Difference between revisions of "Team:USP-EEL-Brazil/Biobricks"

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We submitted four parts to the Registry (see list below ).Unfortunately, we were only able to characterize BBa_K2603003. This part is in a plasmid different from those standardized by IGEM, however, we contacted registry to be allowed to submit this part.<p>
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We successfully submitted four parts to the Registry (see list below). Unfortunately, we were only able to characterize the Lac TT (BBa_K2603003). This part is in a plasmid different from the IGEM standart, however, the Registry committee allowed us to submit and send a sample of this part.<p>
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<p style=" margin-bottom: 20px; text-align: justify;    text-indent: 5%; ">We also had problem on the 3A Assembly of one of our parts and we were not able to submit it: the Lac PH with LacI promoter. As for the composite part,
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However, we have submitted interesting and novel parts to the Registry:
 
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     • <a href="http://parts.igem.org/Part:BBa_K2603000">BBa_K2603000</a>(basic part): Laccase from <i>Pleurotus ostreatus</i> NRRL0366 with RBS (<a href="http://parts.igem.org/Part:BBa_B0030">BBa_B0030</a>). </p>
 
     • <a href="http://parts.igem.org/Part:BBa_K2603000">BBa_K2603000</a>(basic part): Laccase from <i>Pleurotus ostreatus</i> NRRL0366 with RBS (<a href="http://parts.igem.org/Part:BBa_B0030">BBa_B0030</a>). </p>

Revision as of 00:11, 18 October 2018

Our parts

We successfully submitted four parts to the Registry (see list below). Unfortunately, we were only able to characterize the Lac TT (BBa_K2603003). This part is in a plasmid different from the IGEM standart, however, the Registry committee allowed us to submit and send a sample of this part.

We also had problem on the 3A Assembly of one of our parts and we were not able to submit it: the Lac PH with LacI promoter. As for the composite part, However, we have submitted interesting and novel parts to the Registry:

    • BBa_K2603000(basic part): Laccase from Pleurotus ostreatus NRRL0366 with RBS (BBa_B0030).

    • BBa_K2603001(basic part): Laccase from Phoma sp. UHH 5-1-03 (DSM 2245) with RBS (BBa_B0030).

    • BBa_K2603002(composite part): Laccase from Pleurotus ostreatus NRRL0366 with RBS (BBa_B0030) and Regulated LacI promoter (BBa_R0010)

    • BBa_K2603003: Laccase from Thielavia terrestris with HisTag, to help in purification, and a TEV site for HisTag clevage.

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