Difference between revisions of "Team:Gifu/Results"
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<center> <legend><b>Fig.4 Expression in PURE system under UV</b><p>P: Positive (DHFR gene), N: Negative (Water), tem.: Template DNA</p> | <center> <legend><b>Fig.4 Expression in PURE system under UV</b><p>P: Positive (DHFR gene), N: Negative (Water), tem.: Template DNA</p> | ||
<p>mRFP shows the clear fluorescence. mRFP tem. and mRFP PCR shows the same result. mRFP shown in the figure is mRFP tem.</legend></center> | <p>mRFP shows the clear fluorescence. mRFP tem. and mRFP PCR shows the same result. mRFP shown in the figure is mRFP tem.</legend></center> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/f/f7/T--Gifu--UV_myTXTL.jpg" width="700px"><br> | ||
| + | <center> <legend><b>Fig.4 Expression in mtTXTL under UV</b><p>P: Positive (eGFP), N: Negative (Water)</p> | ||
| + | <p>Positive (eGFP) turned into green.</legend></center> | ||
| + | <h1>SDSPAGE</h1> | ||
| + | <p>We performed SDSPAGE to confirm whether pRFP was expressed or not. The result is shown below.</p> | ||
<img src="https://static.igem.org/mediawiki/2018/f/f7/T--Gifu--UV_myTXTL.jpg" width="700px"><br> | <img src="https://static.igem.org/mediawiki/2018/f/f7/T--Gifu--UV_myTXTL.jpg" width="700px"><br> | ||
<center> <legend><b>Fig.4 Expression in mtTXTL under UV</b><p>P: Positive (eGFP), N: Negative (Water)</p> | <center> <legend><b>Fig.4 Expression in mtTXTL under UV</b><p>P: Positive (eGFP), N: Negative (Water)</p> | ||
Revision as of 22:56, 17 October 2018
Results
PCR
The result of PCR is shown in Fig.1. All PCR was performed by primers in table 1. All PCR were succeeded. We used PrimeSTAR Max premix for amplification.
Cell-free expression
Here we show the result of expression. (Fig. 4~7) Seq.2 (monomer RFP, mRFP) was expressed well in PURE system and slightly expressed in myTXTL. Seq.1 (polymer RFP, pRFP) has no fluorescence.
We performed SDSPAGE to confirm whether pRFP was expressed or not. The result is shown below.
SDSPAGE