Difference between revisions of "Team:Munich/cfp gRNA.html"
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<td>Protocol:</td> | <td>Protocol:</td> | ||
<td> | <td> | ||
| − | <a href=" | + | <a href="https://static.igem.org/mediawiki/2018/3/37/T--Munich--Protocol_cpf.pdf" target="_blank">gRNA production </a> |
</td> | </td> | ||
</tr> | </tr> | ||
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<td>Protocol:</td> | <td>Protocol:</td> | ||
<td> | <td> | ||
| − | <a href=" | + | <a href="https://static.igem.org/mediawiki/2018/3/37/T--Munich--Protocol_cpf.pdf" target="_blank">gRNA production</a>, |
| − | <a href=" | + | <a href="https://static.igem.org/mediawiki/2018/0/0f/T--Munich--PhenolChloroformPrecipitation.pdf" target="_blank">Phenol Chloroform precipitation </a> |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 76: | Line 76: | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
<td> | <td> | ||
| − | <a href=" | + | <a href="https://static.igem.org/mediawiki/2018/3/37/T--Munich--Protocol_cpf.pdf" target="_blank">Cpf1 digest</a>, |
| − | <a href=" | + | <a href="https://static.igem.org/mediawiki/2018/d/d0/T--Munich--PreparationUreagel.pdf" target="_blank">Urea gel </a> |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 106: | Line 106: | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
<td> | <td> | ||
| − | <a href=" | + | <a href="https://static.igem.org/mediawiki/2018/3/37/T--Munich--Protocol_cpf.pdf" target="_blank">Cpf1 digest</a>, |
| − | <a href=" | + | <a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a> |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 136: | Line 136: | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
<td> | <td> | ||
| − | <a href=" | + | <a href="https://static.igem.org/mediawiki/2018/3/37/T--Munich--Protocol_cpf.pdf" target="_blank">gRNA production</a>, |
| − | <a href=" | + | <a href="https://static.igem.org/mediawiki/2018/0/0f/T--Munich--PhenolChloroformPrecipitation.pdf" target="_blank">Phenol Chloroform precipitation</a> |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 166: | Line 166: | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
<td> | <td> | ||
| − | <a href=" | + | <a href="https://static.igem.org/mediawiki/2018/3/37/T--Munich--Protocol_cpf.pdf" target="_blank">gRNA production</a>, |
| − | <a href=" | + | <a href="https://static.igem.org/mediawiki/2018/d/d0/T--Munich--PreparationUreagel.pdf" target="_blank">Urea gel</a> |
</td> | </td> | ||
</tr> | </tr> | ||
| Line 198: | Line 198: | ||
<td>Protocol:</td> | <td>Protocol:</td> | ||
<td> | <td> | ||
| − | <a href=" | + | <a href="https://static.igem.org/mediawiki/2018/3/37/T--Munich--Protocol_cpf.pdf" target="_blank">Cpf1 digest</a>, |
| − | <a href=" | + | <a href="https://static.igem.org/mediawiki/2018/b/be/T--Munich--WL1_Agarose-Gel_electrophoresis.pdf" target="_blank">Agarose gel</a> |
</td> | </td> | ||
</td> | </td> | ||
Revision as of 00:09, 18 October 2018
Generation of gRNA (the DNA for the transcription)
2018/05/25| Participants: | Keno Eilers |
| Protocol: | gRNA production | Notes: | Work at RNAse free bench. | Results: | Coding and non-coding part of digestion site 1, 2 and 3 are annealed. |
Generating MS2 RNA and gRNA
2018/05/28| Participants: | Keno Eilers |
| Protocol: | gRNA production, Phenol Chloroform precipitation | Notes: | No notes. | Results: | DNA has been used for MS2production and gRNA for cpf digest. |
Cpf1 digest -gRNA production
2018/05/29| Participants: | Keno Eilers |
| Protocol: | Cpf1 digest, Urea gel | Notes: | No notes. | Results: | No gRNA visible. |
Cpf1 digest
2018/06/06| Participants: | Keno Eilers |
| Protocol: | Cpf1 digest, Agarose gel | Notes: | No notes. | Results: | No bands visible. |
Generating new gRNA
2018/06/14| Participants: | Keno Eilers |
| Protocol: | gRNA production, Phenol Chloroform precipitation | Notes: | Used DNAse I | Results: | DNA has been used for Urea Page gel |
gRNA generation and Urea Page Gel
2018/06/18| Participants: | Keno Eilers |
| Protocol: | gRNA production, Urea gel | Notes: | No notes. | Results: |
gRNA1 620 ng/µl gRNA2 591 ng/µl |
Cpf1 digest with gRNA
2018/06/26| Participants: | Keno Eilers |
| Protocol: | Cpf1 digest, Agarose gel | Notes: | No notes. | Results: | No bands, T7 DNA not clean enough. |