Difference between revisions of "Team:Rotterdam HR/Results"

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<h1>General</h1>
 
<h1>General</h1>
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Our plan was to create multiple composite parts, which included CooA. These composite parts would have different
 
Our plan was to create multiple composite parts, which included CooA. These composite parts would have different
 
promoters and output genes, so we can measure carbon monoxide in multiple ways.
 
promoters and output genes, so we can measure carbon monoxide in multiple ways.
 
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For further steps we isolated the DNA. The DNA had to be pure for digestions. To be sure the DNA was pure,
 
For further steps we isolated the DNA. The DNA had to be pure for digestions. To be sure the DNA was pure,
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requirements were set.
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requirements were set.<img src="https://static.igem.org/mediawiki/parts/5/5e/T--Rotterdam_HR--foto1Res.jpeg" style="width: 300px; height: 300px; float: right;">
 
To be pure the DNA needed to have a A260/280 value between 1,80-2,00 and a A260/230 value between 2,00 and 2,20.
 
To be pure the DNA needed to have a A260/280 value between 1,80-2,00 and a A260/230 value between 2,00 and 2,20.
 
Hereby there would be looked at the curve of the line also. If the line wasn’t smooth, the DNA was most likely not
 
Hereby there would be looked at the curve of the line also. If the line wasn’t smooth, the DNA was most likely not

Revision as of 00:01, 18 October 2018

Results

Results

General

Our plan was to create multiple composite parts, which included CooA. These composite parts would have different promoters and output genes, so we can measure carbon monoxide in multiple ways.

For further steps we isolated the DNA. The DNA had to be pure for digestions. To be sure the DNA was pure, requirements were set. To be pure the DNA needed to have a A260/280 value between 1,80-2,00 and a A260/230 value between 2,00 and 2,20. Hereby there would be looked at the curve of the line also. If the line wasn’t smooth, the DNA was most likely not pure. A pure sample has a curve like shown in figure 1.