Difference between revisions of "Team:Gifu/Results"
| Line 44: | Line 44: | ||
<center> <legend><b>Fig.4 Expression in mtTXTL under UV</b><p>P: Positive (eGFP), N: Negative (Water)</p> | <center> <legend><b>Fig.4 Expression in mtTXTL under UV</b><p>P: Positive (eGFP), N: Negative (Water)</p> | ||
<p>Positive (eGFP) turned into green.</legend></center> | <p>Positive (eGFP) turned into green.</legend></center> | ||
| + | |||
<h1>SDSPAGE</h1> | <h1>SDSPAGE</h1> | ||
<p>We performed SDSPAGE to confirm whether pRFP was expressed or not. The result is shown below.</p> | <p>We performed SDSPAGE to confirm whether pRFP was expressed or not. The result is shown below.</p> | ||
Revision as of 23:02, 17 October 2018
Results
PCR
The result of PCR is shown in Fig.1. All PCR was performed by primers in table 1. All PCR were succeeded. We used PrimeSTAR Max premix for amplification.
Cell-free expression
Here we show the result of expression. (Fig. 4~7) Seq.2 (monomer RFP, mRFP) was expressed well in PURE system and slightly expressed in myTXTL. Seq.1 (polymer RFP, pRFP) has no fluorescence.
We performed SDSPAGE to confirm whether pRFP was expressed or not. The result is shown below.
SDSPAGE
