Difference between revisions of "Team:SIAT-SCIE/Protocol"

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         <details style="margin-top: 40px;">
 
         <details style="margin-top: 40px;">
 
             <summary>PCR</summary>
 
             <summary>PCR</summary>
−
             <p>test</p>
+
            <h> PCR condition</h>
 +
             <p>
 +
<ul>
 +
<li>Before cycles: 98°C 10
 +
</li>
 +
<li>After cycles: 72°C 7min; 16°C ∞  (4°C is more suitable for storing PCR product, but 4°C overnight may damage some PCR machine)
 +
(A) For reactions in which the quantity of template is 200 ng / 50 μl or less:*
 +
</li>
 +
<li>-98°C 10 sec.
 +
</li>
 +
<li>-55°C 5 sec. or 15 sec
 +
</li>
 +
<li>-72°C 5 sec./kb
 +
</li>
 +
<li>-30-35 cycles
 +
</li>
 +
<br/>
 +
<br/>
 +
<li>(B) For reactions in which the quantity of template exceeds 200 ng / 50 μl:*
-98°C 10 sec.
 +
</li>
 +
<li>-55°C 5 sec. or 15 sec.
 +
</li>
 +
<li>-72°C 30 - 60 sec./kb
 +
</li>
 +
<li>-30-35 cycles
 +
</li>
 +
</p>
 
         </details>
 
         </details>
 
         <details>
 
         <details>

Revision as of 01:38, 18 October 2018

PCR PCR condition

  • Before cycles: 98°C 10
  • After cycles: 72°C 7min; 16°C ∞ (4°C is more suitable for storing PCR product, but 4°C overnight may damage some PCR machine) (A) For reactions in which the quantity of template is 200 ng / 50 μl or less:*
  • -98°C 10 sec.
  • -55°C 5 sec. or 15 sec
  • -72°C 5 sec./kb
  • -30-35 cycles


  • (B) For reactions in which the quantity of template exceeds 200 ng / 50 μl:*
-98°C 10 sec.
  • -55°C 5 sec. or 15 sec.
  • -72°C 30 - 60 sec./kb
  • -30-35 cycles

Agarose Gel Electrophoresis

After setup the program doesn't start