Difference between revisions of "Team:BIT/Improve"

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<h1>Improve</h1>
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<p>For teams seeking to improve upon a previous part or project, you should document all of your work on this page. Please remember to include all part measurement and characterization data on the part page on the Registry. Please include a link to your improved part on this page.</p>
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<h3>Gold Medal Criterion #2</h3>
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<p><b>Standard Tracks:</b> Create a new part that has a functional improvement upon an existing BioBrick part. The sequences of the new and existing parts must be different. You must perform experiments with both parts to demonstrate this improvement. Document the experimental characterization on the Part's Main Page on the Registry for both the existing and new parts. Both the new and existing Main Page of each Part’s Registry entry must reference each other. Submit a sample of the new part to the Registry.
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The existing part must NOT be from your 2018 part number range and must be different from the part documented in bronze #4.
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            <h1>Improve</h1>
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            <p>We modified the sequence of the promoter BBa_I14018 , and got the promoter BBa_K2708009.
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<b>Special Tracks:</b> Improve the function of an existing iGEM project (that your current team did not originally create) and display your achievement on your wiki.</p>
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            <img src="https://static.igem.org/mediawiki/parts/5/59/T--BIT--Figure_Improve1.png">
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            <p>Then we assembled these two promoter with repoter BBa_E0840 and transforming plasmid to Ecoli DH5α, measuring green fluorescence to characterize the effect of the promoter.
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            <img src="https://static.igem.org/mediawiki/parts/d/d9/T--BIT--Figure_Improve2.png">
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                The figure below is the fluorescence results we measured in the eighth hour of culture. Excitation and emission are 480nm and 520nm, E0840 is the negative control. The results show that the promoter K2708009 has a stronger starting ability than I14018.
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            <img src="https://static.igem.org/mediawiki/parts/5/5c/T--BIT--Figure_Improve3.png">
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Revision as of 01:38, 18 October 2018

<!DOCTYPE html> Collaboration

Improve

We modified the sequence of the promoter BBa_I14018 , and got the promoter BBa_K2708009.

Then we assembled these two promoter with repoter BBa_E0840 and transforming plasmid to Ecoli DH5α, measuring green fluorescence to characterize the effect of the promoter.

The figure below is the fluorescence results we measured in the eighth hour of culture. Excitation and emission are 480nm and 520nm, E0840 is the negative control. The results show that the promoter K2708009 has a stronger starting ability than I14018.