Difference between revisions of "Team:HUST-China/Composite part"

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                               <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Modeling overview">Modeling overview</a></li>
 
                               <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Modeling overview">Modeling overview</a></li>
                               <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Comparison between PSB">Comparison between PSB</a></li>
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                               <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/model_of_systems">Model of systems</a></li>
 
                               <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Sort of three genes">Sort of three genes</a></li>
 
                               <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Sort of three genes">Sort of three genes</a></li>
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                               <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Software">Software</a></li>  
 
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                               <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Human Practices">Human Practices</a></li>
                               <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Education&Engagement">Education&Engagement</a></li>  
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                               <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Education_Engagement">Education&Engagement</a></li>  
 
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                               <li><a class="waves-effect waves-dark" href="https://2018.igem.org/Team:HUST-China/Attributions">Attributions</a></li>
 
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Revision as of 23:08, 17 October 2018

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Composite part

1.1 Synechocystis sp. PCC 6803(cyanobacteria)

Name Description
BBa_K2533000 RBS-ldhD-RBS-lldP
BBa_K2533001 RBS-ldhDC-RBS-lldP
BBa_K2533002 RBS-ldhDnARSdR-lldP
BBa_K2533003 RBS-ldhDARSdR-lldP
BBa_K2533014 RBS-TH-RBS-gldA-RBS-LLDP
BBa_K2533016 RBS-ldhD-FLAG-RBS-lldP-his tag
BBa_K2533017 RBS-ldhDC-FLAG-RBS-lldP-his tag
BBa_K2533018 RBS-ldhDnARSdR-FLAG-RBS-lldP-his tag
BBa_K2533019 RBS-ldhDARSdR-FLAG-RBS-lldP-his tag

BBa_K2533000, BBa_K2533001, BBa_K2533002, BBa_K2533003, BBa_K2533014

BBa_K2533016, BBa_K2533017, BBa_K2533018, BBa_K2533019

1.2. Rhodopseudomonas palustris

Name Description
BBa_K2533045 RBS-mleS
BBa_K2533046 RBS-lldP
BBa_K2533047 RBS-ldhA
BBa_K2533048 RBS-mleS-RBS-lldP
BBa_K2533049 RBS-lldP-RBS-ldhA
BBa_K2533050 mleS-lldp-ldhA
BBa_K2533051 mleS-lldP-TT
BBa_K2533052 RBS-lldP-RBS-ldhA-TT
BBa_K2533053 RBS-mleS-RBS-lldP-RBS-ldhA-TT
BBa_K2533054 RBS-ldhA-RBS-lldP
BBa_K2533055 RBS-ldhA-RBS-lldP-TT

BBa_K2533050, BBa_K2533048, BBa_K2533054, BBa_K2533049

Our photosynthetic microorganism system is consist of Synechocystis sp. PCC 6803(cyanobacteria) and Rhodopseudomonas palustris. They are all modified to produce lactate to improve the electrogenic efficiency of Shewanella(S.oneidensis).

2.1. Shewanella(S.oneidensis)

Name Description
BBa_K2533030 RBS-dld
BBa_K2533031 RBS-lldEFG
BBa_K2533032 RBS-dld-TT-Ptac-RBS-lldEFG
BBa_K2533033 RBS-gapA
BBa_K2533034 RBS-mdh
BBa_K2533035 RBS-gapA-RBS-mdh-TT
BBa_K2533036 RBS-pflB
BBa_K2533037 RBS-fdh
BBa_K2533038 RBS-pflB-RBS-fdh
BBa_K2533039 RBS-gapA-RBS-mdh-TT-Ptac-RBS-pflB-RBS-fdh
BBa_K2533056 PlacIq-lacI-Ptac-RBS-gapA

BBa_K2533030, BBa_K2533031, BBa_K2533032, BBa_K2533033, BBa_K2533034, BBa_K2533036, BBa_K2533037

BBa_K2533035, BBa_K2533038, BBa_K2533039

Our MFC produces electricity by Shewanella(S.oneidensis) through transfer chemical energy into electric energy. As a result, we design these parts to strengthen the ability of Shewanella(S.oneidensis) to absorb lactate,with an intention to boost the efficiency of electrogenic microorganism system.