Difference between revisions of "Team:Munich/cfp gRNA.html"

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<a href="HERE" target="_blank">Cpf1</a>,  
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<a href="HERE" target="_blank">Cpf1 digest</a>,  
 
<a href="HERE" target="_blank">Agarose gel</a>
 
<a href="HERE" target="_blank">Agarose gel</a>
 
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Revision as of 22:44, 17 October 2018

Generation of gRNA (the DNA for the transcription)

2018/05/25
Participants: Keno Eilers
Protocol: gRNA production
Notes: Work at RNAse free bench.
Results: Coding and non-coding part of digestion site 1, 2 and 3 are annealed.

Generating MS2 RNA and gRNA

2018/05/28
Participants: Keno Eilers
Protocol: gRNA production, Phenol Chloroform precipitation
Notes: No notes.
Results: DNA has been used for MS2production and gRNA for cpf digest.

Cpf1 digest -gRNA production

2018/05/29
Participants: Keno Eilers
Protocol: Cpf1 digest, Urea gel
Notes: No notes.
Results: No gRNA visible.

Cpf1 digest

2018/06/06
Participants: Keno Eilers
Protocol: Cpf1 digest, Agarose gel
Notes: No notes.
Results: No bands visible.

Generating new gRNA

2018/06/14
Participants: Keno Eilers
Protocol: gRNA production, Phenol Chloroform precipitation
Notes: Used DNAse I
Results: DNA has been used for Urea Page gel

gRNA generation and Urea Page Gel

2018/06/18
Participants: Keno Eilers
Protocol: gRNA production, Urea gel
Notes: No notes.
Results: gRNA1 620 ng/µl
gRNA2 591 ng/µl

Cpf1 digest with gRNA

2018/06/26
Participants: Keno Eilers
Protocol: Cpf1 digest, Agarose gel
Notes: No notes.
Results: No bands, T7 DNA not clean enough.