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<h1>Cell Line Burn Standardization Assay </h1> | <h1>Cell Line Burn Standardization Assay </h1> | ||
− | <p> </p> | + | <p> In this in vitro burn model, an increase of cell injuries and changes in cell viability and morphology is observed in relation to the time exposed to hot HBSS, compared to the controls and determined with MTT assay and microscope visualization. The damage done by the hot HBSS causes apoptosis and detachment of cells in the culture flask. This analysis allows us to proceed with the evaluation of TecTissue performing the same assay and applying the designed treatment. The importance of this standardization lies in the fact that it establishes how to perform future assays involving burns injuries, this information will be useful for future lines of investigation regarding burn assays.</p> |
− | <div class="row my-3"> | + | <div class="row my-3 text-center"> |
− | <div class="col- | + | <div class="col-12 text-center"> |
− | <figure class="figure text- | + | <figure class="figure text-center"> |
− | <img src="https://static.igem.org/mediawiki/2018/ | + | <img src="https://static.igem.org/mediawiki/2018/2/2b/T--TecCEM--Demonstrate1.png" class="figure-img img-fluid rounded" |
alt="UNIVERSUM 1"> | alt="UNIVERSUM 1"> | ||
− | <figcaption class="figure-caption">Figure | + | <figcaption class="figure-caption">Figure 1. Cell Visualization of different times of burn treatment using 60ºC HBSS in L-929 cell line |
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</figure> | </figure> | ||
</div> | </div> |
Revision as of 01:39, 18 October 2018
Demonstrate
Cell Line Burn Standardization Assay
In this in vitro burn model, an increase of cell injuries and changes in cell viability and morphology is observed in relation to the time exposed to hot HBSS, compared to the controls and determined with MTT assay and microscope visualization. The damage done by the hot HBSS causes apoptosis and detachment of cells in the culture flask. This analysis allows us to proceed with the evaluation of TecTissue performing the same assay and applying the designed treatment. The importance of this standardization lies in the fact that it establishes how to perform future assays involving burns injuries, this information will be useful for future lines of investigation regarding burn assays.
Burn assay with treatment
Cell Culture in BOB