$("#Content15").html("<p>Materials<br><br>› LM Media with Ampicillin (with 1 uL to 1 mL of LB culture) and Glucose (1% wt/vol glucose)<br>› 50 mL falcon tube<br>› Floating plasmid inside cells in glycerol stock (MEGA plasmid in C43 in this case)<br>› IPTG (in liquid form, but varying concentrations relative to volume)<br>› 400ml conical flask<br>› 4x 150ml conical flasks<br>› Cuvettes");

         $("#Content15").html("<p>Materials<br><br>› LM Media with Ampicillin (with 1 uL to 1 mL of LB culture) and Glucose (1% wt/vol glucose)<br>› 50 mL falcon tube<br>› Floating plasmid inside cells in glycerol stock (MEGA plasmid in C43 in this case)<br>› IPTG (in liquid form, but varying concentrations relative to volume)<br>› 400ml conical flask<br>› 4x 150ml conical flasks<br>› Cuvettes");

         $("#Content16").html("<p>Procedure<br><br>1. Prepare 5 mL of media, containing a normal concentration of ampicillin (1 uL to 1 mL of LB culture), as well as 1% wt/vol glucose. Put it in a 50 mL falcon tube.<br>2. Scrape a bit of MEGA plasmid glycerol stock out of its tube, using a pipette tip. Drop this tip into the culture tube. Grow the culture up overnight in the 37C shaking incubator.<br>3. Measure the OD the next morning. Hopefully, it will have hit saturation - around an OD of 4. If it's below 4, put the tube back in the incubator to grow until saturation.<br>4. Meanwhile, make 400 mL of media, same ingredients as above (ampicillin, glucose) except at a lower glucose concentration of 0.2% (wt/vol). Put the media into a large flask. When the 5 mL culture reaches an OD of 4, pipette 4 mL of it into the flask, and shake at 37C for ~2 h.<br>5. Pull the flask right at 2 hr, or even before, and check the OD. You are aiming for an OD between 0.4 to 0.6. Keep the culture in the incubator until it reaches this range (but don't let it overgrow and go higher than 0.6 - if it gets too high, you'll have to start the whole experiment over again).<br>6. When the OD reaches 0.4 - 0.6, you'll want to induce with IPTG. First, you’ll need to divide the culture into 4 different flasks, 100 mL each. This is because, just like we did with the CFP and YFP plasmids in the plate, we want to add a different amount of IPTG to each. One of the flasks will have 0, and be our control flask, while another will have: 200 uM IPTG, 20 μM IPTG, and 2 uM IPTG (final concentrations). Just like before, you'll have to calculate to figure out how much IPTG to add to 100 mL of media to get 200 uM final concentration, and then do 1:10 serial dilutions of the stock down from there.<br>7. After adding the right amount of IPTG to each flask, change the shaking incubator temperature to 30C, and grow the flasks overnight for an additional 16–24 hours.<br>8. Look to see if the cells float! You'll also want to grow up the C43 cells as a control, so do exactly the same thing with them, except you only need to make 100 mLs of culture, not 400. Also, don't add ampicillin to them, since they aren't resistant and will die. Note: In biology, wt/vol refers to g/mL, for some reason. So, for 1% glucose solution, you need 1 gram glucose / 100 mL volume.");

         $("#Content16").html("<p>Procedure<br><br>1. Prepare 5 mL of media, containing a normal concentration of ampicillin (1 uL to 1 mL of LB culture), as well as 1% wt/vol glucose. Put it in a 50 mL falcon tube.<br>2. Scrape a bit of MEGA plasmid glycerol stock out of its tube, using a pipette tip. Drop this tip into the culture tube. Grow the culture up overnight in the 37C shaking incubator.<br>3. Measure the OD the next morning. Hopefully, it will have hit saturation - around an OD of 4. If it's below 4, put the tube back in the incubator to grow until saturation.<br>4. Meanwhile, make 400 mL of media, same ingredients as above (ampicillin, glucose) except at a lower glucose concentration of 0.2% (wt/vol). Put the media into a large flask. When the 5 mL culture reaches an OD of 4, pipette 4 mL of it into the flask, and shake at 37C for ~2 h.<br>5. Pull the flask right at 2 hr, or even before, and check the OD. You are aiming for an OD between 0.4 to 0.6. Keep the culture in the incubator until it reaches this range (but don't let it overgrow and go higher than 0.6 - if it gets too high, you'll have to start the whole experiment over again).<br>6. When the OD reaches 0.4 - 0.6, you'll want to induce with IPTG. First, you’ll need to divide the culture into 4 different flasks, 100 mL each. This is because, just like we did with the CFP and YFP plasmids in the plate, we want to add a different amount of IPTG to each. One of the flasks will have 0, and be our control flask, while another will have: 200 uM IPTG, 20 μM IPTG, and 2 uM IPTG (final concentrations). Just like before, you'll have to calculate to figure out how much IPTG to add to 100 mL of media to get 200 uM final concentration, and then do 1:10 serial dilutions of the stock down from there.<br>7. After adding the right amount of IPTG to each flask, change the shaking incubator temperature to 30C, and grow the flasks overnight for an additional 16–24 hours.<br>8. Look to see if the cells float! You'll also want to grow up the C43 cells as a control, so do exactly the same thing with them, except you only need to make 100 mLs of culture, not 400. Also, don't add ampicillin to them, since they aren't resistant and will die. Note: In biology, wt/vol refers to g/mL, for some reason. So, for 1% glucose solution, you need 1 gram glucose / 100 mL volume.");