Difference between revisions of "Team:Kyoto/Model"

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    <span class="box-title"><font face="Segoe UI">Table of contents</font></span>
 
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            <li><a href="#Boil Method">1) Boil Method</a></li>
 
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            <li><a href="#SLiCE">2) SLiCE</font></a></li>
 
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<h5 id="Boil Method"><font face="Segoe UI">1)Boil Method</font></h5>
 
  
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<h5 id="Abstract"><font face="Segoe UI">1)Abstract</font></h5>
  
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<p>We have to optimize combination of recombinant genes and amount of yeast when we apply our salt up taking system to real environment. But these are nontrivial because of cytosolic sodium ions’ inhibition of growth or alteration of intracellular Na⁺’s flux. We tried to make mathematical model that gives initial value corresponding to objective amount of salt decrease. We described yeast cell’s kinetics of salt absorbance. Then, using this model, we looked for best combination of recombinant genes. Through experiments, we calculated yeast’s behavior when the yeast has single protein in this project. We decided kinetic parameter of transporter with our model. Finally, we simulated possible pattern of recombinant yeast in our project. </p>
  
  

Revision as of 03:03, 18 October 2018

Team:Kyoto/Project - 2018.igem.org

1)Abstract

We have to optimize combination of recombinant genes and amount of yeast when we apply our salt up taking system to real environment. But these are nontrivial because of cytosolic sodium ions’ inhibition of growth or alteration of intracellular Na⁺’s flux. We tried to make mathematical model that gives initial value corresponding to objective amount of salt decrease. We described yeast cell’s kinetics of salt absorbance. Then, using this model, we looked for best combination of recombinant genes. Through experiments, we calculated yeast’s behavior when the yeast has single protein in this project. We decided kinetic parameter of transporter with our model. Finally, we simulated possible pattern of recombinant yeast in our project.