is shown in Fig.1. All PCR was performed by primers in table 1. All PCR were succeeded. We used PrimeSTAR Max premix for amplification.
Cell-free expression
Here we show the result of expression. (Fig. 4~7) Seq.2 (monomer RFP, mRFP) was expressed well in PURE system and slightly expressed in myTXTL. Seq.1 (polymer RFP, pRFP) has no fluorescence.
