Difference between revisions of "Team:Warwick/Design"

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<link rel="stylesheet" href="https://2018.igem.org/Team:Warwick/CSS/GenericPageCSS?action=raw&ctype=text/css">
 
<link rel="stylesheet" href="https://2018.igem.org/Team:Warwick/CSS/GenericPageCSS?action=raw&ctype=text/css">
 
<script src="https://ajax.googleapis.com/ajax/libs/jquery/3.3.1/jquery.min.js"></script>
 
<script src="https://ajax.googleapis.com/ajax/libs/jquery/3.3.1/jquery.min.js"></script>
<style>.Contents p{font-size: 175% !important} .Contents:nth-child(even) h2{color: white !important;} .Contents h2{font-size: 2vw;}</style>
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<style>.Contents p{font-size: 175% !important} .Contents:nth-child(even) h2{color: white !important;} .Contents h2{line-height: normal; font-size: 2vw;}</style>
  
 
<script>
 
<script>
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         $("#inorgIMG").css("background-color", "white");
 
         $("#inorgIMG").css("background-color", "white");
  
         $("#Content1").html("<h2>Synthetic Gene Pathway</h2><img style='width: 100%; background-color: white !important; border-radius: 50px;' src='https://static.igem.org/mediawiki/2018/3/31/T--Warwick--ribodesign1.png'><br><p>ATC induces the circuit.<br><br>TetR is disabled by ATC.<br><br>The disabled TetR can no longer inhibit the transcription of the pathogen imitating <a href='http://parts.igem.org/wiki/index.php/Part:BBa_K2841531'>sRNA </a>.<br><br>The <a href='http://parts.igem.org/wiki/index.php/Part:BBa_K2841531'>sRNA</a> can bind to the constitutively expressed riboregulated <a href='http://parts.igem.org/Part:BBa_K2841541'>gRNA</a>, exposing the targeting domain.<br><br>The exposed <a href='http://parts.igem.org/wiki/index.php/Part:BBa_K2841510'>dCAS9</a> recognising cis-acting factor within the <a href='http://parts.igem.org/Part:BBa_K2841541'>gRNA</a> binds to the constitutively expressed <a href='http://parts.igem.org/wiki/index.php/Part:BBa_K2841510'>dCAS9</a> protein. <br><br>Once enabled the dCAS9 protein will bind to the <a href='http://parts.igem.org/wiki/index.php/Part:BBa_K2841517'>LacIq</a> promoter, inhibiting the expression of the LacI repressor protein.<br><br>Once the <a href='http://parts.igem.org/wiki/index.php/Part:BBa_K2841517'>LacI</a> repressor is inhibited, every pLAC will be upregulated due to lack of inhibition<br><br>The sfGFP will be synthesised from the pLAC, resulting in a green fluorescence.<br><br><br>The sRNA and dCAS9 are found on a Kanamycin resistant plasmid.<br><br><br>The gRNA and GFP are found on a Chloramphenicol plasmid.<br><br>The LacI is found on the Z1 cassette found in MG1655 Z1 cells. The Z1 cassette contains a set of repressors for the cell to utilise.<br><br></p>");
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         $("#Content1").html("<h2>Synthetic Gene Pathway</h2><img style='width: 100%; background-color: white !important; border-radius: 50px;' src='https://static.igem.org/mediawiki/2018/3/31/T--Warwick--ribodesign1.png'><br><br><p>ATC induces the circuit.<br><br>TetR is disabled by ATC.<br><br>The disabled TetR can no longer inhibit the transcription of the pathogen imitating <a href='http://parts.igem.org/wiki/index.php/Part:BBa_K2841531'>sRNA </a>.<br><br>The <a href='http://parts.igem.org/wiki/index.php/Part:BBa_K2841531'>sRNA</a> can bind to the constitutively expressed riboregulated <a href='http://parts.igem.org/Part:BBa_K2841541'>gRNA</a>, exposing the targeting domain.<br><br>The exposed <a href='http://parts.igem.org/wiki/index.php/Part:BBa_K2841510'>dCAS9</a> recognising cis-acting factor within the <a href='http://parts.igem.org/Part:BBa_K2841541'>gRNA</a> binds to the constitutively expressed <a href='http://parts.igem.org/wiki/index.php/Part:BBa_K2841510'>dCAS9</a> protein. <br><br>Once enabled the dCAS9 protein will bind to the <a href='http://parts.igem.org/wiki/index.php/Part:BBa_K2841517'>LacIq</a> promoter, inhibiting the expression of the LacI repressor protein.<br><br>Once the <a href='http://parts.igem.org/wiki/index.php/Part:BBa_K2841517'>LacI</a> repressor is inhibited, every pLAC will be upregulated due to lack of inhibition<br><br>The sfGFP will be synthesised from the pLAC, resulting in a green fluorescence.<br><br><br>The sRNA and dCAS9 are found on a Kanamycin resistant plasmid.<br><br><br>The gRNA and GFP are found on a Chloramphenicol plasmid.<br><br>The LacI is found on the Z1 cassette found in MG1655 Z1 cells. The Z1 cassette contains a set of repressors for the cell to utilise.<br><br></p>");
  
 
         $("#Content2").html("<h2>Plasmids and Sequences</h2><img src='https://static.igem.org/mediawiki/2018/5/5d/T--Warwick--ribodesign2.png'><img style='width: 100%' src='https://static.igem.org/mediawiki/2018/5/59/T--Warwick--ribodesign3.png'>");
 
         $("#Content2").html("<h2>Plasmids and Sequences</h2><img src='https://static.igem.org/mediawiki/2018/5/5d/T--Warwick--ribodesign2.png'><img style='width: 100%' src='https://static.igem.org/mediawiki/2018/5/59/T--Warwick--ribodesign3.png'>");

Revision as of 23:02, 17 October 2018

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Design