Difference between revisions of "Team:IIT-Madras/Basic Part"

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<h2>Best Basic Part Special Prize</h2>
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<h1>Basic Parts</h1>
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A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
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<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
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<h3>Note</h3>
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<p>This page should list all the basic parts your team has made during your project. You must add all characterization information for your parts on the Registry. You should not put characterization information on this page. Remember judges will only look at the first part in the list for the Best Basic Part award, so put your best part first!</p>
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<h3>Best Basic Part Special Prize</h3>
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<p> To be eligible for this award, this part must adhere to <a href="http://parts.igem.org/DNA_Submission">Registry sample submission guidelines</a> and have been sent to the Registry of Standard Biological Parts. If you have a part you wish to nominate your team for this <a href="https://2018.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2018.igem.org/Judging/Judging_Form">judging form</a> and delete the box at the top of this page.
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<p> <h3>BBaK2857001</h3>
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The BBaK2857001 BioBrick contains GFP codon-optimized for use in <em>Acinetobacter baylyi</em>. The codon-optimized sequence was generated using the CUTE tool from ChassiDex. This part was cloned into the pBAV1K vector and driven by the bacteriophage T5 promoter. This pBAV1K-T5-CO-GFP construct was transformed into <em>A. baylyi</em> and fluorometry studies were carried out. It was found that the codon-optimized GFP shows considerably higher fluorescence than the pBAV1K-T5-eGFP part (BBA_K1321309), containing eGFP codon-optimized for use in <em>E. coli</em>, used as the control, thus demonstrating that CUTE functions as required and any required gene can be codon-optimized for <em>A. baylyi</em>.
 
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
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<img src="https://static.igem.org/mediawiki/parts/7/7e/GFP_correct.png"><br><br>
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The optimal excitation wavelength for the codon-optimized GFP was found to be 480 nm and the optimal emission wavelength was found to be 512 nm.<br><br>
  
  

Revision as of 01:50, 18 October 2018

iGEM Collaborations Page

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Best Basic Part Special Prize

BBaK2857001

The BBaK2857001 BioBrick contains GFP codon-optimized for use in Acinetobacter baylyi. The codon-optimized sequence was generated using the CUTE tool from ChassiDex. This part was cloned into the pBAV1K vector and driven by the bacteriophage T5 promoter. This pBAV1K-T5-CO-GFP construct was transformed into A. baylyi and fluorometry studies were carried out. It was found that the codon-optimized GFP shows considerably higher fluorescence than the pBAV1K-T5-eGFP part (BBA_K1321309), containing eGFP codon-optimized for use in E. coli, used as the control, thus demonstrating that CUTE functions as required and any required gene can be codon-optimized for A. baylyi.



The optimal excitation wavelength for the codon-optimized GFP was found to be 480 nm and the optimal emission wavelength was found to be 512 nm.