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$("#Content1").html("<h2>Synthetic Gene Pathway</h2><img style='width: 100%; background-color: white !important; border-radius: 50px;' src='https://static.igem.org/mediawiki/2018/3/31/T--Warwick--ribodesign1.png'><br><br><p>ATC induces the circuit.<br><br>TetR is disabled by ATC.<br><br>The disabled TetR can no longer inhibit the transcription of the pathogen imitating <a href='http://parts.igem.org/wiki/index.php/Part:BBa_K2841531'>sRNA </a>.<br><br>The <a href='http://parts.igem.org/wiki/index.php/Part:BBa_K2841531'>sRNA</a> can bind to the constitutively expressed riboregulated <a href='http://parts.igem.org/Part:BBa_K2841541'>gRNA</a>, exposing the targeting domain.<br><br>The exposed <a href='http://parts.igem.org/wiki/index.php/Part:BBa_K2841510'>dCAS9</a> recognising cis-acting factor within the <a href='http://parts.igem.org/Part:BBa_K2841541'>gRNA</a> binds to the constitutively expressed <a href='http://parts.igem.org/wiki/index.php/Part:BBa_K2841510'>dCAS9</a> protein. <br><br>Once enabled the dCAS9 protein will bind to the <a href='http://parts.igem.org/wiki/index.php/Part:BBa_K2841517'>LacIq</a> promoter, inhibiting the expression of the LacI repressor protein.<br><br>Once the <a href='http://parts.igem.org/wiki/index.php/Part:BBa_K2841517'>LacI</a> repressor is inhibited, every pLAC will be upregulated due to lack of inhibition<br><br>The sfGFP will be synthesised from the pLAC, resulting in a green fluorescence.<br><br><br>The sRNA and dCAS9 are found on a Kanamycin resistant plasmid.<br><br><br>The gRNA and GFP are found on a Chloramphenicol plasmid.<br><br>The LacI is found on the Z1 cassette found in MG1655 Z1 cells. The Z1 cassette contains a set of repressors for the cell to utilise.<br><br></p>"); | $("#Content1").html("<h2>Synthetic Gene Pathway</h2><img style='width: 100%; background-color: white !important; border-radius: 50px;' src='https://static.igem.org/mediawiki/2018/3/31/T--Warwick--ribodesign1.png'><br><br><p>ATC induces the circuit.<br><br>TetR is disabled by ATC.<br><br>The disabled TetR can no longer inhibit the transcription of the pathogen imitating <a href='http://parts.igem.org/wiki/index.php/Part:BBa_K2841531'>sRNA </a>.<br><br>The <a href='http://parts.igem.org/wiki/index.php/Part:BBa_K2841531'>sRNA</a> can bind to the constitutively expressed riboregulated <a href='http://parts.igem.org/Part:BBa_K2841541'>gRNA</a>, exposing the targeting domain.<br><br>The exposed <a href='http://parts.igem.org/wiki/index.php/Part:BBa_K2841510'>dCAS9</a> recognising cis-acting factor within the <a href='http://parts.igem.org/Part:BBa_K2841541'>gRNA</a> binds to the constitutively expressed <a href='http://parts.igem.org/wiki/index.php/Part:BBa_K2841510'>dCAS9</a> protein. <br><br>Once enabled the dCAS9 protein will bind to the <a href='http://parts.igem.org/wiki/index.php/Part:BBa_K2841517'>LacIq</a> promoter, inhibiting the expression of the LacI repressor protein.<br><br>Once the <a href='http://parts.igem.org/wiki/index.php/Part:BBa_K2841517'>LacI</a> repressor is inhibited, every pLAC will be upregulated due to lack of inhibition<br><br>The sfGFP will be synthesised from the pLAC, resulting in a green fluorescence.<br><br><br>The sRNA and dCAS9 are found on a Kanamycin resistant plasmid.<br><br><br>The gRNA and GFP are found on a Chloramphenicol plasmid.<br><br>The LacI is found on the Z1 cassette found in MG1655 Z1 cells. The Z1 cassette contains a set of repressors for the cell to utilise.<br><br></p>"); | ||
− | $("#Content2").html("<h2>Plasmids and Sequences</h2><img src='https://static.igem.org/mediawiki/2018/5/5d/T--Warwick--ribodesign2.png'><img style='width: 100%' src='https://static.igem.org/mediawiki/2018/5/59/T--Warwick--ribodesign3.png'>"); | + | $("#Content2").html("<h2>Plasmids and Sequences</h2><img src='https://static.igem.org/mediawiki/2018/5/5d/T--Warwick--ribodesign2.png'><img style='width: 100%' src='https://static.igem.org/mediawiki/2018/5/59/T--Warwick--ribodesign3.png'><img style='width: 100%' src='https://static.igem.org/mediawiki/2018/f/f5/T--Warwick--ribodesign4.png'><img style='width: 100%' src='https://static.igem.org/mediawiki/2018/d/d2/T--Warwick--ribodesign5.png'><img style='width: 100%' src='https://static.igem.org/mediawiki/2018/d/d2/T--Warwick--ribodesign5.png'>"); |
} | } | ||
Revision as of 23:14, 17 October 2018
igem@warwick.ac.uk