Difference between revisions of "Team:Gifu/Results"
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<p>We performed SDSPAGE to confirm whether pRFP was expressed or not. The result is shown below.</p> | <p>We performed SDSPAGE to confirm whether pRFP was expressed or not. The result is shown below.</p> | ||
<img src="https://static.igem.org/mediawiki/2018/7/76/T--Gifu--SDSPAGE.jpg" width="700px"><br> | <img src="https://static.igem.org/mediawiki/2018/7/76/T--Gifu--SDSPAGE.jpg" width="700px"><br> | ||
| − | <img src="https://static.igem.org/mediawiki/2018/ | + | <img src="https://static.igem.org/mediawiki/2018/6/6f/T--Gifu--SDSPAGE-LANE.png" width="700px"><br> |
<center> <legend><b>Fig.4 SDSPAGE</b> | <center> <legend><b>Fig.4 SDSPAGE</b> | ||
</div> | </div> | ||
Revision as of 23:01, 17 October 2018
Results
PCR
The result of PCR is shown in Fig.1. All PCR was performed by primers in table 1. All PCR were succeeded. We used PrimeSTAR Max premix for amplification.
Cell-free expression
Here we show the result of expression. (Fig. 4~7) Seq.2 (monomer RFP, mRFP) was expressed well in PURE system and slightly expressed in myTXTL. Seq.1 (polymer RFP, pRFP) has no fluorescence.
We performed SDSPAGE to confirm whether pRFP was expressed or not. The result is shown below.
SDSPAGE
