Difference between revisions of "Team:Minnesota/Results"

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{{Minnesota}}
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<h1>Results</h1>
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<p>Here you can describe the results of your project and your future plans. </p>
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<h3>What should this page contain?</h3>
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<li> Clearly and objectively describe the results of your work.</li>
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    <a id="top"></a>
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<li> Future plans for the project. </li>
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<li> Considerations for replicating the experiments. </li>
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            <span dir="auto">Team:Minnesota</span>
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<h3>Describe what your results mean </h3>
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<ul>
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<li> Interpretation of the results obtained during your project. Don't just show a plot/figure/graph/other, tell us what you think the data means. This is an important part of your project that the judges will look for. </li>
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<li> Show data, but remember all measurement and characterization data must be on part pages in the Registry. </li>
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 +
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 +
.igem_2018_menu_wrapper #display_menu_control { display:none; }
 +
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 +
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}
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/* IF THE SCREEN IS LESS THAN 800PX */
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.igem_2018_menu_wrapper { width:100%; height: 10%; position:relative; left:5%;}
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/* special class that the system uses to make sure the team wants a page to be evaluated */
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border: 4px solid #3399ff;
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<!--- THIS IS WHERE THE HTML BEGINS --->
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<head>
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<meta name="viewport" content="width=device-width, initial-scale=1">
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</head>
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<div class="igem_2018_menu_wrapper" >
 +
 +
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 +
<a href="https://2018.igem.org/Team:Minnesota">
 +
<img src="http://placehold.it/350x150">
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</a>
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<!-- this div is hidden by default and will only be displayed if the screen size is too small -->
 +
<div class="menu_button" id="display_menu_control">
 +
MENU 
 +
</div>
 +
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<div id="menu_content">
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<a href="https://2018.igem.org/Team:Minnesota">
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<div class="menu_button direct_to_page">
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HOME
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</a>
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<div class="menu_button">
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<div class="expand_collapse_icon">  </div> TEAM
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</div>
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<div class="submenu_wrapper" id="team_submenu">
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<a href="https://2018.igem.org/Team:Minnesota/Team">
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<div class="submenu_button" id="Team_page">
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Team Members
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</div>
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</a>
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<a href="https://2018.igem.org/Team:Minnesota/Collaborations">
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<div class="submenu_button"  id="Collaborations_page">
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Collaborations
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</a>
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</div>
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<div class="menu_button">
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<div class="expand_collapse_icon">  </div> PROJECT
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</div>
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<!-- project submenu -->
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<div class="submenu_wrapper">
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<a href="https://2018.igem.org/Team:Minnesota/Description">
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<div class="submenu_button"  id="Description_page">
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Description
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</div>
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</a>
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<a href="https://2018.igem.org/Team:Minnesota/Design">
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<div class="submenu_button"  id="Design_page">
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Design
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</div>
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</a>
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<a href="https://2018.igem.org/Team:Minnesota/Experiments">
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<div class="submenu_button"  id="Experiments_page">
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Experiments
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<a href="https://2018.igem.org/Team:Minnesota/Notebook">
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<div class="submenu_button"  id="Notebook_page">
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Notebook
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</div>
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</a>
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<a href="https://2018.igem.org/Team:Minnesota/InterLab">
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<div class="submenu_button"  id="InterLab_page">
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</a>
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<a href="https://2018.igem.org/Team:Minnesota/Contribution">
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<div class="submenu_button"  id="Contribution_page">
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Contribution
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</div>
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</a>
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<a href="https://2018.igem.org/Team:Minnesota/Model">
 +
<div class="submenu_button"  id="Model_page">
 +
Model
 +
</div>
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</a>
 +
 +
<a href="https://2018.igem.org/Team:Minnesota/Results">
 +
<div class="submenu_button"  id="Results_page">
 +
Results
 +
</div>
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</a>
 +
 +
 +
<a href="https://2018.igem.org/Team:Minnesota/Demonstrate">
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<div class="submenu_button"  id="Demonstrate_page">
 +
Demonstrate
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</div>
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</a>
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 +
<a href="https://2018.igem.org/Team:Minnesota/Improve">
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<div class="submenu_button"  id="Improve_page">
 +
Improve
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</div>
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</a>
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<a href="https://2018.igem.org/Team:Minnesota/Attributions">
 +
<div class="submenu_button"  id="Attributions_page">
 +
Attributions
 +
</div>
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</a>
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</div>
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<div class="menu_button">
 +
<div class="expand_collapse_icon">  </div> PARTS
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</div>
 +
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<!-- parts submenu -->
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<div class="submenu_wrapper">
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<a href="https://2018.igem.org/Team:Minnesota/Parts">
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<div class="submenu_button"  id="Parts_page">
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Parts
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</a>
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<a href="https://2018.igem.org/Team:Minnesota/Basic_Part">
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<div class="submenu_button"  id="Basic_Part_page">
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Basic Parts
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</a>
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<div class="submenu_button"  id="Composite_Part_page">
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Composite Parts
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</a>
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<div class="submenu_button"  id="Part_Collection_page">
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Part Collection
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</a>
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<a href="https://2018.igem.org/Team:Minnesota/Safety">
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<div class="menu_button direct_to_page">
 +
SAFETY
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</div>
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</a>
 +
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 +
<div class="menu_button" >
 +
<div class="expand_collapse_icon">  </div> HUMAN PRACTICES
 +
</div>
 +
 +
<!-- human practices submenu -->
 +
<div class="submenu_wrapper">
 +
 +
<a href="https://2018.igem.org/Team:Minnesota/Human_Practices">
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<div class="submenu_button"  id="Silver_page">
 +
HUMAN PRACTICES
 +
</div>
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</a>
 +
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<a href="https://2018.igem.org/Team:Minnesota/Public_Engagement">
 +
<div class="submenu_button" id="Gold_Integrated_page">
 +
Education and Engagement
 +
</div>
 +
</a>
 +
 +
</div>
 +
 +
 +
<div class="menu_button">
 +
<div class="expand_collapse_icon">  </div> AWARDS
 +
</div>
 +
 +
<!-- awards submenu -->
 +
<div class="submenu_wrapper">
 +
 +
<a href="https://2018.igem.org/Team:Minnesota/Applied_Design">
 +
<div class="submenu_button"  id="Applied_Design_page">
 +
Applied Design
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</div>
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</a>
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<a href="https://2018.igem.org/Team:Minnesota/Entrepreneurship">
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<div class="submenu_button"  id="Entrepreneurship_page">
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Entrepreneurship
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</div>
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</a>
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<a href="https://2018.igem.org/Team:Minnesota/Hardware">
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<div class="submenu_button"  id="Hardware_page">
 +
Hardware
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</div>
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</a>
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<a href="https://2018.igem.org/Team:Minnesota/Measurement">
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<div class="submenu_button"  id="Measurement_page">
 +
Measurement
 +
</div>
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</a>
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<a href="https://2018.igem.org/Team:Minnesota/Model">
 +
<div class="submenu_button"  id="Model_page">
 +
Model
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</div>
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</a>
 +
 +
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 +
<div class="submenu_button"  id="Plant_page">
 +
Plant
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</a>
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<div class="submenu_button"  id="Software_page">
 +
Software
 +
</div>
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</a>
 +
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</div>
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<a href="https://igem.org/2018_Judging_Form?team=Minnesota">
 +
<div class="menu_button direct_to_page">
 +
JUDGING FORM
 +
</div>
 +
</a>
 +
 +
 +
 +
<div class="menu_bottom_padding" >
 +
</div>
 +
 +
</div>
  
−
<div class="column two_thirds_size" >
 
−
<h3> Project Achievements </h3>
 
  
−
<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
 
  
−
<ul>
 
−
<li>A list of linked bullet points of the successful results during your project</li>
 
−
<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
 
−
</ul>
 
  
 
</div>
 
</div>
Line 50: Line 802:
  
  
−
<div class="column third_size" >
+
<!-- start of content -->
−
<div class="highlight decoration_A_full">
+
<div class="igem_2018_content_wrapper">
−
<h3>Inspiration</h3>
+
<h1> #Minnesota 2018 </h1>
−
<p>See how other teams presented their results.</p>
+
 
−
<ul>
+
 
−
<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
+
<!------------------------------------ MAKING A NAVIGATION BAR------------------------------------------>
−
<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
+
<div class="navbar">
−
<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
+
        <a href="https://2018.igem.org/Team:Minnesota">Home</a>
−
</ul>
+
 
−
</div>
+
          <div class="dropdown">
 +
            <button class="dropbtn">+Team</button>
 +
            <div class="dropdown-content">
 +
              <a href="https://2018.igem.org/Team:Minnesota/Team" target="_blank"> Team Members </a>
 +
              <a href="https://2018.igem.org/Team:Minnesota/Collaborations" target="_blank"> Collaborations </a>
 +
            </div>
 +
          </div>
 +
       
 +
        <div class="dropdown">
 +
            <button class="dropbtn">+Project</button>
 +
            <div class="dropdown-content">
 +
              <a href="https://2018.igem.org/Team:Minnesota/Description">Description</a>
 +
              <a href="https://2018.igem.org/Team:Minnesota/Design">Design</a>
 +
              <a href="https://2018.igem.org/Team:Minnesota/Experiments">Experiments</a>
 +
              <a href="https://2018.igem.org/Team:Minnesota/Notebook">Notebook</a>
 +
              <a href="https://2018.igem.org/Team:Minnesota/Model">Model</a>
 +
              <a href="https://2018.igem.org/Team:Minnesota/Results">Results</a>
 +
              <a href="https://2018.igem.org/Team:Minnesota/Attributions">Attributions</a>
 +
              <a href="https://2018.igem.org/Team:Minnesota/Interlab">Interlab</a>
 +
 
 +
            </div>
 +
        </div>
 +
       
 +
        <div class="dropdown">
 +
            <button class="dropbtn">+Parts</button>
 +
            <div class="dropdown-content">
 +
              <a href="https://2018.igem.org/Team:Minnesota/Parts">Parts</a>
 +
              <a href="https://2018.igem.org/Team:Minnesota/Basic_Part">Basic Parts</a>
 +
              <a href="https://2018.igem.org/Team:Minnesota/Composite_Part">Composite Parts</a>
 +
            </div>
 +
          </div>
 +
       
 +
        <div class="dropdown">
 +
            <button class="dropbtn">+Human Practices</button>
 +
            <div class="dropdown-content">
 +
              <a href="https://2018.igem.org/Team:Minnesota/Human_Practices">Human Practices</a>
 +
              <a href="https://2018.igem.org/Team:Minnesota/Public_Engagement">Education and Engagement</a>
 +
            </div>
 +
        </div>
 +
 
 +
 
 +
 
 +
        <a href="https://2018.igem.org/Team:Minnesota/Safety">Safety</a>
 +
        <a href="https://2018.igem.org/Team:Minnesota/Judging">Medal Requirement</a>
 +
        <a href="https://igem.org/2018_Judging_Form?team=Minnesota">JUDGING FORM</a>
 +
        <p> #MINNESOTA 2018 </p>
 
</div>
 
</div>
 +
<br/>
 +
<body>
 +
<span style="font-size:20px; color:black">RESULTS:</span>
 +
</body>
 +
<br/>
 +
 +
<style>
 +
body{text-align:center}
 +
</style>
 +
 +
 +
<span style="font-size:25px; color:black">Figure 1. Gel electrophoresis results for BamHI digested Mer-GlnA-GFP(Tn501) plasmid.
 +
</span>
 +
<br/>
 +
 +
<img src="https://static.igem.org/mediawiki/2018/8/87/T--Minnesota--gel1.jpeg" height="250" width="250" margin:20px 10px; />
 +
<br/>
 +
<br/>
 +
 +
 +
 +
<span style="font-size:25px; color:black">In figure 1, the bands on lane No. 2, 3, 6, 7, 12 represented successful plasmid construction. Comparing to the ladder, our bands have a size of roughly 7.7kb, which is the length that we are looking for.
 +
</span>
 +
<img src="https://static.igem.org/mediawiki/2018/6/61/T--Minnesota--gel2.jpeg" height="250" width="250" margin:20px 10px; />
 +
<br/>
 +
<br/>
 +
 +
<br/>
 +
 +
<span style="font-size:25px; color:black">Figure 2. Gel electrophoresis results for BamHI digested Mer-GlnA-GFP(pDu) plasmid.<br/>
 +
In figure 2, the lanes are counted in a right to left, up to down fashion.<br/> Lanes number 4, 13 and 14 show bands at roughly 7.7 kb according to our ladder separation.<br/> We also expect the length of our digested product to be at this length, so we can conclude that the plasmid construct is successful.
 +
</span>
 +
<br/>
 +
<br/>
 +
<img src="https://static.igem.org/mediawiki/2018/8/8c/T--Minnesota--rbs.png" height="250" width="250" margin:20px 10px; />
 +
 +
 +
<span style="font-size:25px; color:black">Figure 3: Gel Electrophoresis result for Mer-GlnA-GFP(pDu) plasmid
 +
</span>
 +
<br/><br/>
 +
<img src="https://static.igem.org/mediawiki/2018/0/0b/T--Minnesota--gel3.jpeg" height="250" width="250" margin:20px 10px; />
 +
<br/>
 +
<br/>
 +
<span style="font-size:25px; color:black">The length of Mer-GlnA-GFP(Tn501) plasmid should be 7.7kb. Figure 3 shows that the length of plasmids we constructed are 7.7kb. <br/>We concluded that the Mer-GlnA-GFP(pDu) plasmids were successfully constructed.
 +
</span>
 +
<br/>
 +
<br/>
 +
<img src="https://static.igem.org/mediawiki/2018/5/57/T--Minnesota--gel4.jpeg" height="250" width="250" margin:20px 10px; />
 +
<br/>
 +
<br/>
 +
<span style="font-size:25px; color:black">The length of our LacI-GlnA-GFP plasmid should be 5.5kb. Figure 4 shows that samples in well 1, 2, 6, 10, 12 have the correct length. <br/>We concluded those LacI-GlnA-GFP plasmids were successfully constructed.
 +
</span>
 +
 +
 +
<style>
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<style>
 +
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 +
        .navbar {
 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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 +
          background-color: #ddd;
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        .dropdown:hover .dropdown-content {
 +
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<style>
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          background-image: url(https://static.igem.org/mediawiki/2018/c/c5/T--Minnesota--Background2.jpeg);
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          background-size: cover;
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</p>
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Team:Minnesota

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#Minnesota 2018

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#MINNESOTA 2018


RESULTS:
Figure 1. Gel electrophoresis results for BamHI digested Mer-GlnA-GFP(Tn501) plasmid.


In figure 1, the bands on lane No. 2, 3, 6, 7, 12 represented successful plasmid construction. Comparing to the ladder, our bands have a size of roughly 7.7kb, which is the length that we are looking for.


Figure 2. Gel electrophoresis results for BamHI digested Mer-GlnA-GFP(pDu) plasmid.
In figure 2, the lanes are counted in a right to left, up to down fashion.
Lanes number 4, 13 and 14 show bands at roughly 7.7 kb according to our ladder separation.
We also expect the length of our digested product to be at this length, so we can conclude that the plasmid construct is successful.

Figure 3: Gel Electrophoresis result for Mer-GlnA-GFP(pDu) plasmid



The length of Mer-GlnA-GFP(Tn501) plasmid should be 7.7kb. Figure 3 shows that the length of plasmids we constructed are 7.7kb.
We concluded that the Mer-GlnA-GFP(pDu) plasmids were successfully constructed.



The length of our LacI-GlnA-GFP plasmid should be 5.5kb. Figure 4 shows that samples in well 1, 2, 6, 10, 12 have the correct length.
We concluded those LacI-GlnA-GFP plasmids were successfully constructed.

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