Difference between revisions of "Team:TecCEM/Demonstrate"

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                     <h1>Cell Line Burn Standardization Assay </h1>
 
                     <h1>Cell Line Burn Standardization Assay </h1>
                     <p>  </p>
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                     <p> In this in vitro burn model, an increase of cell injuries and changes in cell viability and morphology is observed in relation to the time exposed to hot HBSS, compared to the controls and determined with MTT assay and microscope visualization. The damage done by the hot HBSS causes apoptosis and detachment of cells in the culture flask. This analysis allows us to proceed with the evaluation of TecTissue performing the same assay and applying the designed treatment. The importance of this standardization lies in the fact that it establishes how to perform  future assays involving burns injuries, this information will be useful for future lines of investigation regarding burn assays.</p>
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                                 <img src="https://static.igem.org/mediawiki/2018/9/93/T--TecCEM--PublicEngagement3.png" class="figure-img img-fluid rounded"
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                                 <img src="https://static.igem.org/mediawiki/2018/2/2b/T--TecCEM--Demonstrate1.png" class="figure-img img-fluid rounded"
 
                                     alt="UNIVERSUM 1">
 
                                     alt="UNIVERSUM 1">
                                 <figcaption class="figure-caption">Figure 3.</figcaption>
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                                 <figcaption class="figure-caption">Figure 1. Cell Visualization of different times of burn treatment using 60ºC HBSS  in L-929 cell line
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</figcaption>
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                                <img src="https://static.igem.org/mediawiki/2018/8/8b/T--TecCEM--PublicEngagement4.png" class="figure-img img-fluid rounded"
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                                    alt="UNIVERSUM 2">
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                                <figcaption class="figure-caption">Figure 4.</figcaption>
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Revision as of 01:39, 18 October 2018

Cell Gif

Demonstrate


Cell Line Burn Standardization Assay

In this in vitro burn model, an increase of cell injuries and changes in cell viability and morphology is observed in relation to the time exposed to hot HBSS, compared to the controls and determined with MTT assay and microscope visualization. The damage done by the hot HBSS causes apoptosis and detachment of cells in the culture flask. This analysis allows us to proceed with the evaluation of TecTissue performing the same assay and applying the designed treatment. The importance of this standardization lies in the fact that it establishes how to perform future assays involving burns injuries, this information will be useful for future lines of investigation regarding burn assays.

UNIVERSUM 1
Figure 1. Cell Visualization of different times of burn treatment using 60ºC HBSS in L-929 cell line

Burn assay with treatment

UNIVERSUM 1
Figure 3.
UNIVERSUM 2
Figure 4.

Cell Culture in BOB

UNIVERSUM 1
Figure 3.
UNIVERSUM 2
Figure 4.