Difference between revisions of "Team:Gifu/Results"
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<p>Positive (eGFP) shows green color.</p></legend></center> | <p>Positive (eGFP) shows green color.</p></legend></center> | ||
<img src="https://static.igem.org/mediawiki/2018/7/7d/T--Gifu--UV_PURE.jpg" width="700px"><br> | <img src="https://static.igem.org/mediawiki/2018/7/7d/T--Gifu--UV_PURE.jpg" width="700px"><br> | ||
| − | <center> <legend><b>Fig.4 Expression in PURE system under UV | + | <center> <legend><b>Fig.4 Expression in PURE system under UV</b><p>P: Positive (DHFR gene), N: Negative (Water), tem.: Template DNA</p> |
<p>mRFP shows the clear fluorescence. mRFP tem. and mRFP PCR shows the same result. mRFP shown in the figure is mRFP tem.</legend></center> | <p>mRFP shows the clear fluorescence. mRFP tem. and mRFP PCR shows the same result. mRFP shown in the figure is mRFP tem.</legend></center> | ||
<img src="https://static.igem.org/mediawiki/2018/f/f7/T--Gifu--UV_myTXTL.jpg" width="700px"><br> | <img src="https://static.igem.org/mediawiki/2018/f/f7/T--Gifu--UV_myTXTL.jpg" width="700px"><br> | ||
Revision as of 22:29, 17 October 2018
Results
PCR
The result of PCR is shown in Fig.1. All PCR was performed by primers in table 1. All PCR were succeeded. We used PrimeSTAR Max premix for amplification.
Cell-free expression
Here we show the result of expression. (Fig. 4~7) Seq.2 (monomer RFP, mRFP) was expressed well in PURE system and slightly expressed in myTXTL. Seq.1 (polymer RFP, pRFP) has no fluorescence.
