Difference between revisions of "Team:Rotterdam HR/Results"

Line 42: Line 42:
 
<h1 class="title">Results</h1>
 
<h1 class="title">Results</h1>
  
−
<div class="text-container" style="text-align: left !important">
+
<div class="text-container">
−
<p style="text-align: left !important;">
+
<h1>General</h1>
−
What we have achieved in this project is making 4 composite parts:
+
<p>
 +
Our plan was to create multiple composite parts, which included CooA. These composite parts would have different
 +
promoters and output genes, so we can measure carbon monoxide in multiple ways.
 
</p>
 
</p>
−
<ul>
+
<p>
−
<li>BBa_K2604000 includes the PBad promoter and UreB</li>
+
For further steps we isolated the DNA. The DNA had to be pure for digestions. To be sure the DNA was pure,
−
<li>BBa_K2604001 includes the PBad promoter and pyruvate decarboxylase and alcohol dehydrogenase II enzymes.</li>
+
requirements were set.
−
<li>BBa_K2604003 includes the CooM promoter with UreB and </li>
+
To be pure the DNA needed to have a A260/280 value between 1,80-2,00 and a A260/230 value between 2,00 and 2,20.
−
<li>BBa_K2604006 includes the CooF promoter with pyruvate decarboxylase (pdc) with alcohol dehydrogenase II (AdhB).</li>
+
Hereby there would be looked at the curve of the line also. If the line wasn’t smooth, the DNA was most likely not
−
</ul>
+
pure. A pure sample has a curve like shown in figure 1.
−
<p style="text-align: left !important;">
+
−
These composite parts were made by doing multiple experiments, which the results you can find here. For more
+
−
information about our project, please visit our design page.
+
 
</p>
 
</p>
−
<a class="nav-button" href="https://2018.igem.org/Team:Rotterdam_HR/Parts" style="width: 200px; margin: 5px 0;">Parts page</a>
 
−
<a class="nav-button" href="https://2018.igem.org/Team:Rotterdam_HR/Design" style="width: 200px; margin: 5px 0">Design page</a>
 
 
</div>
 
</div>
 
<div id="footer"></div>
 
<div id="footer"></div>

Revision as of 23:59, 17 October 2018

Results

Results

General

Our plan was to create multiple composite parts, which included CooA. These composite parts would have different promoters and output genes, so we can measure carbon monoxide in multiple ways.

For further steps we isolated the DNA. The DNA had to be pure for digestions. To be sure the DNA was pure, requirements were set. To be pure the DNA needed to have a A260/280 value between 1,80-2,00 and a A260/230 value between 2,00 and 2,20. Hereby there would be looked at the curve of the line also. If the line wasn’t smooth, the DNA was most likely not pure. A pure sample has a curve like shown in figure 1.