Difference between revisions of "Team:Warwick/Design"

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         $("#Content2").html("<h2>Plasmids and Sequences</h2><img src='https://static.igem.org/mediawiki/2018/5/5d/T--Warwick--ribodesign2.png'><img style='width: 80%' src='https://static.igem.org/mediawiki/2018/5/59/T--Warwick--ribodesign3.png'><img style='width: 80%' src='https://static.igem.org/mediawiki/2018/f/f5/T--Warwick--ribodesign4.png'><img style='width: 80%' src='https://static.igem.org/mediawiki/2018/d/d2/T--Warwick--ribodesign5.png'><p>Every plasmid in genbank format: <a href='http://parts.igem.org/File:T--Warwick--2018-Entire-Project.zip'>here</a><br><br>We have the full plasmid<br><br>gRNA secondary structure prediction<br><br>ATGTTTAAACACGGATGTTTTATACTAATAGTCTTTTTTTTAGCCAGCTGCTTGTCAGGCTGTCTGGGGGTTTTATGGACTGGGGCAACTCTAGCCTATGACCGGCACAATGTATATAAAAAATTAGATGATTATAATTTAATAAAAGCTCTTAATGATGTTTTAGCTGTCAACCGGACATTTAAAAACTCAGAGACAGTACTGGATATTGCTGTGTTCAACAGAGATATTTTAATAGCAGGGCATGTTCCAACCCAGGAGTTGTACGATGAATTACAACTACGTCTAAGCAAAGTTAAAGGATACAGGCGGTTATTCAATCGTGTAATTATCAATAAAATGCCATCCAACTCGATCCAGGACAGCTGGATTACCACGAAAATCCGTAGCCAAATTTTTGCAGACTCTTCAATCGATCCTAACGCTTTCAAGGTGGTTACTTCTGATCGTGTTGTCTATTTGATGGGAGATGTTCAGACAGAACAAGCTGAAAAAGTGATTAAAATTGCAAGATACACAGGTGGTGTACAGAAAGTAATAAAACTTATGCGGTATTATACTTATCAAACCAGTACGAATATGGCCTGA<br><br>NuPack - accurate prediction (why 1999 paper)<br><br>2d molecular prediction (all constructs)</p>");
 
         $("#Content2").html("<h2>Plasmids and Sequences</h2><img src='https://static.igem.org/mediawiki/2018/5/5d/T--Warwick--ribodesign2.png'><img style='width: 80%' src='https://static.igem.org/mediawiki/2018/5/59/T--Warwick--ribodesign3.png'><img style='width: 80%' src='https://static.igem.org/mediawiki/2018/f/f5/T--Warwick--ribodesign4.png'><img style='width: 80%' src='https://static.igem.org/mediawiki/2018/d/d2/T--Warwick--ribodesign5.png'><p>Every plasmid in genbank format: <a href='http://parts.igem.org/File:T--Warwick--2018-Entire-Project.zip'>here</a><br><br>We have the full plasmid<br><br>gRNA secondary structure prediction<br><br>ATGTTTAAACACGGATGTTTTATACTAATAGTCTTTTTTTTAGCCAGCTGCTTGTCAGGCTGTCTGGGGGTTTTATGGACTGGGGCAACTCTAGCCTATGACCGGCACAATGTATATAAAAAATTAGATGATTATAATTTAATAAAAGCTCTTAATGATGTTTTAGCTGTCAACCGGACATTTAAAAACTCAGAGACAGTACTGGATATTGCTGTGTTCAACAGAGATATTTTAATAGCAGGGCATGTTCCAACCCAGGAGTTGTACGATGAATTACAACTACGTCTAAGCAAAGTTAAAGGATACAGGCGGTTATTCAATCGTGTAATTATCAATAAAATGCCATCCAACTCGATCCAGGACAGCTGGATTACCACGAAAATCCGTAGCCAAATTTTTGCAGACTCTTCAATCGATCCTAACGCTTTCAAGGTGGTTACTTCTGATCGTGTTGTCTATTTGATGGGAGATGTTCAGACAGAACAAGCTGAAAAAGTGATTAAAATTGCAAGATACACAGGTGGTGTACAGAAAGTAATAAAACTTATGCGGTATTATACTTATCAAACCAGTACGAATATGGCCTGA<br><br>NuPack - accurate prediction (why 1999 paper)<br><br>2d molecular prediction (all constructs)</p>");
  
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         $("#Content3").html("<img style='width: 80%; background-color: white;' src='https://static.igem.org/mediawiki/2018/7/77/T--Warwick--ribodesign6.png'><br><br><br><img style='width: 80%; background-color: white;' src='https://static.igem.org/mediawiki/2018/3/33/T--Warwick--ribodesign7.png'><br><br><br><img style='width: 80%; background-color: white;' src='https://static.igem.org/mediawiki/2018/9/94/T--Warwick--ribodesign8.png'><br><br><br><img style='width: 80%; background-color: white;' src=''><br><p>We used centrifold method of folding with RNA composer to get some <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2703931/'>results</a></p>");
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         $("#Content3").html("<img style='width: 80%; background-color: white;' src='https://static.igem.org/mediawiki/2018/7/77/T--Warwick--ribodesign6.png'><br><br><br><img style='width: 80%; background-color: white;' src='https://static.igem.org/mediawiki/2018/3/33/T--Warwick--ribodesign7.png'><br><br><br><img style='width: 80%; background-color: white;' src='https://static.igem.org/mediawiki/2018/9/94/T--Warwick--ribodesign8.png'><br><br><br><img style='width: 80%; background-color: white;' src='https://static.igem.org/mediawiki/2018/a/aa/T--Warwick--2018-DNAwhole.gif'><br><p>We used centrifold method of folding with RNA composer to get some <a href='https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2703931/'>results</a></p>");
  
 
         $("#Content4").html("<h2>Alpha RNA Construct:</h2><br><p>CCCAGACAGCCTGAC AAAGGTTTTGCAC GCTAAAAAAAAGACT GTGCAAAACCTTTCGCGGTAGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCG</p><br><br><br><h2>Alpha RNA Pathogen-Imitating Region</h2><br><p>AGTCTTTTTTTTAGC CAGCTGCTT GTCAGGCTGTCTGGGatccttagcgaaagctaaggatttttttt</p><br><br><br><h2>Beta RNA Construct:</h2><br>p>GTACACCACCTGTGTA AAAGGTTTTGCAC TTTTAATCACTTTTT GTGCAAAACCTTTCGCGGTAGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCG</p><br><br><br><h2>Beta RNA Pathogen-Imitating Region</h2><br><p>AAAAAGTGATTAAAA TTGCAAGAT<br>ACACAGGTGGTGTACatccttagcgaaagctaaggatttttttt</p><br><br><br><h2>Gamma RNA Construct:</h2><br><p>GGTTGACAGCTAAAA AAAGGTTTTGCAC GAGCTTTTATTAAATTATAATC GTGCAAAACCTTTCGCGGTAGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCG<br><br><br><h2>Gamma RNA Pathogen-Imitating Region</h2><br><p>GATTATAATTTAATAAAAGCTC TTAATGATG TTTTAGCTGTCAACCatccttagcgaaagctaaggatttttttt</p><br><br><br><h2>Delta RNA Construct:</h2><br><p>TAGGCTAGAGTTGCCAAAGGTTTTGCAC  AAAACCCCCAGACAG GTGCAAAACCTTTCGCGGTAGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCG</p><br><br><br><h2>Delta RNA Pathogen-Imitating Region</h2><br><p>CTGTCTGGGGGTTTTATGGACTGGGGCAACTCTAGCCTAatccttagcgaaagctaaggatttttttt</p><br><br><br><h2>Epsilon RNA Construct:</h2><br><p>TCGAGTTGGATGGCAAAAGGTTTTGCAC  TAATTACACGATTGAATAACCGCC GTGCAAAACCTTTCGCGGTAGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCG</p><br><br><br><h2>Epsilon RNA Pathogen-Imitating Region:</h2><br><p>GGCGGTTATTCAATCGTGTAATTATCAATAAAATGCCATCCAACTCGAatccttagcgaaagctaaggatttttttt</p>");
 
         $("#Content4").html("<h2>Alpha RNA Construct:</h2><br><p>CCCAGACAGCCTGAC AAAGGTTTTGCAC GCTAAAAAAAAGACT GTGCAAAACCTTTCGCGGTAGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCG</p><br><br><br><h2>Alpha RNA Pathogen-Imitating Region</h2><br><p>AGTCTTTTTTTTAGC CAGCTGCTT GTCAGGCTGTCTGGGatccttagcgaaagctaaggatttttttt</p><br><br><br><h2>Beta RNA Construct:</h2><br>p>GTACACCACCTGTGTA AAAGGTTTTGCAC TTTTAATCACTTTTT GTGCAAAACCTTTCGCGGTAGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCG</p><br><br><br><h2>Beta RNA Pathogen-Imitating Region</h2><br><p>AAAAAGTGATTAAAA TTGCAAGAT<br>ACACAGGTGGTGTACatccttagcgaaagctaaggatttttttt</p><br><br><br><h2>Gamma RNA Construct:</h2><br><p>GGTTGACAGCTAAAA AAAGGTTTTGCAC GAGCTTTTATTAAATTATAATC GTGCAAAACCTTTCGCGGTAGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCG<br><br><br><h2>Gamma RNA Pathogen-Imitating Region</h2><br><p>GATTATAATTTAATAAAAGCTC TTAATGATG TTTTAGCTGTCAACCatccttagcgaaagctaaggatttttttt</p><br><br><br><h2>Delta RNA Construct:</h2><br><p>TAGGCTAGAGTTGCCAAAGGTTTTGCAC  AAAACCCCCAGACAG GTGCAAAACCTTTCGCGGTAGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCG</p><br><br><br><h2>Delta RNA Pathogen-Imitating Region</h2><br><p>CTGTCTGGGGGTTTTATGGACTGGGGCAACTCTAGCCTAatccttagcgaaagctaaggatttttttt</p><br><br><br><h2>Epsilon RNA Construct:</h2><br><p>TCGAGTTGGATGGCAAAAGGTTTTGCAC  TAATTACACGATTGAATAACCGCC GTGCAAAACCTTTCGCGGTAGTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCG</p><br><br><br><h2>Epsilon RNA Pathogen-Imitating Region:</h2><br><p>GGCGGTTATTCAATCGTGTAATTATCAATAAAATGCCATCCAACTCGAatccttagcgaaagctaaggatttttttt</p>");

Revision as of 23:45, 17 October 2018

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