Difference between revisions of "Team:Munich/mProteinEn.html"

 
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Assembly of T4 Phage with Yfp_his_hoc in commercial cell extract
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<em>
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2018/10/10
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<table class=”table table-borderless”>
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<td>Participants:</td>
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<td>Brigit Tunaj</td>
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<tr>
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<td>Protocol:</td>
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<td>
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<a href="https://static.igem.org/mediawiki/2018/c/c1/T--Munich--PhageT7AssemblyTXTL.pdf" target="_blank">Phage Assembly TX/TL</a>,
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<a href="https://static.igem.org/mediawiki/2018/2/23/T--Munich--AgarOverlayPlaqueAssay_.pdf" target="_blank">Agar overlay plaque assay</a>
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<td>Notes:</td>
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        <td>
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600 ng DNA and 0,54 uM yfp_his_hoc Protein
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      </td>
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<td>Results:</td>
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<td>
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Spot test  worked --> has been used fluorescence microscopy
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</table>
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Latest revision as of 01:49, 18 October 2018

Modular Protein Engineering

during Wikifreeze
Participants: Quirin Emslander
Protocol: Phage T7 DNA Purification, Phage T7 Assembly TX-TL
Notes: Overexpression of proteins
Purification and Assembly of proteins
Results: Plasmids psb1C3_T7_His_yFP_HOC and psb1C3_T7_His_Nanoluc_HOC got cloned.
Proteins His_yFP_HOC / His_Nanoluc_HOC got expressed and purified.
Phages assembled with His_yFP_HOC / His_Nanoluc_HOC

Assembly of T4 Phage with Yfp_his_hoc in commercial cell extract

2018/10/10
Participants: Brigit Tunaj
Protocol: Phage Assembly TX/TL, Agar overlay plaque assay
Notes: 600 ng DNA and 0,54 uM yfp_his_hoc Protein
Results: Spot test worked --> has been used fluorescence microscopy