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One of the major objectives of our project is to make a Synthetic promoter library for Acinetobacter baylyi ADP1. This can very helpful to scientists working on synthetic biology and metabolic engineering areas. Traditional approaches work on extremes of knocking out a gene or over expression. However, in Synthetic biology experiments, we require stringent promoters that have tight control over the gene expression rate. | One of the major objectives of our project is to make a Synthetic promoter library for Acinetobacter baylyi ADP1. This can very helpful to scientists working on synthetic biology and metabolic engineering areas. Traditional approaches work on extremes of knocking out a gene or over expression. However, in Synthetic biology experiments, we require stringent promoters that have tight control over the gene expression rate. | ||
<p> | <p> | ||
− | We have taken T5-GFP-pBAV1k(BBa_K1321309) as control and normalized the fluorometry data with respect of it. | + | We have taken T5-GFP-pBAV1k(BBa_K1321309) as control and normalized the fluorometry data with respect of it.<br><br> |
− | The parts belonging to this collection are: | + | The parts belonging to this collection are:<br> |
− | < | + | P71S: BBa_K2857110<br> |
− | + | P6S: BBa_K2857106<br> | |
− | + | P5R: BBa_K2857115<br> | |
− | + | P5S: BBa_K2857105<br> | |
− | + | P2S: BBa_K2857102<br> | |
− | + | P71R: BBa_K2857120<br> | |
− | + | P4R: BBa_K2857114<br> | |
− | + | P6R: BBa_K2857116<br> | |
− | + | P70R: BBa_K2857117<br> | |
− | + | P8R: BBa_K2857118<br> | |
− | + | P9R: BBa_K2857119<br> | |
− | + | P1R: BBa_K2857111<br> | |
− | + | P1S: BBa_K2857101<br> | |
− | + | P3R: BBa_K2857113<br> | |
− | The results are summarized in the bar graph below. | + | The results are summarized in the bar graph below.<br> |
<img src="https://static.igem.org/mediawiki/parts/9/96/T--IIT-Madras-Promoter-characterization.png" style="width:inherit;"> | <img src="https://static.igem.org/mediawiki/parts/9/96/T--IIT-Madras-Promoter-characterization.png" style="width:inherit;"> | ||
</html> | </html> |
Latest revision as of 02:57, 18 October 2018
Part Collection
One of the major objectives of our project is to make a Synthetic promoter library for Acinetobacter baylyi ADP1. This can very helpful to scientists working on synthetic biology and metabolic engineering areas. Traditional approaches work on extremes of knocking out a gene or over expression. However, in Synthetic biology experiments, we require stringent promoters that have tight control over the gene expression rate.
We have taken T5-GFP-pBAV1k(BBa_K1321309) as control and normalized the fluorometry data with respect of it.
The parts belonging to this collection are:
P71S: BBa_K2857110
P6S: BBa_K2857106
P5R: BBa_K2857115
P5S: BBa_K2857105
P2S: BBa_K2857102
P71R: BBa_K2857120
P4R: BBa_K2857114
P6R: BBa_K2857116
P70R: BBa_K2857117
P8R: BBa_K2857118
P9R: BBa_K2857119
P1R: BBa_K2857111
P1S: BBa_K2857101
P3R: BBa_K2857113
The results are summarized in the bar graph below.