Difference between revisions of "Team:Lambert GA/Collaborations"
Michellewu (Talk | contribs) |
Michellewu (Talk | contribs) |
||
| Line 414: | Line 414: | ||
<br><br> | <br><br> | ||
<b style="font-size: 16px;">Recyling Spin Columns</b> | <b style="font-size: 16px;">Recyling Spin Columns</b> | ||
| + | </div> | ||
<ol> | <ol> | ||
<li>Store unclean spin columns in 1M HCl solution for 4 hours or overnight (No damage if stored for longer).</li> | <li>Store unclean spin columns in 1M HCl solution for 4 hours or overnight (No damage if stored for longer).</li> | ||
Revision as of 00:17, 18 October 2018
C O L L A B O R A T I O N S
Atlanta Science Festival Collaboration with GSU | March 24, 2018
Summary Points
Atlanta Science Festival is a nonprofit organization dedicated to bringing people together through the wonders of science. This organization was founded in 2014 by Emory University, Georgia Tech, and Metro Atlanta Chamber. This event is an annual two-week festival that celebrates local science and technology, featuring more than 100 events and reaching 50,000 children and adults annually. Festival events feature hands-on activities, tours, tastings, and performances from more than 100 partner organizations. Lambert iGEM participated in the Atlanta Science Festival in order to increase the public’s knowledge of synthetic biology. Through collaboration with the Georgia State iGEM team, Lambert iGEM helped children create art with micropipettes and color dye. This simple experiment helped spark interests in science.
For more information, visit Atlanta Science Festival's website.
- Collaboration with Georgia State University
- American Sign Language Camp
- Micropipette Art
- Informing the younger generation about synthetic biology
Atlanta Science Festival is a nonprofit organization dedicated to bringing people together through the wonders of science. This organization was founded in 2014 by Emory University, Georgia Tech, and Metro Atlanta Chamber. This event is an annual two-week festival that celebrates local science and technology, featuring more than 100 events and reaching 50,000 children and adults annually. Festival events feature hands-on activities, tours, tastings, and performances from more than 100 partner organizations. Lambert iGEM participated in the Atlanta Science Festival in order to increase the public’s knowledge of synthetic biology. Through collaboration with the Georgia State iGEM team, Lambert iGEM helped children create art with micropipettes and color dye. This simple experiment helped spark interests in science.
For more information, visit Atlanta Science Festival's website.
Lambert iGEM and GSU iGEM members helping young children with micropipette painging.
Georgia State Volunteer American Sign Language (ASL) | June 12-13, 2018
Summary Points
- Collaboration with Georgia State University
- American sign language camp
- American sign language for synthetic biology teams
Lambert and GSU iGEM members run through basic biotech experiments with deaf students.
UGA Summer Camp Collaboration | July 18-20, 2018
Summary Points
The Lambert iGEM team invited the UGA iGEM team. Five UGA members came over to assist Lambert iGEM members with experiments and lectures.
UGA students, Stephan George, Chynna Pollitt, Kelton Mcconnell, & Arjun Bhatt, helped us prepare labs such as the Transformation lab and DNA lab. They spoke to us about working in a college lab and experiences that they have had.
- Students from the UGA iGEM team helped prepare lab experiments and lectures
The Lambert iGEM team invited the UGA iGEM team. Five UGA members came over to assist Lambert iGEM members with experiments and lectures.
UGA students, Stephan George, Chynna Pollitt, Kelton Mcconnell, & Arjun Bhatt, helped us prepare labs such as the Transformation lab and DNA lab. They spoke to us about working in a college lab and experiences that they have had.
Lambert and UGA iGEM members work together to introduce biotechnology and frugal science to middle school students.
Georgia iGEM Meetup | November 22, 2018
Summary Points
Lambert iGEM planned a Georgia meetup, which took place at Emory University. iGEM teams from Emory University and Georgia Southern came together. We discussed our projects and had a question and answer session. This meetup allowed us to brainstorm ideas together and to interact with other iGEM team members.
- Lambert iGEM planned a Georgia Meetup at Emory University
- iGEM members from GSU, UGA, Lambert, and Emory University
Lambert iGEM planned a Georgia meetup, which took place at Emory University. iGEM teams from Emory University and Georgia Southern came together. We discussed our projects and had a question and answer session. This meetup allowed us to brainstorm ideas together and to interact with other iGEM team members.
Lambert, GSU, and Emory iGEM members gather to discuss projects, ask questions, and give suggestions.
iGEM Team Collaboration
Taipei American School & University of Georgia
A significant component in validating the accuracy and precision of our ElectroPen was understanding the repeatability of results. The TAS and UGA teams were instrumental in proving our ElectroPen works in the real world. Both teams were not only able to successfully electroporate E. coli cells, but also obtained results similar to those from the trials conducted by Lambert's own team. We are very grateful for the collaborations between two iGEM teams without whose time and efforts we could not have proven our Electropen hardware. Thank you TASTaipei and UGA!
TASTaipei received an assembled ElectroPen from us and built a custom cuvette from protocols that we provided. Using these, they prepared electrocompetent DH5a and Nissile 1917 E. coli. They were able to successfully transform both strains with a GFP plasmid and provided transformation efficiency data. UGA’s team also received an assembled ElectroPen and cuvette from us for testing. They were also able to successfully transform and validate GFP expression with a plate reader. Both teams gave us feedback on design and ease of use of the devices.
For more on the ElectroPen, visit our ElectroPen page.
A significant component in validating the accuracy and precision of our ElectroPen was understanding the repeatability of results. The TAS and UGA teams were instrumental in proving our ElectroPen works in the real world. Both teams were not only able to successfully electroporate E. coli cells, but also obtained results similar to those from the trials conducted by Lambert's own team. We are very grateful for the collaborations between two iGEM teams without whose time and efforts we could not have proven our Electropen hardware. Thank you TASTaipei and UGA!
TASTaipei received an assembled ElectroPen from us and built a custom cuvette from protocols that we provided. Using these, they prepared electrocompetent DH5a and Nissile 1917 E. coli. They were able to successfully transform both strains with a GFP plasmid and provided transformation efficiency data. UGA’s team also received an assembled ElectroPen and cuvette from us for testing. They were also able to successfully transform and validate GFP expression with a plate reader. Both teams gave us feedback on design and ease of use of the devices.
For more on the ElectroPen, visit our ElectroPen page.
Nanjing-China
Lambert iGEM created a lab emoji in collaboration with Team Nanjing. The teams hoped to increase accessibility of science to the community. It was an activity that allowed Lambert iGEM to consider the fun of science rather than always focusing on its serious effects. This mindset set allowed a more relaxed, inviting lab environment and an overall better iGEM experience. Team Nanjing also used our app Color Q, testing and proving its functionality. They gave us great feedback on versatility and accessibility, changes that we took to heart. Here are their results:
Lambert iGEM created a lab emoji in collaboration with Team Nanjing. The teams hoped to increase accessibility of science to the community. It was an activity that allowed Lambert iGEM to consider the fun of science rather than always focusing on its serious effects. This mindset set allowed a more relaxed, inviting lab environment and an overall better iGEM experience. Team Nanjing also used our app Color Q, testing and proving its functionality. They gave us great feedback on versatility and accessibility, changes that we took to heart. Here are their results:
Team Nanjing-China shares their results.
Westminster UK
Due to limited resources, the Lambert iGEM team understands the significance of conservation and frugality in the lab. In fact, frugal science remains a foundation of our project. In collaboration with Westminster UK iGEM, the team developed a protocol to recycle spin columns without an autoclave, promoting an easy method of increased conservation in the lab.
Recyling Spin Columns
Due to limited resources, the Lambert iGEM team understands the significance of conservation and frugality in the lab. In fact, frugal science remains a foundation of our project. In collaboration with Westminster UK iGEM, the team developed a protocol to recycle spin columns without an autoclave, promoting an easy method of increased conservation in the lab.
Recyling Spin Columns
- Store unclean spin columns in 1M HCl solution for 4 hours or overnight (No damage if stored for longer).
- Fill each column with 1 ml sterile distilled H2O.
- Spin columns in microcentrifuge (inside microcentrifuge tubes or collection tubes).
- Discard the flow through.
- Repeat steps 2-4, 4 more times for a total of 5 times.
- Fill with 1 ml of QBT buffer to rebalance charges.
- Spin columns in microcentrifuge (inside microcentrifuge or collection tubes).
- Discard the flow through.
- Repeat steps 6-8, 4 more times for a total of 5 times.
Data shows that recycled spin columns yield similar results to new spin columns in minipreps.
ECUST
Lambert iGEM sent a summary of this year’s project to ECUST. The word restriction encouraged the team to formulate a concise and clear description - a crucial factor to consider when writing an abstract. Additionally, by simplifying the project description, we made it more accessible to the general public.
Lambert iGEM sent a summary of this year’s project to ECUST. The word restriction encouraged the team to formulate a concise and clear description - a crucial factor to consider when writing an abstract. Additionally, by simplifying the project description, we made it more accessible to the general public.
Uppsala
Lambert iGEM recognizes that synthetic biology requires a lot of hard work and can often result in high stress levels. This can cause extremely dedicated members to feel discouraged or “burned out”. Team Uppsala created a survey addressing this issue. The survey allowed iGEM members to recognize and to address their issues with iGEM-related stress.
Lambert iGEM recognizes that synthetic biology requires a lot of hard work and can often result in high stress levels. This can cause extremely dedicated members to feel discouraged or “burned out”. Team Uppsala created a survey addressing this issue. The survey allowed iGEM members to recognize and to address their issues with iGEM-related stress.
Exeter
Since both Lambert’s and Exeter’s 2018 iGEM project are based on water quality, the collaboration between both iGEM teams allowed members to investigate the source of the cause of poor water quality. Exeter iGEM asked for specific water quality reports in the United States. Researching this topic allowed Lambert iGEM to reevaluate this issue in the community, focusing in from the worldwide issue of clean water shortage.
Since both Lambert’s and Exeter’s 2018 iGEM project are based on water quality, the collaboration between both iGEM teams allowed members to investigate the source of the cause of poor water quality. Exeter iGEM asked for specific water quality reports in the United States. Researching this topic allowed Lambert iGEM to reevaluate this issue in the community, focusing in from the worldwide issue of clean water shortage.
Dalhousie
Since both Lambert’s and Dalhousie’s 2018 iGEM project are based on water quality, the collaboration between both iGEM teams allowed members to investigate the source of the cause of poor water quality. Dalhousie iGEM asked for specific water quality reports in the United States. Researching this topic allowed Lambert iGEM to reevaluate this issue in the community, focusing in from the worldwide issue of clean water shortage.
Since both Lambert’s and Dalhousie’s 2018 iGEM project are based on water quality, the collaboration between both iGEM teams allowed members to investigate the source of the cause of poor water quality. Dalhousie iGEM asked for specific water quality reports in the United States. Researching this topic allowed Lambert iGEM to reevaluate this issue in the community, focusing in from the worldwide issue of clean water shortage.
IIT Kanpur
IIT Kanpur’s project emphasizes the lack of clean water around the world, a major focus of Lambert iGEM’s project. This collaboration about water pollution allowed Lambert iGEM to recognize the presence and application of the project within the local community as well as worldwide.
IIT Kanpur’s project emphasizes the lack of clean water around the world, a major focus of Lambert iGEM’s project. This collaboration about water pollution allowed Lambert iGEM to recognize the presence and application of the project within the local community as well as worldwide.
HAFS
Since both Lambert’s and HAFS’s 2018 iGEM project are based on water quality, the collaboration between both iGEM teams allowed members to investigate the source of the cause of poor water quality. HAFS iGEM asked for specific water quality reports in the United States. Researching this topic allowed Lambert iGEM to reevaluate this issue in the community, focusing in from the worldwide issue of clean water shortage.
Since both Lambert’s and HAFS’s 2018 iGEM project are based on water quality, the collaboration between both iGEM teams allowed members to investigate the source of the cause of poor water quality. HAFS iGEM asked for specific water quality reports in the United States. Researching this topic allowed Lambert iGEM to reevaluate this issue in the community, focusing in from the worldwide issue of clean water shortage.
EPFL
Lambert iGEM took artistic pictures in the lab and submitted it to team EPFL’s photo competition. Art is a universal medium everyone can appreciate, and both teams believe it can expose the public to the beauty of synthetic biology.
Lambert iGEM took artistic pictures in the lab and submitted it to team EPFL’s photo competition. Art is a universal medium everyone can appreciate, and both teams believe it can expose the public to the beauty of synthetic biology.
Thessaloniki
Lambert iGEM participated in a survey with Team Thessaloniki. The survey allowed Lambert iGEM to reevaluate laboratory work, safety, and ethics in the synthetic biology field.
Lambert iGEM participated in a survey with Team Thessaloniki. The survey allowed Lambert iGEM to reevaluate laboratory work, safety, and ethics in the synthetic biology field.
Bulgaria
Lambert iGEM took “Follow Me to...” photos around the lab to show other iGEM teams Lambert’s lab environment. This collaboration allowed the iGEM community to gain a greater understanding of how other teams function.
Lambert iGEM took “Follow Me to...” photos around the lab to show other iGEM teams Lambert’s lab environment. This collaboration allowed the iGEM community to gain a greater understanding of how other teams function.
Imperial College
Lambert iGEM took the Imperial College’s survey on team communication and dynamics. Like all teams, Lambert iGEM has instances of tension and disagreement between members. Taking the survey pushed the team to be open about their issues and work through them, resulting in an improved team bond.
Lambert iGEM took the Imperial College’s survey on team communication and dynamics. Like all teams, Lambert iGEM has instances of tension and disagreement between members. Taking the survey pushed the team to be open about their issues and work through them, resulting in an improved team bond.
UC San Diego
The lab environment of synthetic biology can often be polarizing, as it is a relatively new field. Lambert iGEM participated in a survey about iGEM’s goals, needs, and climate to further solidify iGEM and synthetic biology’s direction in the future.
The lab environment of synthetic biology can often be polarizing, as it is a relatively new field. Lambert iGEM participated in a survey about iGEM’s goals, needs, and climate to further solidify iGEM and synthetic biology’s direction in the future.
US AFRL Carrol
There is a stigma associated with the biotechnology field. This negative perception shared by the public can hinder advancements in synthetic biology. To address this issue, Lambert iGEM posed with US AFRL Carrol’s characters Mike the Microbe and Chia the Chitinase, two lovable animations that appeal to the general public.
There is a stigma associated with the biotechnology field. This negative perception shared by the public can hinder advancements in synthetic biology. To address this issue, Lambert iGEM posed with US AFRL Carrol’s characters Mike the Microbe and Chia the Chitinase, two lovable animations that appeal to the general public.
Bielefeld
Lambert iGEM participated in a survey and lecture about Dual Use, a scientific concern that most iGEM team members are unfamiliar with. Through this collaboration, the team was able to recognize and understand this international ethical issue.
Lambert iGEM participated in a survey and lecture about Dual Use, a scientific concern that most iGEM team members are unfamiliar with. Through this collaboration, the team was able to recognize and understand this international ethical issue.
UCopenhagen
Lambert iGEM participated in a survey, which focused on the public engagement aspect of our team. The survey allowed members to reevaluate experiences within the team and with the public.
Lambert iGEM participated in a survey, which focused on the public engagement aspect of our team. The survey allowed members to reevaluate experiences within the team and with the public.
TecCEM
Lambert iGEM participated in a survey about burn treatment in America. The team submitted local information to contribute to TecCEM’s project.
Lambert iGEM participated in a survey about burn treatment in America. The team submitted local information to contribute to TecCEM’s project.
UNebraska-Lincoln
Lambert iGEM participated in a survey about invasive species. The team provided local specific information to help UNebraska-Lincoln’s project.
Lambert iGEM participated in a survey about invasive species. The team provided local specific information to help UNebraska-Lincoln’s project.
UNESP Brazil
Lambert iGEM completed researched on engineered probiotics, clinical studies, and federal regulations. The team provided local information to help UNESP Brazil’s project.
Lambert iGEM completed researched on engineered probiotics, clinical studies, and federal regulations. The team provided local information to help UNESP Brazil’s project.