Difference between revisions of "Team:HZAU-China/Notebook"
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<p style="width: 100%; text-align: center !important;"><b>Figure 1.</b> Infection of HeLa GSDMD KO cells by <i>Salmonella</i> and the result of analysis.</p><br> | <p style="width: 100%; text-align: center !important;"><b>Figure 1.</b> Infection of HeLa GSDMD KO cells by <i>Salmonella</i> and the result of analysis.</p><br> | ||
<p>The amounts of host cells and bacteria were measured in experiments. | <p>The amounts of host cells and bacteria were measured in experiments. | ||
| − | </p | + | </p> |
</div> | </div> | ||
<div id="float02"> | <div id="float02"> | ||
| − | <p>The concentration of bacteria is | + | <p>The concentration of bacteria is calculated according to MOI (multiplicity of infection) and cell density.<br><br> |
We adjusted the parameter As to match the curve with the results of the Salmonella infection experiment. When As is | We adjusted the parameter As to match the curve with the results of the Salmonella infection experiment. When As is | ||
equal to 0.0003, the curve simulated by the mathematical model matches the experimental measurements. | equal to 0.0003, the curve simulated by the mathematical model matches the experimental measurements. | ||
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</div> | </div> | ||
<p style="width: 100%; text-align: center !important;"><b>Figure 2.</b> Parameter fitting of the tumor | <p style="width: 100%; text-align: center !important;"><b>Figure 2.</b> Parameter fitting of the tumor | ||
| − | cells infection experiments.Red points are experimental results. | + | cells infection experiments. Red points are experimental results. |
</p><br> | </p><br> | ||
<p> | <p> | ||
The parameters ‘Nsal’ and ‘Tumor’ are already known before the experiment. When the parameter ‘As’ is 0.0003, | The parameters ‘Nsal’ and ‘Tumor’ are already known before the experiment. When the parameter ‘As’ is 0.0003, | ||
| − | the model results fit the experimental results. </p> | + | the model results best fit the experimental results. </p> |
</div> | </div> | ||
Revision as of 01:38, 18 October 2018
1. Verification of Poisson distribution
We infect tumor cells (HeLa GSDMD KO cell line) with Salmonella carrying the GFP gene for a period of time and then count them, and the result is that the distribution of Salmonella in the tumor cells is consistent with a Poisson distribution.
Figure 1. Infection of HeLa GSDMD KO cells by Salmonella and the result of analysis.
The amounts of host cells and bacteria were measured in experiments.
The concentration of bacteria is calculated according to MOI (multiplicity of infection) and cell density.
We adjusted the parameter As to match the curve with the results of the Salmonella infection experiment. When As is
equal to 0.0003, the curve simulated by the mathematical model matches the experimental measurements.
Figure 2. Parameter fitting of the tumor cells infection experiments. Red points are experimental results.
The parameters ‘Nsal’ and ‘Tumor’ are already known before the experiment. When the parameter ‘As’ is 0.0003, the model results best fit the experimental results.
